Pgl4.11 vector

Manufactured by Promega

Sourced in United States

The PGL4.11 vector is a plasmid used in molecular biology experiments. It provides a backbone for the insertion and expression of genes of interest in various cell types.

Automatically generated - may contain errors

Lab products found in correlation

Dual luciferase reporter assay system, by Promega (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Pcdna3.1 vector, by Thermo Fisher Scientific (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Iproof high fidelity dna polymerase, by Bio-Rad (1 mentions) In fusion hd cloning plus kit, by Takara Bio (1 mentions) Tks gflex dna polymerase, by Takara Bio (1 mentions) Luminoskan ascent instrument, by Thermo Fisher Scientific (1 mentions) Prl tk, by Promega (1 mentions)

Spelling variants of this product

PGL4 vector (65 citations) PGL4.11 [luc2P] vector (9 citations) PGL4.11-Basic Vector (2 citations)

5 protocols using pgl4.11 vector

Transcriptional Regulation of Mouse Spib and Sox8

Check if the same lab product or an alternative is used in the 5 most similar protocols

The coding sequences of mouse Spib and Sox8 were amplified from RANKL-stimulated, organoid–derived cDNA and subcloned into pcDNA3.1 vector (Life Technologies). The Gp2 promoter region (0 to –2,494 nucleotides) was amplified from genomic DNA of C57BL/6 mouse and subcloned into pCRII-Blunt-TOPO vector (Life Technologies), and then into pGL4.11 vector (Promega). PCR primers for cloning are summarized in Table S1. HEK293T and CMT93 cells were cultured in a 24-well plate and transfected with the combination of expression plasmids using Lipofectamine 3000 (Life Technologies). After 24-h incubation, luciferase activity was measured using the Dual-Luciferase Reporter Assay System (Promega).

Kimura S., Kobayashi N., Nakamura Y., Kanaya T., Takahashi D., Fujiki R., Mutoh M., Obata Y., Iwanaga T., Nakagawa T., Kato N., Sato S., Kaisho T., Ohno H, & Hase K. (2019). Sox8 is essential for M cell maturation to accelerate IgA response at the early stage after weaning in mice. The Journal of Experimental Medicine, 216(4), 831-846.

+ Open protocol

+ Expand

Cloning and Bioinformatic Analysis of Periostin Promoter

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the reporter constructs, the periostin promoter regions (−4000 to +50 bp) were amplified from human genomic DNA (Zyagen, CA) by PCR using iProof High-Fidelity DNA Polymerase (Bio-Rad, CA). The PCR products were subcloned into the pGL4.11 vector (Promega, WI) upstream of a luciferase gene using the KpnI/EcoRV restriction sites. Computational analysis to identify promoter-bound transcription factors was done using the Transcription Element Search System (TESS) (http://www.cbil.upenn.edu/cgi-bin/tess/tess).

Amara S., Lopez K., Banan B., Brown S.K., Whalen M., Myles E., Ivy M.T., Johnson T., Schey K.L, & Tiriveedhi V. (2014). Synergistic effect of pro-inflammatory TNFα and IL-17 in periostin mediated collagen deposition: Potential role in liver fibrosis. Molecular immunology, 64(1), 26-35.

+ Open protocol

+ Expand

BCL2 Promoter Cloning and Deletion

Check if the same lab product or an alternative is used in the 5 most similar protocols

A DNA fragment of human BCL2 promoter (–1670/+16) was PCR amplified and cloned into the pGL4.11 vector (Promega). Deletion of the predicted KLF-binding site (–1125/–1099) within the cloned BCL2 promoter fragment (–1670/+16) was performed using the In-Fusion HD Cloning Plus Kit (TaKara Bio, 638910). All PCR products were validated by DNA-Seq.

Zhao G., Zhao Y., Lu H., Chang Z., Liu H., Wang H., Liang W., Liu Y., Zhu T., Rom O., Guo Y., Chang L., Yang B., Garcia-Barrio M.T., Lin J.D., Chen Y.E, & Zhang J. (2022). BAF60c prevents abdominal aortic aneurysm formation through epigenetic control of vascular smooth muscle cell homeostasis. The Journal of Clinical Investigation, 132(21), e158309.

+ Open protocol

+ Expand

Constructing Luciferase Reporter Vectors with Diverse Regulatory Elements

Check if the same lab product or an alternative is used in the 5 most similar protocols

To construct p4xCRE-3xSRE-TATA-Luc2P, four tandem repeats of the cAMP-response element (5′-AGCCTGACGTCAGAG-3′)^{42 (link),43 (link)}, three tandem repeats of the serum-response element (5′-AGGATGTCCATATTAGGACATCT-3′)^{44 (link),45 (link)} derived from the human c-fos gene, and an adenovirus E1b TATA sequence (5′-AGGGTATATAATG-3′)^{46 (link),47 (link)} were subsequently inserted into a pGL4.11 vector (Promega, Madison, WI, USA). Similarly, p4xCRE-TATA-Luc2P and p3xSRE-TATA-Luc2P were constructed. To construct p2xSRF-TATA-Luc2P, two tandem repeats of serum-response factor-response element (5′-TCGACTGTACTGTATGTCCATATTAGGACATCTG-3′)^{48 (link),49 (link)} derived from the human c-fos gene and the TATA sequence were inserted into a pGL4.11 vector. The NFAT luciferase reporter vector was obtained from Addgene (#10959)^{50 (link)}. To construct the GPCR expression vectors, we utilized the Gateway system and subcloned 258 full-length human GPCR cDNAs from pDONR201 vectors^{51 (link)} into pcDNA3.2/V5-DEST vectors (Thermo Fisher Scientific). Mutant GPR55 expression vectors were constructed by site-directed mutagenesis with Tks Gflex DNA polymerase (Takara Bio, Shiga, Japan) using primers (Supplementary Table 1).

Harada N., Okuyama M., Teraoka Y., Arahori Y., Shinmori Y., Horiuchi H., Luis P.B., Joseph A.I., Kitakaze T., Matsumura S., Hira T., Yamamoto N., Iuni T., Goshima N., Schneider C., Inui H, & Yamaji R. (2022). Identification of G protein-coupled receptor 55 (GPR55) as a target of curcumin. NPJ Science of Food, 6, 4.

+ Open protocol

+ Expand

Promoter Activity Assay of TRF, RAD50, VEGF

Check if the same lab product or an alternative is used in the 5 most similar protocols

The promoter regions of TRF1, TRF2, RAD50, and VEGF (1 000 bp upstream from the transcription start site (TSS)) in Supplementary Table S2 were synthesized (Tsingke Biotechnology, China) and constructed into the pGL4.11 vector (Promega, USA, Cat. No. E6661). The pGL4.11 constructs (150 ng) and internal control vector pRL-TK (30 ng, Promega, USA, Cat. No. E2241) were co-transfected into mouse fibroblasts using Lipofectamine 2000 (Thermo Fisher, USA). At 48 h post transfection, luminescence was measured using a Dual-Luciferase Reporter Assay System (Promega, USA, Cat. No. E1960) and Luminoskan Ascent instrument (Thermo Fisher, USA).

Li K.Q., Liu G.J., Liu X.Y., Chen Q.F., Huang X.Y., Tu Q., Zhang J., Chang Q., Xie Y.H., Hua R., Xu D.M., Liu Z, & Zhao B. (2023). EPAS1 prevents telomeric damage-induced senescence by enhancing transcription of TRF1, TRF2, and RAD50. Zoological Research, 44(3), 636-649.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!