Log In Sign up
The largest database of trusted experimental protocols

Explus growth media

Manufactured by Corning
Visit Supplier

ExPlus growth media is a specialized culture medium designed for the cultivation of a wide range of cell types, including mammalian, insect, and microbial cells. It provides a balanced and optimized blend of nutrients, growth factors, and other essential components to support the proliferation and maintenance of diverse cell lines.

Automatically generated - may contain errors

Lab products found in correlation

Explus growth media, by STEMCELL (2 mentions) Rat tail collagen type 1 coated permeable transwell membrane supports, by Corning (2 mentions) Pneumacult ali maintenance media, by STEMCELL (2 mentions)

2 protocols using explus growth media

1

Differentiation of Airway Epithelial Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
hTert-immortalized human normal airway tracheobronchial epithelial cells, BCi.NS1.1 (58 (link)), were maintained in ExPlus growth media (StemCell). To generate HAEC, BCi.NS1.1 cells were plated (7.5E4 cells/well) on rat-tail collagen type 1-coated permeable transwell membrane supports (6.5 mm; Corning Inc.), immersed in ExPlus growth media in both the apical and the basal chambers. Upon reaching confluence, the medium in the apical chamber was removed (airlift), and the medium in the basal chamber was changed to Pneumacult ALI maintenance media (StemCell). Pneumacult ALI maintenance medium was changed every 2 days for approximately 6 weeks to form differentiated, polarized cultures that resemble in vivo pseudostratified mucociliary epithelium.
Lokugamage K.G., Hage A., de Vries M., Valero-Jimenez A.M., Schindewolf C., Dittmann M., Rajsbaum R, & Menachery V.D. (2020). Type I Interferon Susceptibility Distinguishes SARS-CoV-2 from SARS-CoV. Journal of Virology, 94(23), e01410-20.
+ Open protocol
+ Expand
2

Differentiation of Immortalized Human Airway Epithelial Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
hTert-immortalized human normal airway tracheobronchial epithelial cells, BCi.NS1.1 (55 (link)) were maintained in ExPlus growth media (StemCell). To generate HAEC, BCi.NS1.1 were plated (7.5E4 cells/well) on rat-tail collagen type 1-coated permeable transwell membrane supports (6.5mm; Corning Inc), immersed in ExPlus growth media in both the apical and basal chamber. Upon reaching confluency, media in the apical chamber was removed (airlift), and media in the basal chamber was changed to Pneumacult ALI maintenance media (StemCell). Pneumacult ALI maintenance media was changed every two days for approximately 6 weeks to form differentiated, polarized cultures that resemble in vivo pseudostratified mucociliary epithelium.
Lokugamage K.G., Hage A., de Vries M., Valero-Jimenez A.M., Schindewolf C., Dittmann M., Rajsbaum R, & Menachery V.D. (2020). Type I interferon susceptibility distinguishes SARS-CoV-2 from SARS-CoV.. bioRxiv.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!