Prior to all experiments, hamsters were anesthetized via intraperitoneal injections of 160 mg/kg ketamine and 7.5 mg/kg xylazine (Patterson Veterinary, Greeley, CO, USA, 07-890-8598 and 07-808-1947, respectively). Animals were kept warm on heating pads and remained surgically sedated throughout the experiment. Subcutaneous injections of aqueous atipamezole, 0.75 mg/kg (Patterson Veterinary, 07-867-7097), were used to wake up the animals.
Atipamezole
Manufactured by Performance Health
Atipamezole is a veterinary pharmaceutical product used as a reversal agent for the sedative effects of the alpha-2 adrenergic agonist medetomidine. It is primarily utilized in laboratory and research settings to quickly and safely reverse the sedative effects of medetomidine in animals.
Automatically generated - may contain errors
2 protocols using atipamezole
1
Anesthesia and Sedation in Hamsters
2
Electroretinography in Dark-Adapted Mice
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Scotopic and photopic electroretinograms were performed on mice that were dark-adapted overnight. Each mouse was anesthetized using intraperitoneal (IP) injections of 100 mg/kg of ketamine and 15 mg/kg xylazine (ketamine; KetaVed from Patterson Veterinary, Greeley, CO; xylazine from Patterson Veterinary, Greeley, CO). Once anesthetized, the pupils were dilated with proparacaine (1%; Akorn Inc., Ann Arbor, MI) and tropicamide (1% Tropicamide Opthalmic Solution, USP; Akorn Inc., Ann Arbor, MI or 0.5% Tropicamide Opthalmic Solution, USP, Sandoz, Princeton, NJ) eye drops, which were administered topically. Mice were placed on a heating pad under red light and function was analyzed with Diagnosys Celeris System (Diagnosys, LLC, Lowell, MA). Full field ERGs were assessed at the following stimulus intensities (0.001, 0.005, 0.01, 1, and 10 cd s/m2). Scotopic a-, b-, and c-waves were collected. Afterwards, mice were injected with reversal agent (0.5 mg/mL atipamezole, injection volume 5 μL per gram mouse weight; Patterson Veterinary, Greeley, CO) and placed individually in cages on top of heated water pads to recover.
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