Goat anti rabbit igg antibody

Manufactured by Vector Laboratories

Goat anti-rabbit IgG antibody is a secondary antibody produced in goats and purified. It is designed to detect and bind to rabbit immunoglobulin G (IgG) antibodies, allowing for their identification and quantification in various immunoassays and research applications.

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Lab products found in correlation

Vectastain abc hrp kit, by Vector Laboratories (1 mentions) Image lab software, by Bio-Rad (1 mentions) Ecl prime western blotting detection reagent, by Cytiva (1 mentions) Immobilon p, by Merck Group (1 mentions) Rabbit anti bdnf antibody, by Santa Cruz Biotechnology (1 mentions) Chemidoc mp imaging system, by Bio-Rad (1 mentions) Vectastain elite abc system, by Vector Laboratories (1 mentions) Superfrost plus slide, by Thermo Fisher Scientific (1 mentions) Sk 4100, by Vector Laboratories (1 mentions) Normal goat serum (ngs), by Biosynth (1 mentions)

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Goat anti-rabbit antibody IgGs (2 citations)

3 protocols using goat anti rabbit igg antibody

Quantification of Intraepidermal Nerve Fiber Density

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Mouse hind paws were fixed in Zamboni’s fixative for 24–48 hours at 4°C and processed as previously described (62 (link)). 50 μm floating tissue sections were stained with the primary antibody rabbit anti-protein-gene-product 9.5 (PGP 9.5) (Dako, Z5116, 1:500) overnight and the secondary biotinylated goat anti-rabbit IgG antibody (Vector Laboratories, BA-1000–1.5) for 1 hour, followed by colorimetric detection using the VECTASTAIN ABC-HRP Kit (Vector Laboratories, PK-4000). Quantification of IENFD was performed following the protocol described by Lauria et al (62 (link)).

O’Brien J., Niehaus P., Remark J., Salimian M., Kevas Y., Rubin S., Kristian T., Chandrasekaran K., Pei-Ju Lu C., Russell J.W, & Ho C.Y. (2023). Epidermal SIRT1 and BDNF modulate mechanical allodynia in mouse models of diabetic neuropathy. bioRxiv.

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ProBDNF Western Blot Analysis

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ProBDNF digestion samples were run on 15% SDS-PAGE gels and electro-transferred onto polyvinylidene difluoride membrane (Immobilon-P, Millipore), using semi-dry method. Membranes were blocked for 2 hours at room tempertaure with 10% (w/v) dried non-fat milk powder in Tris-buffered saline with 0.1% Tween 20 (TBS-T). After blocking, the membranes were incubated at 4°C overnight with rabbit anti-BDNF antibody (Santa Cruz) diluted 1:500 in 5% (w/v) non-fat milk in TBS-T. Membranes were then incubated for 2 hours at room temperature with horseradish-peroxidase-labelled secondary antibody (Goat Anti-Rabbit IgG Antibody; Vector Laboratories) diluted 1:10,000 in 5% dried non-fat milk powder in TBS-T. After washing, the peroxidase activity was visualized on photographic film with ECL Prime Western Blotting Detection Reagent (Cytiva). The developed film was later digitized on ChemiDoc MP Imaging System (Bio-Rad) and analyzed using Image Lab software (Bio-Rad).

Legutko D., Kuźniewska B., Kalita K., Yasuda R., Kaczmarek L, & Michaluk P. (2024). BDNF signaling requires Matrix Metalloproteinase-9 during structural synaptic plasticity. bioRxiv.

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Immunohistochemical Staining of Fos-expressing Neurons

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40 μm brain sections were collected using a Microm HM 440E
freezing microtome and were stored in 1× PBS with 0.05%
sodium azide until immunohistochemical staining. Sections underwent 3 washes
in 1× PBS, 10-minute incubation in 1% sodium hydroxide in
1× PBS, and 3 washes in 1× PBS with 0.5% Triton-X
(Sigma) (PBST) before treatment with 5% normal goat serum
(Fitzgerald; Acton, MA) in PBST for 1 hour at room temperature. Sections
were then incubated for 48 hours in primary rabbit polyclonal anti-Fos
antibody (1:1,000; Calbiochem PC38) on an orbital shaker at 4°C.
Following primary incubation sections were washed 5 times in 1× PBS,
once in 1× PBST, and were incubated in secondary biotinylated goat
anti-rabbit IgG antibody (1:500; Vector Labs BA-1000) for 2 hours. After
secondary incubation, sections were treated with an avidin-biotin peroxidase
system (Vectastain Elite ABC System; Vector Labs: PK-6100) for 1 hour before
staining with a Nickel-DAB peroxidase substrate kit (Vector Labs; SK-4100).
All sections were dehydrated in a series of increasingly concentrated
ethanol solutions (5 minutes in 70% EtOH, 10 minutes in fresh
95% EtOH twice, 10 minutes in fresh 100% EtOH twice), bathed
in Xylenes (15 minutes in Xylenes twice), mounted onto Superfrost Plus
slides (Fisher Scientific; 12-550-150) while still partially wet, and
coverslipped using Krystalon (EMD Chemicals Inc., Gibbstown, NJ).

Johnson Z.V., Walum H., Jamal Y.A., Xiao Y., Keebaugh A.C., Inoue K, & Young L.J. (2015). Central oxytocin receptors mediate mating-induced partner preferences and enhance correlated activation across forebrain nuclei in male prairie voles. Hormones and behavior, 79, 8-17.

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