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Lightsheet z 1 selective plane illumination microscope

Manufactured by Zeiss
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The Lightsheet Z.1 is a Selective Plane Illumination Microscope (SPIM) manufactured by Zeiss. It uses a thin sheet of light to illuminate the sample, allowing for optical sectioning and reduced phototoxicity. The Lightsheet Z.1 enables high-speed, high-resolution imaging of living specimens.

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Lab products found in correlation

A9045, by Merck Group (1 mentions) Tricaine, by Merck Group (1 mentions) Fc500, by Beckman Coulter (1 mentions)

Spelling variants of this product

Lightsheet Z.1 (105 citations) Lightsheet Z.1 microscope (79 citations) Lightsheet microscope (5 citations) Lightsheet Z.1 lightsheet microscope (2 citations)

2 protocols using lightsheet z 1 selective plane illumination microscope

1

Fluorescence Imaging of Zebrafish Embryos

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Zebrafish embryos at 3–5 dpf were anesthetized using 0.16 mg/ml tricaine and then mounted in 1.5% low-melting agarose (Sigma–Aldrich, A9045) using a glass capillary for fluorescence imaging by using a Zeiss Lightsheet Z.1 Selective Plane Illumination Microscope with or without a time-lapse mode as previously described27 .
Chen X.K., Kwan J.S., Wong G.T., Yi Z.N., Ma A.C, & Chang R.C. (2022). Leukocyte invasion of the brain after peripheral trauma in zebrafish (Danio rerio). Experimental & Molecular Medicine, 54(7), 973-987.
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2

Fluorescence Imaging and Flow Cytometry of Zebrafish Embryos

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Zebra sh embryos at 4-6 dpf were anesthetized using 0.16 mg/ml tricaine (Sigma-Aldrich) and then mounted in 1.5% low-melting agarose using glass capillary for uorescence imaging by Zeiss Lightsheet Z.1 Selective Plane Illumination Microscope with or without a time-lapse mode. For ow cytometry, the head region of WT or Tg(mepg1:GFP) zebra sh embryos was isolated and then homogenized and ltered to get the cells suspension. Beckman Coulter FC 500 was utilized to quantify the number and percentage of GFP + cells under various conditions.
Chen X., Kwan J., Wong G.T.C., H K.M.M., Yi Z., Chun‐Hang A., & Chang R.C. (2020). IL-1β-mediated macrophage infiltration into the brain after peripheral tissue injury.
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