Mouse ifn γ elisa kit

Manufactured by Elabscience

The Mouse IFN-γ ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse interferon-gamma (IFN-γ) levels in biological samples such as cell culture supernatants, serum, and plasma.

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Lab products found in correlation

Mouseil 10 elisa kit, by Elabscience (1 mentions) Mouse il 12 elisa kit, by Elabscience (1 mentions) Protease inhibitor cocktail, by Apexbio (1 mentions) Mouse il 12 elisa kit, by R&D Systems (1 mentions)

Close spelling variants of this product

IFN-γ ELISA kit IFN-γ Mouse ELISA kit ELISAs

5 protocols using mouse ifn γ elisa kit

Measuring Mouse IFN-γ Levels

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IFN-γ levels were measured using a mouse IFN-γ ELISA kit (Elabscience), as described by the manufacturer.

Li Z., Zhang Y., Hong W., Wang B., Chen Y., Yang P., Zhou J., Fan J., Zeng Z, & Du S. (2022). Gut microbiota modulate radiotherapy-associated antitumor immune responses against hepatocellular carcinoma Via STING signaling. Gut Microbes, 14(1), 2119055.

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Mouse Serum IFN-γ Quantification

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The level of IFN-γ in mouse serum was analyzed using the Mouse IFN-γ ELISA Kit (Elabscience, Wuhan, China) according to the manufacturer’s instructions.

Lei M., Liu J., Gao Y., Dai W., Huang H., Jiang Q, & Liu Z. (2024). DPP Inhibition Enhances the Efficacy of PD-1 Blockade by Remodeling the Tumor Microenvironment in Lewis Lung Carcinoma Model. Biomolecules, 14(4), 391.

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Serum Inflammatory Factors ELISA Assay

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Serum matrix metalloproteinase-9 (MMP-9) and inflammatory factors, including interleukins-12 (IL-12), IL-10, interferon-γ (IFN-γ), and IL-4, were measured using the following ELISA kits (Elabscience): Mouse MMP-9 ELISA kit (Cat No. E-EL-M3052), Mouse IL-12 ELISA kit (Cat No. E-EL-M0726c), Mouse IL-10 ELISA kit (Cat No. E-EL-M0046c), Mouse IFN-γ ELISA kit (Cat No. E-EL-M0048c), and Mouse IL-4 ELISA kit (Cat No. E-EL-M0043c). All procedures followed the manufacturer's instructions.

Li Z., Ma D., Wang Y., Wu S., Wang L., Jiang Y., Zhang Y, & Li X. (2022). Astragali Radix-Coptis Rhizoma Herb Pair Attenuates Atherosclerosis in ApoE-/- Mice by Regulating the M1/M2 and Th1/Th2 Immune Balance and Activating the STAT6 Signaling Pathway. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 7421265.

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Cytokine Quantification in Tumor Tissues

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For in vivo assays, tumor tissues were minced, homogenized, and subjected to beads heating in PBS with protease inhibitor cocktail (K1007, APExBIO) on day 3 or day 6 after completion of treatment. For in vitro tests, culture media were collected by centrifugation. Quantification of cytokines in tumors and media was performed by Mouse IL-12 ELISA Kit (M1270, R&D) and Mouse IFN-γ ELISA Kit (E-EL-M0048c, Elabscience) following the manufacturer’s instruction, respectively.

Wang R., Chen J., Wang W., Zhao Z., Wang H., Liu S., Li F., Wan Y., Yin J., Wang R., Li Y., Zhang C., Zhang H, & Cao Y. (2022). CD40L-armed oncolytic herpes simplex virus suppresses pancreatic ductal adenocarcinoma by facilitating the tumor microenvironment favorable to cytotoxic T cell response in the syngeneic mouse model. Journal for Immunotherapy of Cancer, 10(1), e003809.

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Inhibiting CD8+ T cell glycolysis

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The method for CD8⁺ T cell sorting and co-culturing was the same as described previously. For the inhibition of CD8⁺ T cell glycolysis, AZ-PFKFB3-67 (MedChemExpress, USA) was used at the concentration of 5μM, 10 μM and 20μM respectively for 24h after cell sorting and for another 24 hours after glucose supplementation. The culture supernatant was collected, and the content of IFN-γ was measured using Mouse IFN-γ ELISA Kit (Elabscience, Wuhan).

Wu Y., Han W., Tang X., Liu J., Guo Z., Li Z., Cai C, & Que L. (2024). B7-H3 suppresses CD8+ T cell immunologic function through reprogramming glycolytic metabolism. Journal of Cancer, 15(9), 2505-2517.

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