Log In Sign up
The largest database of trusted experimental protocols

Anti cd4 clone l 200

Manufactured by BD
Visit Supplier

The Anti-CD4 (clone L-200) is a monoclonal antibody that binds to the CD4 receptor on the surface of T cells. It is a laboratory research tool used to identify and study CD4-positive cells in various biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd8 clone sk1, by BD (2 mentions) Anti cd3 clone ucht1, by BD (2 mentions) Anti cd27 clone l128, by BD (2 mentions) Anti cd38 clone ls 198, by Beckman Coulter (2 mentions) Anti cd45ra clone abb11, by Beckman Coulter (2 mentions) Anti hla dr clone l243, by BioLegend (2 mentions) Facs lysing solution, by BD (2 mentions) Lsrii flow cytometer, by BD (2 mentions)

Close spelling variants of this product

Anti-CD4

2 protocols using anti cd4 clone l 200

1

Whole Blood Flow Cytometry Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
A whole blood flow cytometry technique was employed as previously described [47 (link)]. Whole-blood samples (200ul) were stained at room temperature for 20 minutes. The following directly conjugated monoclonal antibodies (mAbs) were used: anti-CD3 (clone UCHT1, BD Biosciences), anti-CD4 (clone L-200, BD Biosciences), anti-CD8 (clone SK1, BD Biosciences), anti-CD27 (clone L128, BD Biosciences), anti-CD38 (clone LS-198, Beckman Coulter), anti-CD45RA (clone AbB11, Beckman Coulter) and anti-HLA-DR (clone L243, Biolegend). Red cells were lysed and fixed in FACS lysing solution (BD Biosciences) for 10 minutes in the dark, washed twice in FACS buffer (phosphate-buffered saline (PBS) containing 2% bovine serum albumin and 0.1% NaN3), and fixed with 1% formalin in PBS. Cells were acquired on an LSR-II flow cytometer (BD Biosciences).
Kalokhe A.S., Ibegbu C.C., Kaur S.P., Amara R.R., Kelley M.E., del Rio C, & Stephenson R. (2016). Intimate Partner Violence is Associated with Increased CD4+ T-Cell Activation Among HIV-Negative High-Risk Women. Pathogens & Immunity, 1(1), 193-213.
+ Open protocol
+ Expand
2

Whole Blood Flow Cytometry Method

Check if the same lab product or an alternative is used in the 5 most similar protocols
A whole blood flow cytometry technique was employed as previously
described [47 (link)]. Whole-blood samples
(200ul) were stained at room temperature for 20 minutes. The following directly
conjugated monoclonal antibodies (mAbs) were used: anti-CD3 (clone UCHT1, BD
Biosciences), anti-CD4 (clone L-200, BD Biosciences), anti-CD8 (clone SK1, BD
Biosciences), anti-CD27 (clone L128, BD Biosciences), anti-CD38 (clone LS-198,
Beckman Coulter), anti-CD45RA (clone AbB11, Beckman Coulter) and anti-HLA-DR
(clone L243, Biolegend). Red cells were lysed and fixed in FACS lysing solution
(BD Biosciences) for 10 minutes in the dark, washed twice in FACS buffer
(phosphate-buffered saline (PBS) containing 2% bovine serum albumin and
0.1% NaN3), and fixed with 1% formalin in PBS. Cells
were acquired on an LSR-II flow cytometer (BD Biosciences).
Kalokhe A.S., Ibegbu C.C., Kaur S.P., Amara R.R., Kelley M.E., del Rio C, & Stephenson R. (2016). Intimate Partner Violence is Associated with Increased CD4+ T-Cell Activation Among HIV-Negative High-Risk Women. Pathogens & Immunity, 1(1), 193-213.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!