Antibodies to phosphatidylinositol 4 5 bisphosphate pip2

Antibodies to RyR (pan-specific), AHCY, AHCYL1, and β-actin, and mouse IgG-κ binding protein conjugated to CFL 488 were from Santa Cruz Biotechnology (Dallas, TX). Goat anti-mouse IgG conjugated to IRDye 680RD and goat anti-rabbit IRDye 800CW were from LI-COR (Lincoln, NE). Antibodies to phosphatidylinositol 4,5 bisphosphate (PIP₂) were from Echelon Biosciences (Salt Lake City, UT). Goat anti-mouse IgG conjugated to horseradish peroxidase was from BioRad (Hercules, CA). ERK1/2 and pERK1/2 antibodies were from Cell Signaling Technology (Danvers, MA). Oligonucleotides encoding gRNA and primers were obtained from Integrated DNA Technologies (Coralville, IA). The pSpCas9(BB)-2A-Puro (PX459) V2.0 vector was a gift from Feng Zhang (Addgene plasmid # 62988). T4 polynucleotide kinase and T7 DNA ligase were from New England Biolabs (Ipswich, MA). Fast AP thermosensitive alkaline phosphatase and FastDigest Bpil were from Thermo Scientific (Waltham, MA). Plasmid-Safe ATP-Dependent DNase was from Lucigen (Middleton, WI). Surveyor Mutation Detection Kit S100 was from Integrated DNA Technologies (Coralville, IA).
Chemicals- Fura-2 AM was from Invitrogen (Carlsbad, CA). Xestospongin C was from Cayman Chemical (Ann Arbor, MI). All other reagents, unless otherwise indicated, were from Sigma-Aldrich (St. Louis, MO).

Fura-2 AM was from Molecular Devices (San Jose, CA). Xestospongin C and rapamycin were from Cayman Chemical (Ann Arbor, MI). Bethanechol was from Alfa Aesar (Haverhill, MA). Apamin was from Tocris Bioscience (Bristol, UK). Stim1 antibody and mouse IgG-κ Fc binding protein conjugated to CFL 488 were from Santa Cruz Biotechnology (Dallas, TX). Secondary antibody (goat anti-mouse IgG conjugated to horseradish peroxidase) was from BioRad (Hercules, CA). Antibodies to phosphatidylinositol 4,5 bisphosphate (PIP₂) were from Echelon Biosciences (Salt Lake City, UT). All other reagents, unless otherwise indicated, were from Sigma-Aldrich (St. Louis, MO). Phalloidin conjugates were from Biotium (Fremont, CA). Plasmids encoding Pseudojanin (PJ) (Addgene plasmid # 37999), LYN11-FRB-CFP (Addgene plasmid # 38003), and PJ-DEAD (PJ-D) (Addgene plasmid # 38002) were gifts from Robin Irvine. GFP-C1-PLCdelta-PH was a gift of Tobias Meyer (Addgene plasmid # 21179).