Nipmam

Manufactured by Merck Group

Sourced in United States, Germany

NIPMAM is a laboratory equipment product manufactured by Merck Group. It is designed for specialized applications and functions in research and scientific laboratories. The core function of NIPMAM is to facilitate specific laboratory processes, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Sodium dodecyl sulfate (sds), by Merck Group (5 mentions) N n methylenebisacrylamide, by Merck Group (4 mentions) Nipam, by Merck Group (4 mentions) Arium 611 di water purification system, by Sartorius (2 mentions) Ammonium persulfate, by Merck Group (2 mentions) N isopropylmethacrylamide, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Milli q, by Merck Group (2 mentions) Milli ro, by Merck Group (2 mentions) Avanti j 301 centrifuge, by Beckman Coulter (1 mentions) N n methylenbisacrylamide, by Merck Group (1 mentions) Sodium dodecyl sulfate (sds), by Carl Roth (1 mentions) Methacryloxyethyl thiocarbamoyl rhodamine b, by Polysciences (1 mentions) Nile blue acrylamide, by Polysciences (1 mentions) Sodium citrate tribasic dihydrate, by Merck Group (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Ammonium persulfate, by Loba Chemie (1 mentions) Hela cells, by National Centre for Cell Science (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Thermo Fisher Scientific (1 mentions) Calcein am, by Thermo Fisher Scientific (1 mentions)

11 protocols using nipmam

Synthesis of NIPMAM Polymer Particles

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For the core particle synthesis the cross-linker N,N′-methylenbisacrylamide (BIS, 3.85 mmol, Sigma-Aldrich, USA), sodium dodecyl sulfate (SDS, 0.49 mmol, Carl Roth, Germany) and 500 mL purified water are mixed in a 1 L three-neck flask, heated up to 70 °C and stirred for 90 minutes while purged with N₂. After 85 minutes the monomer N-isopropylmethacrylamide (NIPMAM, 38.5 mmol, Sigma Aldrich, USA) is added. The polymerization is initiated by ammonium persulfate (APS, 0.31 g, ≥98%, Sigma Aldrich, USA). The solution is stirred at 70 °C for additional 4 hours at 300 rpm. Afterwards it is cooled down to room temperature and stirred over night.
The particles are purified by five times centrifugation (Beckman-Coulter Avanti™ J-301 Centrifuge, rotor: JA-30.50, USA, 15 000 rpm (27216 G), 20 °C, 30 minutes), decantation and redispersion with purified water.

Otto P., Bergmann S., Sandmeyer A., Dirksen M., Wrede O., Hellweg T, & Huser T. (2019). Resolving the internal morphology of core–shell microgels with super-resolution fluorescence microscopy. Nanoscale Advances, 2(1), 323-331.

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Synthesis of Deuterated NIPMAM Microgels

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The detailed syntheses of N-isopropylmethacrylamide (NIPMAM, Sigma-Aldrich, purity > 97%) microgels has been described elsewhere [15 (link)]. Here we also used D7-N-isopropylmethacrylamide (D7-NIPMAM, Polymer Source. Inc., Montreal, Quebec, Canada) and D12-N-isopropylmethacrylamide (D12-NIPMAM, Polymer Source. Inc., Montreal, Quebec, Canada) as the monomers. The syntheses of (partially) deuterated microgels were similar to the one with NIPMAM. For all syntheses, we performed a precipitation polymerization under nitrogen atmosphere in 50 mL water. The concentration of the monomer was always 0.082 mol·L⁻¹. The cross-linker was N,N-methylenebisacrylamide (BIS, 99%, 0.0082 mol·L⁻¹), the initiator was ammonium peroxodisulfate (>98%, 0.0027 mol·L⁻¹), and the surfactant was sodium dodecyl sulfate (>99%, 0.0049 mol·L⁻¹). All non-deuterated chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). The different chemical structures of the (partially) deuterated monomers are shown in Scheme 1.
Furthermore, we synthesized co-polymer microgels of NIPMAM and D7-NIPMAM in different ratios. For the co-monomer synthesis, the two monomers were added at the same time. The deuterated/hydrogenated ratios were 0/100, 25/75, 50/50, 75/25, and 100/0.

Cors M., Wiehemeier L., Oberdisse J, & Hellweg T. (2019). Deuteration-Induced Volume Phase Transition Temperature Shift of PNIPMAM Microgels. Polymers, 11(4), 620.

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Synthesis of Fluorescent Polymer Hydrogels

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N-Isopropylmethacrylamide
(97%, NIPMAM), the cross-linker N,N′-methylenebis(acrylamide) (99%, BIS), the surfactant sodium
dodecyl sulfate (SDS), the initiator ammonium persulfate (98% APS)
and polyethylenimine (PEI, branched, Mw 25.000 g/mol) were purchased
from Sigma-Aldrich, Zwijndrecht, The Netherlands. The dyes methacryloxyethyl
thiocarbamoyl rhodamine B (MRB) and Nile blue acrylamide (NBA) were
purchased from Polysciences, Inc., Hirschberg, Germany. N-Isopropylmethacrylamide was recrystallized from hexane; all other
chemicals were used as received without any further purification.
Ultrapure water (18.2 MΩ, arium 611 DI water purification system;
Sartorius AG, Göttingen, Germany) was used in all experiments.

Keskin D., Mergel O., van der Mei H.C., Busscher H.J, & van Rijn P. (2018). Inhibiting Bacterial Adhesion by Mechanically Modulated Microgel Coatings. Biomacromolecules, 20(1), 243-253.

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Stimuli-Responsive Nanogel for Targeted Doxorubicin Delivery

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Gold(III) chloride trihydrate (HAuCl₄), sodium citrate tribasic dihydrate, amine-terminated pNIPAm
(average M_n 2500), NIPAm, and NIPMAm were
obtained from Sigma-Aldrich. Sodium dodecyl sulfate (SDS) was obtained
from Merck. Ammonium persulfate (APS) and BIS were obtained from Loba
Chemie. Doxorubicin hydrochloride (Adriamycin) was procured from Pfizer.
HeLa cells were obtained from the National Centre for Cell Sciences,
Pune, India. Dulbecco’s modified Eagle medium (DMEM, high glucose),
Dulbecco’s phosphate-buffered saline, calcein AM, trypsin,
FBS, and MTT were procured from Thermo Fisher Scientific.

Deshpande S., Sharma S., Koul V, & Singh N. (2017). Core–Shell Nanoparticles as an Efficient, Sustained, and Triggered Drug-Delivery System. ACS Omega, 2(10), 6455-6463.

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Synthesis and Characterization of Thermoresponsive Polymers

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N-isopropylacrylamide (NIPAM; Sigma-Aldrich Chemie GmbH Munich, Germany; purity 97%) and N-isopropylmethacrylamide (NIPMAM; Sigma-Aldrich, Chemie GmbH, Munich, Germany; purity 97%) were purified by recrystallisation from hexane. The cross-linker N,N’-methylenebisacrylamide (BIS; Sigma-Aldrich Chemie GmbH, Munich, Germany; purity 99%), the initiator ammonium persulfate (APS; Sigma-Aldrich Chemie GmbH, Munich, Germany; purity ≥ 98%) and pyrene (Sigma-Aldrich Chemie GmbH, Munich, Germany; purity ≥ 99%) were used without further purification. For all experiments, purified water from an Arium®pro VF system (Sartorius AG, Göttingen, Germany) was used.
N-n-propylacrylamide (NNPAM) was synthesized via a Schotten–Baumann reaction published by Hirano et al. [21 ]. For this reaction, acryloylchloride (Sigma-Aldrich Chemie GmbH, Munich, Germany; purity 98%), n-propylamine (Fluka, Sigma-Aldrich Chemie GmbH, Munich, Germany; purity 99%), triethylamine (Grüssing GmbH Analytika, Filsum, Germany; purity 99%) and methylenchloride (p.a.) were used as received. The obtained monomer NNPAM was washed with NaHCO

_{3}

(10 wt %) and dried over MgSO

_{4}

. After filtration, the solvent was evaporated and the product was distilled in vacuum (115

^{°}

C, 10 mbar).

Wedel B., Hertle Y., Wrede O., Bookhold J, & Hellweg T. (2016). Smart Homopolymer Microgels: Influence of the Monomer Structure on the Particle Properties. Polymers, 8(4), 162.

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Preparation of Fluorescent Polymer Nanoparticles

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The monomers used were styrene (99%, contains
4-tert-butylcatechol as polymerization inhibitor,
Sigma-Aldrich), N-isopropylacrylamide (97%,
NIPAm, Sigma-Aldrich), and N-isopropylmethacrylamide
(97%, NIPMAm, Sigma-Aldrich). The inhibitor was removed with active
basic Al₂O₃ (for chromatography, VWR Chemicals).
Surfactants used were sodium dodecyl sulfate (99%, SDS, Duchefa) and
cetyltrimethylammonium bromide (99%, CTAB, Sigma-Aldrich). Dyes
used were pyrromethene 546 (PM546, Exciton) and pyrromethene 605 (PM605,
Exciton). Initiators used were potassium persulfate (99%, KPS, Sigma-Aldrich)
and 2,2-azobis(2-methylpropionamidine) dihydrochloride (97%,
V50, Sigma-Aldrich). Cross-linker was N,N′-methylene-bis-acrylamide (99%, BIS, Sigma-Aldrich).

Immink J.N., Bergman M.J., Maris J.J., Stenhammar J, & Schurtenberger P. (2020). Crystal-to-Crystal Transitions in Binary Mixtures of Soft Colloids. ACS Nano, 14(11), 14861-14868.

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Synthesis of NIPAM-NIPMAM Hydrogels

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N-isopropylacrylamide (NIPAM, TCI Germany GmbH, Eschborn, Germany, 97%) and N-isopropylmethacrylamide (NIPMAM, Sigma Aldrich, St. Louis, MO, USA, 97%) were recrystallized from n-hexane (VWR International, Darmstadt, Germany, p.a.). N,N′-methylenebisacrylamide (BIS, 99%), sodium dodecyl sulfate (SDS, 99%) and ammonium peroxodisulfate (APS, 99%) were purchased from Sigma-Aldrich (Munich, Germany) and used without further purification. Cannabidiol (CBD) was obtained as a sample from Cannovum AG (Berlin, Germany) and used as obtained.

Dirksen M., Kinder T.A., Brändel T, & Hellweg T. (2021). Temperature Controlled Loading and Release of the Anti-Inflammatory Drug Cannabidiol by Smart Microgels. Molecules, 26(11), 3181.

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Synthesis of Polymer Precursors NIPAM and NIPMAM

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NIPAM (97%; Sigma-Aldrich, Munich, Germany)
and NIPMAM (97%; Sigma-Aldrich Munich, Germany) were recrystallized
from n-hexane. Acryloylchloride (98%; Sigma-Aldrich
Munich, Germany), n-propylamine (99%, Fluka; Buchs,
Switzerland), triethylamine (99%; Grüssing, Filsum, Germany),
dichloromethane (p.A.), ammonium persulfate (≥98%; Sigma-Aldrich
Munich, Germany), N,N′-methylenebisacrylamide
(99%; Sigma-Aldrich Munich, Germany), SDS (≥99%; Sigma-Aldrich
Munich, Germany), and SDeS (≥99%; Sigma-Aldrich Munich, Germany)
were used without purification. Water was purified using an Arium
pro VF system (Satorius Stedim Systems GmbH, Göttingen, Germany).
The synthesis of NnPAM was described elsewhere.^{29 (link),30 (link),37 (link)}

Wedel B., Brändel T., Bookhold J, & Hellweg T. (2017). Role of Anionic Surfactants in the Synthesis of Smart Microgels Based on Different Acrylamides. ACS Omega, 2(1), 84-90.

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Synthesis and Characterization of NIPMAM Nanogels

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The monomer N-isopropylmethacrylamide (97%, NIPMAM), the cross-linker N,N′-methylene bis(acrylamide) (99%, BIS), the surfactant sodium dodecyl sulfate (SDS), the initiator ammonium persulfate (98% APS) and polyethyleneimine (PEI, branched, Mw 25,000 g/mol) were purchased from Sigma-Aldrich, the Netherlands. N-isopropylmethacrylamide was recrystallized from hexane; all other chemicals were used as received without purification. Ultrapure water (18.2 MΩ, arium 611 DI water purification system; Sartorius AG, Germany) was used in nanogels synthesis and coatings preparation.

Sójka O., Keskin D., van der Mei H.C., van Rijn P, & Gagliano M.C. (2023). Nanogel-based coating as an alternative strategy for biofilm control in drinking water distribution systems. Biofouling, 39(2).

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Synthesis of Polymeric Nanoparticles

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Materials N-Isopropylmethacrylamide (NIPMAM; 97%, Aldrich), N,N′methylenebisacrylamide (BIS; 99%, Fluka) monomers, sodium dodecyl sulfate (SDS; 99%, Fluka), potassium peroxodisulfate (KPS; 99%, Fluka), and polyvinyl alcohol (PVA; 89-98 kDa; 99%; Aldrich) were used as received. Styrene monomers (BASF) were purified on an Al 2 O 3 column prior to use. Water was purified using reverse osmosis (MilliRO; Millipore) and ion exchange (MilliQ; Millipore).

Crassous J.J., Mihut A.M., Månsson L.K, & Schurtenberger P. (2015). Anisotropic responsive microgels with tuneable shape and interactions. Nanoscale, 7(38).

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