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6 protocols using ammonium hydroxide

1

Alginate-Chitosan Hydrogel Synthesis

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Alginate (sodium salt from brown algae, 4–12 cP, bioreagent), chitosan (50–150 kDa molecular weight, >80% degree of deacetylation, from algae), cerium chloride, calcium chloride, N-hydroxy-succinimide (NHS), Gelatin (type B, from bovine skin), and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) were obtained from Sigma-Aldrich. Ammonium hydroxide and hydrogen chloride were obtained from BDH.
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2

Quantification of Opioid Compounds

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Morphine, Morphine-d3, 6-MAM, 6-monoacetylMorphine-d3 (6-MAM-d3), codeine, codeine-d3, and 6-AC were purchased from Lipomed (Arlesheim, Switzerland). Methanol (HPLC grade), acetonitrile (HPLC grade), ammonium carbonate, formic acid, and ammonium hydroxide were obtained from BDH (Poole, UK). Ammonium formate was obtained from Sigma-Aldrich (Steinheim, Germany). Clean Screen® solid phase extraction (SPE) cartridges (CSDAU203) were obtained from United Chemical Technologies (Bristol, PA, USA).
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3

Synthesis of Metal-Doped Polymer Nanocomposites

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Polyvinylpyrrolidone (Mw 10 kDa), cadmium nitrate (Cd(NO3)2·4H2O, 99%), lead nitrate (Pb(NO3)2, 99%), nickel nitrate (Ni(NO3)2·6H2O, 99%), potassium dichromate (K2CrO7, 99%) were purchased from Sigma-Aldrich. FeCl3·6H2O (>98%) and ammonium hydroxide (NH4OH, 25–30%) were purchased from BDH and FeCl2·4H2O (98%) from Alfa Aesar. All chemicals were used as received without further purification.
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4

Synthesis of Iron Oxide Nanoparticles

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The metallic precursors iron(II) chloride (FeCl2), iron(III) chloride (FeCl3), N,N’-diisopropylcarbodiimide (DIC), 4-dimethylaminopyridine (DMAP), dimethyl sulfoxide (DMSO), dichloromethane (DCM), dimethylformamide (DMF), Pd black, sodium ascorbate, sodium carbonate (Na2CO3) and dextrans were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ammonium hydroxide (28–30 wt %) was purchased from BDH (Poole, Dorset, UK). Poly(ethylene glycol) 6000 (PEG 6000) was purchased from Alfa Aesar (Heysham, UK). Sodium phosphate monobasic and dibasic were purchased from Amresco (Solon, OH, USA). Tribenzyloxybenzoic acid (TBBA) was purchased from Tokyo Chemical Industry. N2 purged deionized (DI) water was used throughout the entire experiment. All chemicals used were of analytical grade.
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5

Synthesis of Hydroxyapatite and Copper Nanoparticles

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Calcium nitrate tetrahydrate
(Ca(NO3)2·4H2O, Lachema Brno),
ammonium dihydrogen orthophosphate (NH4·H2PO4, ADWIC), ammonium hydroxide (NH4OH, 25%
NH3, BDH AnalaR), and cetyltrimethylammonium bromide (CTAB,
Merck) were used without additional purification in the 1D hydroxyapatite
(1D HAP) synthesis. Copper nitrate trihydrate (Cu(NO3)2·3H2O, assay: 98%, Laboratory Chemicals) and
ascorbic acid (assay: 98%, Alpha Chemicals) were used for the synthesis
of Cu NPs. 4-NP (assay: 98%) and sodium borohydride (NaBH4, assay: 98%) were obtained from Research-Lab Fine Chem Industries
(India) and SDFCL, respectively. Sigma-Aldrich (Germany) provided
the organic dyes (Congo red and acriflavine hydrochloride, ACF).
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6

Synthesis of 3-Formylchromone

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3-Formylchromone (Scheme 1) was synthesized using a method described in the literature.25 Ni(OAc)2·4H2O, Ni(NO3)2·6H2O, Ni(ClO4)2·6H2O, NiCl2·6H2O, Co(OAc)2·4H2O, Co(NO3)2·6H2O, CoCl2·6H2O, 8-hydroxyquinoline, oxine (8-HQ), 1,10-phenanthroline, Na2EDTA, nitric acid, mureoxide, and ammonium hydroxide were purchased from either BDH or Merck. The organic solvents were directly employed without distillation and were reagent grade compounds.
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