Oleic acid (90%) and iron chloride tetrahydrate (98%) were purchased from Alpha Aesar. 1-Pentanol (≥99%), cetrimonium bromide (CTAB, 98%), chloroform (99.4%), dimercaptosuccinic acid (DMSA, 90%), ethanol (99.8%), iron nitrate nonahydrate (≥98%), n-hexane (≥97%), sodium hydroxide (98%), toluene (99.7%), and triethylamine (TEA, >99.5%) were purchased from Sigma Aldrich. Dimethyl sulfoxide (DMSO, ≥99.5%) was purchased from Sigma. Poloxamer 188 (P188) and sodium hydroxide (1 mol L−1) were purchased from Panreac. 2-[Methoxy(polyethyleneoxy)6–9propyl] trimethoxysilane (PEGTMS, 90%) was purchased from ABCR GmBH. Stearic acid (SA, 97%) was purchased from Acros Organics.
Dimercaptosuccinic acid
Manufactured by Merck Group
Sourced in Spain, Pakistan
Dimercaptosuccinic acid is a chelating agent used in analytical chemistry and materials science applications. It is a dithiol compound with the chemical formula C4H6O4S2.
Automatically generated - may contain errors
Lab products found in correlation
Ethanol, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Sodium bicarbonate, by Merck Group (2 mentions)
Milli q advantage a10 system, by Merck Group (2 mentions)
N hexane, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Oleic acid, by Thermo Fisher Scientific (1 mentions)
Toluene, by Merck Group (1 mentions)
Stearic acid, by Thermo Fisher Scientific (1 mentions)
Triethylamine, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by ITW Reagents (1 mentions)
Iron chloride tetrahydrate, by Thermo Fisher Scientific (1 mentions)
Cetrimonium bromide, by Merck Group (1 mentions)
Iron nitrate nonahydrate, by Merck Group (1 mentions)
1 pentanol, by Merck Group (1 mentions)
Nitric acid, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Fecl3 6h2o, by Merck Group (1 mentions)
6 protocols using dimercaptosuccinic acid
1
Synthesis and Characterization of Magnetic Nanoparticles
2
Synthesis of Metal Nanoparticles
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Iron(III) chloride hexahydrate
(FeCl3·6H2O, 97%), copper(II) chloride
dihydrate (CuCl2·2H2O, <99%), sodium
acetate anhydrous (CH3COONa), poly(vinylpyrrolidone) (PVP
K30, MW 4000 Da), ethylene glycol (EG)
(99.8%), sulfur powder (S, ≥99%), poly(ethylene glycol) (PEG,
wt 8000) (99.8%), ethanol (CH3CH2OH, 96%), glutathione
(GSH, <98%), glutathione disulfide (GSSG, <98%), ethylenediaminetetraacetic
acid disodium salt dihydrate (EDTA 99%), tris(hydroxymethyl)aminomethane
(TRIS, ≥99.8%), 3,3′,5,5′-tetramethylbenzidine
(TMB, ≥98%), hydrogen peroxide (H2O2,
33% v/v), dimethyl sulfoxide (DMSO, ≥99.9%), sodium bicarbonate
(NaHCO3, 99%), hydrochloric acid (HCl, 37%), nitric acid
(HNO3, 65%), acetonitrile (anhydrous, ACN, ≥99.9%),
dimercaptosuccinic acid (DMSA, 99.0%), and phosphate-buffered saline
solution (PBS) were purchased from Sigma-Aldrich. Deionized water
was obtained from a Milli-Q Advantage A10 System with a resistivity
of 18.2 MΩ·cm (Merk Millipore, Germany). All chemicals
were used without any further purification.
(FeCl3·6H2O, 97%), copper(II) chloride
dihydrate (CuCl2·2H2O, <99%), sodium
acetate anhydrous (CH3COONa), poly(vinylpyrrolidone) (PVP
K30, MW 4000 Da), ethylene glycol (EG)
(99.8%), sulfur powder (S, ≥99%), poly(ethylene glycol) (PEG,
wt 8000) (99.8%), ethanol (CH3CH2OH, 96%), glutathione
(GSH, <98%), glutathione disulfide (GSSG, <98%), ethylenediaminetetraacetic
acid disodium salt dihydrate (EDTA 99%), tris(hydroxymethyl)aminomethane
(TRIS, ≥99.8%), 3,3′,5,5′-tetramethylbenzidine
(TMB, ≥98%), hydrogen peroxide (H2O2,
33% v/v), dimethyl sulfoxide (DMSO, ≥99.9%), sodium bicarbonate
(NaHCO3, 99%), hydrochloric acid (HCl, 37%), nitric acid
(HNO3, 65%), acetonitrile (anhydrous, ACN, ≥99.9%),
dimercaptosuccinic acid (DMSA, 99.0%), and phosphate-buffered saline
solution (PBS) were purchased from Sigma-Aldrich. Deionized water
was obtained from a Milli-Q Advantage A10 System with a resistivity
of 18.2 MΩ·cm (Merk Millipore, Germany). All chemicals
were used without any further purification.
3
Synthesis and Characterization of Iron Oxide Nanoparticles
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Sodium oleate 82%, FeCl3·6H2O 97%, oleic acid 90%, octadecene 90%, dimercaptosuccinic acid 98% (DMSA), dimethyl sulfoxide ≥99.9% (DMSO), acetic acid, and 12 kDa cellulose membrane were purchased from Sigma-Aldrich (Madrid, Spain). Absolute ethanol 99.5%, hexane 95% and toluene 99.5% were purchased from PanReac (Barcelona, Spain). All other chemicals (NaOH, HNO3 65%, HCl 37%, NaCl, MgCl2, etc.) were of the highest quality commercially available and used as received. Milli-Q water (resistivity 18.2 MΩ·cm at 25 °C) was used in all experiments. Type-I collagen powders extracted from rat tails (N_COL) were provided by NOVAICOS Srl (Novara, Italy, https://www.novaicos.com/ (accessed on 20 December 2021)).
4
Synthesis of Water-Soluble CdSe Nanocrystals
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Water-soluble CdSe core NCs were obtained using mercaptosuccinic acid as a surface-modifying agent. Briefly, 20–30 mg of NCs was dissolved in chloroform, after which 3 mL of dimercaptosuccinic acid (Sigma, dissolved in 150 mM PBS, pH 7.3) was added to 6 mL of NCs in chloroform. Water containing water-soluble CdSe NCs was carefully decanted and subjected to ultrafiltration to remove free (nonreacted) dimercaptosuccinic acid. The ultrafiltration was carried out using a concentrator (Vivaspin-6/20, Sartorius AG, Gottingen, Germany).
5
Synthesis and Characterization of Copper-Iron Nanoparticles
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Iron(iii ) chloride hexahydrate (FeCl3·6H2O, 97%), copper(ii ) chloride dihydrate (CuCl2·2H2O, ≥99.0%), sodium acetate anhydrous (CH3COONa, 99.0%), bovine serum albumin (BSA), ethylene glycol (EG), dimercaptosuccinic acid (DMSA, 99.0%), glutathione, glutathione oxidized (GSSG, ≥98.0% HPLC), 5,5′-dithiobis(2-nitrobenzoic acid) (DNTB), hydrogen peroxide (H2O2, 33% v/v), sodium bicarbonate (NaHCO3, 99%), methylene blue (MB, ≥95.0), dihydroethidium (DHE, 99%), potassium dioxide (KO2, >99%), trichloroacetic acid (CCl3COOH, >99%) and tris(hydroxymethyl)aminomethane (TRIS, >99%) were purchased from Sigma Aldrich. Acetonitrile (ACN) was purchased from WVR (Avantor). UPLC grade water was obtained from a Milli-Q Advantage A10 System with resistivity of 18.2 mΩ (Merk Millipore, Germany). Phalloidin-Alexa 488 was acquired from Molecular Probes (A12379) and Fluoromount-G + DAPI from EMS (17984-24). Dulbecco's modified Eagle medium (DMEM) was purchased from Lonza (Ref. 12-614F). Dulbecco's phosphate buffered saline (DPBS 1×, Ref. 17-512F) and PBS (Ref. BE02-017F) were also purchased from Lonza.
6
Synthesis and Characterization of Multifunctional Nanoparticles
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Sebacic acid (99% purity), glycerol (99% purity), polyethylene glycol (400 Dalton), sodium hydroxide, acetone, span 20, tween 80, calcium chloride (CaCl2), Di-mercapto succinic acid (DMSO) and ethanol (99% purity) were purchased from Sigma Aldrich (Karachi, Pakistan). Ferric chloride hexahydrate (FeCl3·6H2O) and ferrous chloride tetrahydrate (FeCl2·4H2O) were purchased from DUKSAN (Gyeonggi-do, South Korea). Perfluoropentane was purchased from Shanghai Tianfu (Shanghai, China). PBS tablets were purchased from Oxoid (Basingstoke, UK). The thrombin reagent was purchased from Roche Pharma (Karachi, Pakistan). Gadovist was purchased from Bayer’s Pharmaceuticals (Karachi, Pakistan). Sonovue® (Bracco Int. High Wycombe, UK) was purchased from Shifa International Hospital Pharmacy (Islamabad, Pakistan). The ELISA kit was purchased by Nanjing Pars Biochem Ltd. (Nanjing, China) The kit contained standards, antibody-coated microtiter plate, chromogen A, chromogen B, HRP conjugate and stop solution.
Blood was collected from healthy donors in citrated and EDTA-containing vials. Vials and Aspirin (High noon Laboratories Ltd., Lahore, Pakistan) were purchased from a local pharmacy. LPS extracted from E. coli was a gift from the National Institute of Health, Islamabad, Pakistan. MCF-7 cell lines (HTB-22™) were purchased from ATCC (Manassas, VA, USA).
Blood was collected from healthy donors in citrated and EDTA-containing vials. Vials and Aspirin (High noon Laboratories Ltd., Lahore, Pakistan) were purchased from a local pharmacy. LPS extracted from E. coli was a gift from the National Institute of Health, Islamabad, Pakistan. MCF-7 cell lines (HTB-22™) were purchased from ATCC (Manassas, VA, USA).
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