TAK-242 (CAS Number: 243984-11-4; molecular formula, C15H17ClFN04S; molecular weight, 361.82) was supplied by MedChemExpress Company (Princeton, NJ, USA). Polydatin (CAS Number: 65914-17-2; purity, 95%, molecular formula, C20H22O8; molecular weight 390.38) and Oil Red O (cat.no. 00625) obtained from Sigma-Aldrich (St. Louis, MO, USA). P. sibiricum polysaccharides (cat.no.A16GS145378, purity 70%) was obtained from Shanghai Yuanye Biotechnology Co., Ltd. The mouse ICAM-1 (cat.no. JYM0003Mo) and VCAM-1 (cat.no. JYM0251Mo) ELISA kits were purchased from Wuhan ColorfulGene Biological Technology Co., Ltd. Hematoxylin-eosin Dye kit (cat.no. KGAA224), Ecl chemiluminescence kit (cat.no. KPG1121), Rabbit Anti-GAPDH (cat.no. KGAA002), and HRP-linked anti-rabbit IgG (cat.no. KGAA35) were obtained from Jiangsu KeyGEN Biotechnology Co., Ltd. Rabbit Anti-TLR4 (cat.no. 19811-1-AP) was obtained from Proteintech (Rosemont, IL, USA). Rabbit Anti-MyD88 (cat.no. ab219413), Rabbit Anti-p65 (cat.no. ab32536), and Rabbit Anti-p-p65 (cat.no. ab76302) were supplied by Abcam (Cambridge, UK). Nitrocellulose (NC) membrane (cat.no.6648) was obtained from Pall (New York, USA).
Rabbit anti tlr4
Manufactured by Proteintech
Sourced in United States
Rabbit Anti-TLR4 is a primary antibody that recognizes the Toll-like receptor 4 (TLR4) protein. TLR4 is a pattern recognition receptor that plays a crucial role in the innate immune response by detecting lipopolysaccharide (LPS), a component of the cell wall of Gram-negative bacteria. This antibody can be used in various applications, such as Western blotting, immunohistochemistry, and flow cytometry, to detect and analyze the expression and distribution of TLR4 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Polydatin, by Merck Group (1 mentions)
Rabbit anti p p65, by Abcam (1 mentions)
Oil red o, by Merck Group (1 mentions)
Tak 242, by MedChemExpress (1 mentions)
Imagequant las 4000 camera, by GE Healthcare (1 mentions)
Mouse anti actin, by Merck Group (1 mentions)
Puerarin, by Meilun (1 mentions)
Propidium iodide, by Abcam (1 mentions)
Hematoxylin eosin h e staining kits, by Nanjing Jiancheng (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Merck Group (1 mentions)
Annexin 5 fitc, by Abcam (1 mentions)
Hydrogen peroxide, by Meilun (1 mentions)
4 protocols using rabbit anti tlr4
1
TAK-242 and Polydatin Anti-Inflammatory Effects
2
Western Blotting of HMGB1 and TLR4
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Western blot analysis was conducted from PFC extracts as previously described.32 Membranes were probed with primary antibodies (rabbit anti‐HMGB1 1/1000, abcam #ab18256; rabbit anti‐TLR4 1/500, Proteintech [Rosemont, IL, USA] #19811; mouse anti‐actin 1/5000, Sigma‐Aldrich [Saint Louis, MO, USA] #A3854) overnight at 4C, then probed with HRP‐conjugated secondary antibody for 1 h at room temperature. Membranes were developed using enhanced chemiluminescence (ECL), and images were obtained with ImageQuant LAS 4000 camera system (GE Healthcare, Chicago, IL, USA). Band intensities were quantified using ImageJ software (NIH).
3
Neuroprotective effects of puerarin
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Puerarin (purity > 98%), cobalt chloride (CoCl2), hydrogen peroxide and 2′,7′-dichlorofluorescin diacetate (DCFH-DA) were purchased from Dalian Meilun Biotech Co., Ltd. (Dalian, China). Salvianolic acid B (purity > 95%) was obtained from Shanghai Institute of Materia Medica (Shanghai, China), 5,5-Dimethyl-1-pyrroline N-oxide, 2,3,5-triphenyltetrazolium chloride (TTC), interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor (TNF-α) ELISA kits were provided by Neobio Company (Shanghai, China). Hematoxylin-eosin (H&E) staining kits were purchased from the Nanjing Jiancheng Institute of Biotechnology (Nanjing, China). Primer was obtained from Huajin Company (Shanghai, China). Rabbit anti-TLR4, rabbit anti-MyD88 rabbit anti-SIRT1 and rabbit anti-NF-κB were obtained from Proteintech, Inc. (Wuhan, China). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were purchased from Sigma Aldrich (USA). Propidium iodide (PI) and annexin V-FITC were purchased from BioVision Inc. (Milpitas, CA, USA). PC12 cells were purchased from the Cell Bank at Chinese Academy of Sciences (Shanghai, China), Adult male Sprague-Dawley rats (250–300 g) were purchased from Sino-British BK Lab. Animal Co., Ltd. (Shanghai, China). The animal experiment protocol was approved by the Animal Experimentation Ethics Committee of Fudan University.
4
Examining Hippocampal Protein Markers in HIBD
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In this stage, 6 neonatal rats in each group were randomly selected to undergo a heart perfusion with phosphate-buffered saline under anesthesia at 24, 48, and 72 hours after HIBD, and the left hippocampus was quickly removed. We conducted tissue homogenation, centrifugation, and protein extraction. Then, we took 50 μg of protein from each sample and separated the protein via 10% or 15% sodium dodecyl sulfate polyacrylamide gel electrophoresis before transferring the samples onto a polyvinylidene fluoride membrane. We used 5% skim milk powder to perform blocking for 2 hours, added the following primary antibodies: rabbit anti-TLR4 (1:500, Proteintech), rabbit anti-phospho-NF-κB p65 (1:1000, ABclonal), rabbit anti-Beclin-1 (1:1000, MBL), and rabbit anti-LC3 (1:750, MBL), and incubated the samples overnight at 4°C. The next day, the samples were incubated with secondary goat anti-rabbit antibody (1:5000, ABclonal) for 2 hours at room temperature, and then developed and fixed via enhanced chemiluminescence in a dark room. Protein expression levels were assessed in terms of the ratio of gray values between the target protein bands and the reference protein bands using Image J software.
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