M780lp1

Manufactured by Thorlabs

Sourced in United States

The M780LP1 is a laser diode module that emits light at a wavelength of 780 nanometers. It is designed to provide a stable and collimated output beam.

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Lab products found in correlation

T2 upright microscope, by Olympus (1 mentions) Fb750 40, by Thorlabs (1 mentions) Hl6738mg, by Thorlabs (1 mentions) Nanozoomer, by Hamamatsu Photonics (1 mentions) Matlab, by MathWorks (1 mentions) Pcie 6321, by National Instruments (1 mentions)

7 protocols using m780lp1

Chronos-Expressing Cell Photostimulation

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Healthy CHO cells were identified using transmitted oblique-contrast infrared light (M780LP1 or M850L3, Thorlabs). Following this, target cells were exposed to a 5 ms blue LED light pulse to check for GFP and consequently Chronos expression. If the cell was both healthy and Chronos-expressing, it was patch clamped as described in section 2.2.
During voltage-clamp recordings we interleaved sets of red (644 nm average wavelength) and blue (480 nm average wavelength) photostimulation trials, beginning with a set of blue pulses to check for the presence of photocurrents. LED intensities stepped between 0.28 and 11.3 mW mm⁻² were randomised and deployed onto the patched cells, each with a pulse duration of 5 ms. If photocurrents fell to below 90% of the values measured in the first set, this was seen as a marker of declining cell health and/or patch quality, and subsequent trials were excluded from the final analysis. LED control signals and photocurrents were recorded in Spike2, and analysed with custom-written python scripts.

Soor N.S., Quicke P., Howe C.L., Pang K.T., Neil M.A., Schultz S.R, & Foust A.J. (2019). All-optical crosstalk-free manipulation and readout of Chronos-expressing neurons. Journal of Physics D, 52(10), 104002.

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Large Volume Scattering Imaging System

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The large volume scattering imaging system (Fig. 1A and Supporting Information Fig. S1) consists of an 800 mW, 780 nm infrared (IR, Bandwidth: 30 nm) LEDs (M780LP1, Thorlabs, Inc., USA), with collimating and focusing lens and a central blocking aperture to focus a ring-shaped illumination through the sample or the reference cuvettes. Wide-view and deep field depth scattering images were recorded by a CMOS camera (BFS-U3–16S2M-CS, Point Grey Research Inc., Canada) at 65 fps through the variable zoom lenses (NAVITAR 12X, Navitar, USA) with zoom factors set at 2.0X for the sample cuvettes. The image volume was determined by the viewing size and focal depth of the optics. For the experiments described in this study, the viewing volume of 2.5 mm × 1.9 mm × 1.0 mm was equivalent to 4.8 μL at 2.0X magnifying power. The sample cuvette was placed on a temperature-controlled breadboard (PTC1, Thorlabs, Inc., USA) to maintain temperature at 37 °C.

Zhang F., Mo M., Jiang J., Zhou X., McBride M., Yang Y., Reilly K.S., Grys T.E., Haydel S.E., Tao N, & Wang S. (2022). Rapid Detection of Urinary Tract Infection in 10 Minutes by Tracking Multiple Phenotypic Features in a 30-Second Large Volume Scattering Video of Urine Microscopy. ACS sensors, 7(8), 2262-2272.

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Voltage-Clamp and Current-Clamp Recordings of Hippocampal Neurons

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An EPC 800 Heka Elektronik
patch-clamp amplifier was used for recording electrical activity
of hippocampal neurons that were cultured on biointerfaces. The current-clamp
recordings for transmembrane voltage and voltage-clamp recordings
for transmembrane current measurements were performed in whole-cell
configuration. No wire was connected to the biointerfaces. aCSF was
used as the extracellular medium. The patch-pipette resistance of
5–8 MΩ was used for the recordings. Patch pipettes were
filled with the intracellular medium as described above. For blocking
the voltage-gated sodium channels, 5 mM QX-314 chloride was added
into the intracellular medium. For the statistical analysis of action
potentials, the current clamp data was downsampled without causing
changes in the properties of action potentials to conduct the analysis
with a feasible computational complexity. A digital camera integrated
with the Olympus T2 upright microscope was used for monitoring the
neurons and the movement of the patch pipette. Biointerfaces were
illuminated from the bottom using M780LP1 Thorlabs LED driven by Thorlabs
DC2200 LED driver.

Karatum O., Kaleli H.N., Eren G.O., Sahin A, & Nizamoglu S. (2022). Electrical Stimulation of Neurons with Quantum Dots via Near-Infrared Light. ACS Nano, 16(5), 8233-8243.

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Dual-Channel LVSi Imaging System for Scattering Analysis

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The dual channel LVSi imaging system (Fig. 1A and Supporting Information Fig. S1) consists of two 800 mW, 780 nm infrared (IR) LEDs (M780LP1, Thorlabs, Inc., USA), each with collimating and focusing lens and a central blocking aperture to focus a ring-shaped illumination through the sample or the reference cuvettes. Wide-view and deep field depth scattering images were recorded by two CMOS camera (BFS-U3–16S2M-CS, Point Grey Research Inc., Canada) at 10 fps through two variable zoom lenses (NAVITAR 12X, Navitar, USA) with zoom factors set at 2.0X for the sample and reference cuvettes. The image volume was determined by the viewing size and focal depth of the optics. For the experiments described in this study, the viewing volume of 2.5 mm × 1.9 mm × 1.0 mm was equivalent to 4.8 μL at 2.0X magnifying power. The imaging system was enclosed in a thermally isolated housing unit with a controlled temperature (37°C).

Zhang F., Jiang J., McBride M., Yang Y., Mo M., Iriya R., Peterman J., Jing W., Grys T., Haydel S.E., Tao N, & Wang S. (2020). Direct Antimicrobial Susceptibility Testing on Clinical Urine Samples by Optical Tracking of Single Cell Division Events. Small (Weinheim an der Bergstrasse, Germany), 16(52), e2004148.

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Dual-Channel Large Volume Scattering Imaging

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The dual channel large volume scattering imaging system (Fig. 1A) consists of two 800 mW, 780 nm infrared (IR) LEDs (M780LP1, Thorlabs, Inc., USA), each with collimating and focusing lens and a central blocking aperture to focus a ring-shaped illumination through the sample or the reference cuvettes. Wide-view and deep field depth scattering images were recorded by two CMOS camera (BFS-U3–16S2M-CS, Point Grey Research Inc., Canada) at 10 fps through two variable zoom lenses (NAVITAR 12X, Navitar, USA) with zoom factors set at 2.0X for the sample and reference cuvettes. The image volume was determined by the viewing size and focal depth of the optics. For the experiments described in this study, the viewing volume of 2.5 mm × 1.9 mm × 1.0 mm was equivalent to 4.8 μL at 2.0X magnifying power. The imaging system was enclosed in a thermally-isolated housing unit with a controlled temperature (37°C).

Zhang F., Jiang J., McBride M., Zhou X., Yang Y., Mo M., Peterman J., Grys T., Haydel S.E., Tao N, & Wang S. (2021). Rapid Antimicrobial Susceptibility Testing on Clinical Urine Samples by Video-based Object Scattering Intensity Detection. Analytical chemistry, 93(18), 7011-7021.

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Fluorescence Microscopy of Liver Tissue

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The tumor-bearing livers and kidneys and hydrodynamic-transfected livers were harvested and fixed in 4% paraformaldehyde for 24 h. After paraffin embedding, coronal sections (5 µm thick) of the livers were cut. Standard hematoxylin and eosin (H&E) staining was performed on the sections, which were examined using bright-field microscopy (NanoZoomer, Hamamatsu) with a 20 × 0.67 NA objective lens.
A lab-made fluorescence imager, including a CCD camera (DV412-BV, Andor) and camera lens (SP 272E, Tamron, 90 mm, F/2.8), was used for fluorescence imaging. A laser diode (HL6738MG, Thorlabs Inc., 690 nm, 30 mW) was used for excitation, and a bandpass filter (FB750-40, Thorlabs Inc. 750 nm, FWHM = 40 ± 8 nm) was used as an emission filter. A near-infrared LED (M780LP1, Thorlabs Inc., 780 nm, 800 mW) was used to switch the DrBphP-PCM/DrSplit proteins to Pr state, and a red LED (M625L3, Thorlabs Inc., 625 nm, 700 mW) was used to switch the proteins to Pfr state.

Li L., Shemetov A.A., Baloban M., Hu P., Zhu L., Shcherbakova D.M., Zhang R., Shi J., Yao J., Wang L.V, & Verkhusha V.V. (2018). Small near-infrared photochromic protein for photoacoustic multi-contrast imaging and detection of protein interactions in vivo. Nature Communications, 9, 2734.

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Photo-electric Characterization of Interfaces

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Samples were placed on a holder, and each electrode was sequentially contacted. A platinum wire immersed in physiological saline solution (NaCl 0.9 %) was used as counter electrode. 10-ms light pulses were delivered by a 565-nm (M565L3, Thorlabs), 730-nm (M730L4, Thorlabs), or 780-nm (M780LP1, Thorlabs) LED focused at the sample level. Photo-voltage and photo-current were measured using respectively a voltage amplifier (1201, band DC-3000 Hz, DL-Instruments) and a current amplifier (1212, DL-Instruments). Data sampling (40 kHz) and instrument synchronization were obtained via a DAQ board (PCIe-6321, National Instruments) and a custom-made software. Data analysis was performed in Matlab (Mathworks). When evaluating the photo-preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this this version posted January 28, 2020. ; https://doi.org/10.1101/2020.01.27.920819 doi: bioRxiv preprint 14 voltage and photo-current densities generated by the interface, also the area of the connecting line exposed to light has been considered.

Leccardi M.J.I.A., Chenais N.A.L., Ferlauto L., Kawecki M., Zollinger E.G., & Ghezzi D. (2020). Tailored polymeric, photovoltaic, and near-infrared-responsive neuroprosthesis.

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