Cd146 p1h12

Manufactured by BioLegend

Sourced in United States

CD146(P1H12) is a mouse monoclonal antibody that recognizes the CD146 antigen. CD146 is a cell adhesion molecule expressed on the surface of endothelial cells, smooth muscle cells, and some types of stem cells.

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CD146 (26 citations) CD146; clone P1H12 (2 citations)

2 protocols using cd146 p1h12

Characterization of Mesenchymal Stem Cells

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Single cell suspensions of MSCs were stained for the following antibodies: CD34(MOPC-173) (BioLegend, San Diego, CA, USA), CD146(P1H12) (BioLegend), CD73(AD2) (Thermo Fisher Scientific), CD90(5E10) (Thermo Fisher Scientific) and CD105(SN6) (Thermo Fisher Scientific). Following surface marker staining, cells were fixed for 10 min in 2% paraformaldehyde and were analyzed using a FACS CANTO II (BD Biosciences, Franklin Lakes, NJ, USA). For lentiviral transduction efficiency assessment, cells were not stained, but directly fixed and analyzed using the same equipment. Data was analyzed using FlowJo software (BD Biosciences).

Teryek M., Jadhav P., Bento R, & Parekkadan B. (2023). 3D Microcapsules for Human Bone Marrow Derived Mesenchymal Stem Cell Biomanufacturing in a Vertical-Wheel Bioreactor. bioRxiv.

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Comprehensive PBMC Phenotypic Analysis

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Phenotypic analysis was performed on PBMCs using panels containing Live/Dead Blue (Life technologies), CD1c (AD5-8E7; Miltenyi), CD3 (SP34-2 and SK7; BD), CD10 (HI10a; Biolegend), CD11c (B-Ly6, BD), CD14 (M5E2, BD), CD16 (3GE, Biolegend), CD19 (HIB19; Biolegend), CD20 (L27, BD), CD34 (561, Biolegend), CD45RA (HI100, Biolegend), CD56 (HCD56, BD), CD62L (SK11, BD), CD66abce (TET2, Miltenyi Biotec), CD80 (L307.4, BD), CD86 (2331; BD); CD123 (7G3; BD), CD133 (7, Biolegend), CD141 (AD5-14H12; Miltenyi), CD146 (P1H12; Biolegend), HLA-DR (TU36, Life technologies), CCR2 (K036C2, Biolegend), CCR4 (L291H4, Biolegend), CCR6 (11A9, BD) and CCR7 (150503, BD) (Panels summarized in S3 Table). Fixation was performed with 1% paraformaldehyde. Samples were acquired on an LSRFortessa flow cytometer (BD Biosciences) and analyzed using FlowJo software (Tree Star).

Vangeti S., Strandin T., Liu S., Tauriainen J., Räisänen-Sokolowski A., Cabrera L., Hassinen A., Mäkelä S., Mustonen J., Vaheri A., Vapalahti O., Klingström J, & Smed-Sörensen A. (2021). Monocyte subset redistribution from blood to kidneys in patients with Puumala virus caused hemorrhagic fever with renal syndrome. PLoS Pathogens, 17(3), e1009400.

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