All fly stocks except for RNAi experiments were cultured at 25 ± 1°C on a standard corn-yeast-sucrose medium under constant humidity and a 12:12 h light to dark cycle. The sec3GT and sec3PBac lines were described previously (Wan et al., 2013 (link)). The line hs-flp; ubi-GFP FRT80B was obtained from the Bloomington Drosophila Stock Center (BDSC). Females of the latter line were crossed to males of sec3GT and sec3PBac lines, respectively. Progeny were heat shocked for 2 h per day at 37°C for 2 days before eclosion and 1 day after eclosion, then dissected 2–3 days after the last heat shock. Progeny with genotypes of hs-flp; sec3GT FRT80B/ubi-GFP FRT80B or hs-flp; sec3PBac FRT80B/ubi-GFP FRT80B were analyzed. The sec3 mutant clones were marked by the absence of GFP. The sec3 RNAi line was obtained from the Vienna Drosophila RNAi Center (#108085) and c306-Gal4 from BDSC. The c306-Gal4>sec3-RNAi progeny were shifted to 29°C for 3–4 days before dissection.
C306 gal4
Manufactured by BD
The C306-Gal4 is a laboratory equipment product that serves a specific function. It is a genetic driver that can be used to express genes of interest in specific cell types or tissues in model organisms. The core function of the C306-Gal4 is to facilitate targeted gene expression, allowing researchers to study the role of particular genes in biological processes.
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Lab products found in correlation
C355 gal4, by BD (1 mentions)
Mgst1kg04713, by BD (1 mentions)
Su p ey13245, by BD (1 mentions)
P23ey05607, by BD (1 mentions)
Akr1bpl00034, by BD (1 mentions)
Akr1bey07011, by BD (1 mentions)
P23 rnai hmj24151, by BD (1 mentions)
P23 rnai 2 gl01292, by BD (1 mentions)
Uas dicer2, by BD (1 mentions)
2 protocols using c306 gal4
1
Genetic Manipulation of sec3 in Drosophila
2
Drosophila Genetics: Protocols and Stock Utilization
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Fly stocks were maintained on cornmeal/agar/yeast food at 21°C, except where noted. Before immunofluorescence staining, newly eclosed flies were fed wet yeast paste every day for 2–4 days. Unless specified, yw (BDSC 1495) was used as the control. The following stocks were obtained from the Bloomington Drosophila Stock Center: c355 GAL4 (BDSC 3750), c306 GAL4 (BDSC 3743), mgst1KG04713 (BDSC 13839), Su(P)EY13245 (BDSC 20866), p23EY05607(BDSC 16661), akr1BPL00034 (BDSC 19594), akr1BEY07011 (BDSC 16777), p23 RNAi HMJ24151 (BDSC 62911), p23 RNAi-2 GL01292 (BDSC 41862), akr1B RNAi HMS05657 (BDSC 67838), akr1B RNAi-2 HMC05226 (BDSC 62219) and UAS Dicer 2 (BDSC 24651). The following stocks were obtained from the Exelexis Stock Center: mgst1d10243, akr1Bd00405 and pxtf01000 (Thibault et al., 2004 (link)). The oskar GAL4 line (second chromosome; BDSC 44241) was a generous gift from Anne Ephrussi (European Molecular Biology Laboratory; (Telley et al., 2012 (link))). Expression of the RNAi lines were achieved by crossing to c355 GAL4 or oskar GAL4, maintaining fly crosses at 21°C and maintaining progeny at 29°C for 5–6 days. UAS Dicer was used in combination with c355 to enhance RNAi efficiency were noted in the figure legends.
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