Tiangen rna preparation kits

Manufactured by Tiangen Biotech

Sourced in China

Tiangen RNA preparation kits are a series of laboratory products designed for the isolation and purification of RNA from various biological samples. The kits utilize advanced extraction and purification techniques to obtain high-quality RNA for downstream applications such as gene expression analysis, reverse transcription, and other molecular biology procedures.

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Lab products found in correlation

Agilent 2100, by Agilent Technologies (1 mentions) Agilent 2100 bioanalyzer, by Agilent Technologies (1 mentions) Nanodrop nd 1000 spectrophotometer, by Thermo Fisher Scientific (1 mentions) 2100 bioanalyzer, by Agilent Technologies (1 mentions)

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RNA preparation kit RNA kits

4 protocols using tiangen rna preparation kits

Strawberry Transcriptome Analysis Pipeline

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Strawberry plants were grown in the plastic greenhouse at Jiangsu Academy of Agricultural Sciences. The fruit receptacles were frozen in liquid nitrogen immediately and stored at −80 °C after being cut into small pieces. The other tissues were directly frozen in liquid nitrogen and stored at −80 °C until further use.
Total RNA was extracted using Tiangen RNA preparation kits (Tiangen Biotech, Beijing, China) following the provided protocol. RNA integrity was assessed using a Nanodrop ND-1000 spectrophotometer and 2100 Bioanalyzer. Equal amounts of RNA from different tissues (mature leaf, flower, dwarf stem, root, and receptacle tissues, from six different stages) were combined for SMRT sequencing, and the total RNA from red fruit of two strawberry varieties (Xiaobai and Benihoppe) was used for Illumina sequencing.

Yuan H., Yu H., Huang T., Shen X., Xia J., Pang F., Wang J, & Zhao M. (2019). The complexity of the Fragaria x ananassa (octoploid) transcriptome by single-molecule long-read sequencing. Horticulture Research, 6, 46.

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RNA Extraction and Sequencing Protocols

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Total RNAs were extracted by the Tiangen RNA preparation kits (Tiangen Biotech, Beijing, China) following the manufacturer’s protocol. We used the following methods to test for the quality of the RNA. First, RNA degradation was estimated on agarose gels. Second, RNA purity (OD260/280), concentration and absorption peaks were determined using Nanodrop. Third, the integrity was determined using Agilent 2100. The detection indexes included: RIN value, 28S/18S, baseline for spectra and 5S peak (Table S4). RNA samples were used for constructing cDNA libraries. One microgram for each RNA sample from the two temperature treatments was pooled together at an equal ratio and used for PacBio single-molecule long-read sequencing. All six samples (two temperature treatment, three biological replicates) were used for Illumina sequencing (Fig. S5).

Chen S., Qiu G, & Yang M. (2019). SMRT sequencing of full-length transcriptome of seagrasses Zostera japonica. Scientific Reports, 9, 14537.

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Transcriptome Profiling of Aquatic Organisms

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Nine samples (three tissues-hepatopancreas, gill, and muscle, each with three biological replicates) were quickly separated on ice. Total RNA was extracted using Tiangen RNA preparation kits (Tiangen Biotech, Beijing, China) following the manufacturer’s protocol. The following methods were used to test the RNA quality. First, the extent of RNA degradation was estimated using agarose gel electrophoresis. Second, RNA purity (OD260/280), concentration, and absorption peaks were determined using a Nanodrop spectrophotometer. Third, RNA integrity was determined using Agilent 2,100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, United States). Detection indexes included the RIN value, 28S/18S, baseline for spectra, and 5S peak. RNA samples were used to construct cDNA libraries. One microgram of each RNA sample from all samples was added together in a 1:1 ratio and used for PacBio single-molecule long-read sequencing. All nine samples were used for Illumina sequencing.

Liao X., Liu Y., Han T., Yang M., Liu W., Wang Y., He C, & Lu Z. (2022). Full-Length Transcriptome Sequencing Reveals Tissue-Specific Gene Expression Profile of Mangrove Clam Geloina erosa. Frontiers in Physiology, 13, 851957.

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Tissue-Specific Transcriptome Profiling of Sweet Potato

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Xushu18, one of the most widely cultivated I. batatas varieties in China, was selected for transcriptome sequencing in this study. Eight tissues of young leaves, mature leaves, apical shoots, mature stems, fibrous roots, initiating tuberous roots, expanding tuberous roots, and mature tuberous roots from one individual were collected and pooled together in approximately equivalent weights (Figs. 1A–1H). Similarly, tissues of young leaves, mature leaves, shoots, stems, and roots of a diploid I. trifida plant were collected and pooled. Collected samples were frozen in liquid nitrogen immediately after collection and stored at −80 °C until use.
Total RNAs were extracted using Tiangen RNA preparation kits (Tiangen Biotech, Beijing, China) following the provided protocol. RNA quality and quantity were determined using a Nanodrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) and a 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA). Qualified RNA samples were subsequently used in constructing PacBio cDNA or RNA-seq libraries.

Ding N., Cui H., Miao Y., Tang J., Cao Q, & Luo Y. (2019). Single-molecule real-time sequencing identifies massive full-length cDNAs and alternative-splicing events that facilitate comparative and functional genomics study in the hexaploid crop sweet potato. PeerJ, 7, e7933.

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