To assess the effect of IFN-γ on the cell-surface phenotype of chordoma cells, cells were untreated or treated with 50 ng/mL of IFN-γ (R&D Systems, Minneapolis, MN) for 24 h. Cells were then harvested and stained with the following antibodies: HLA-ABC-FITC (BD Biosciences, San Jose, CA), PD-L1-APC (clone 29E.2A3; BioLegend, San Diego, CA), CD133-PE (Miltenyi Biotec, San Diego, CA), CD24-PerCP-Cy 5.5 (BD Biosciences), CD15-V450 (BD Biosciences), and ALDHA1-FITC (USBiological, Salem, MA). Cell viability was examined using far red fluorescent reactive dye (Thermo Fisher, Waltham, MA). Cells were incubated with the antibodies for 30 min at 4°C, acquired on a FACSCalibur flow cytometer or FACSVerse (Becton Dickinson, Franklin Lakes, NJ), and analyzed using FlowJo software (TreeStar, Inc., Ashland, OR). Isotype control staining was < 5% for all samples analyzed.
Cd15 v450
Manufactured by BD
The CD15-V450 is a laboratory equipment product. It is designed to detect and analyze CD15 antigen expression on cells using flow cytometry. The product utilizes a fluorescent V450 dye to label the CD15 antigen.
Automatically generated - may contain errors
Lab products found in correlation
Facscalibur flow cytometer, by BD (1 mentions)
Cd133 pe, by Miltenyi Biotec (1 mentions)
Facsverse, by BD (1 mentions)
Hla abc fitc, by BD (1 mentions)
Ifn γ, by R&D Systems (1 mentions)
Cd24 percp cy5, by BD (1 mentions)
Cd45 v500, by BD (1 mentions)
Cd56 pe cy7, by BD (1 mentions)
Cd13 pe, by BD (1 mentions)
Diva software, by BD (1 mentions)
Cd16 apc h7, by BD (1 mentions)
Cd64 apc h7, by BD (1 mentions)
Kaluza software, by Beckman Coulter (1 mentions)
Cd117 apc, by BD (1 mentions)
Cd33 pe cy7, by BD (1 mentions)
Cd38 v450, by BD (1 mentions)
Cd117 yr145, by Cell Marque (1 mentions)
Cd36 apc, by BD (1 mentions)
Cd34 percp cy5, by BD (1 mentions)
Cd71 mrq 48, by Cell Marque (1 mentions)
2 protocols using cd15 v450
1
Chordoma Cell Phenotype Changes by IFN-γ
2
Immunohistochemistry and Flow Cytometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunohistochemistry. Immunohistochemical analysis was performed on 4 μm, formalin-fixed, paraffin-embedded sections in all cases. A broad immunohistochemical panel was utilized in the evaluation of the cases. Primary antibodies included CD34 (QBEnd/10, Leica (Novocastra)), CD117 (YR145, Cell Marque), CD71 (MRQ-48, Cell Marque), E-Cadherin (4A2C7, Life Technologies), Myeloperoxidase (Dako), Hemoglobin (Cell Marque), Glycophorin A (GA-R2, Ventana), CD61 and TP53 (DO-7, Ventana).
Flow cytometry. After isotonic erythrocyte lysis, flow cytometric immunophenotyping was performed on anticoagulated bone marrow aspirate specimens using previously described methods [10 (link)]. Samples were examined with flow cytometric immunophenotyping using two eight-color tubes containing antibodies from BD Biosciences (Tube1: CD13 PE, CD15 V450, CD16 APC-H7, CD33 PE-Cy7, CD34 PerCP-Cy5.5, CD45 V500, CD117 APC, HLA-DR FITC and Tube2: CD2 FITC, CD7 PE, CD34 PerCP-Cy5.5, CD36 APC, CD38 V450, CD45 V500, CD56 PE-Cy7, CD64 APC-H7). A total of 100, 000 events were collected per case. The data were analyzed using Kaluza software (Beckman-Coulter, Brea, CA) and/or Diva software (BD Biosciences).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!