Samples of wort and beer (3 mL) were placed in sealed pressure vessels, with the addition of nitric acid (5 mL, 68%), and subjected to wet mineralisation in a Mars Xpress microwave oven (1200 W, 170 °C, 15 min) (CEM Corp., Matthews, NC, USA). The samples were then diluted with deionised water and their absorbance at 202.6 nm for Mg2+ and 213.9 nm for Zn2+ was determined by atomic absorption spectrometry with a flame atomization technique (Varian AA240FS), using an automatic dispensing sample system (SIPS-20, Agilent, Santa Clara, CA, USA). Gas flow was 3.5 dm3/min (acetylene) and air was 14 dm3/min. standard solutions (Mg2+, Zn2+–respectively 100 and 5.0 mg/L) were prepared from 1000 mg/L standard solution (Merck, Bilerica, MA, USA).
Sips 20
Manufactured by Agilent Technologies
Sourced in United States
The SIPS-20 is a digital sequential injection sampling system designed for analytical instruments. It provides precise and accurate sample introduction and handling capabilities for various analytical techniques.
Automatically generated - may contain errors
Lab products found in correlation
Aa240fs, by Agilent Technologies (2 mentions)
Standard solution, by Merck Group (1 mentions)
Specol 11, by Zeiss (1 mentions)
Multi element standard analytical solutions, by Merck Group (1 mentions)
Hcl lamps, by Agilent Technologies (1 mentions)
Spectraa 220fs, by Agilent Technologies (1 mentions)
Milli q water, by Merck Group (1 mentions)
5 protocols using sips 20
1
Quantifying Mg and Zn in Wort and Beer
2
Calcium Quantification in Milk Matrices
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The milk matrices were rehydrated in 100 mL of water (100 g.L−1) and then filtered in a mineral cartridge filter (Macrosep 10 K, Pall Biotech, Saint-Germain-en-Laye, France). After filtration, the suspension was centrifuged at a temperature of −20 °C and at 5000× g for 90 min in order to collect at least 5 g of filtrate. Calcium concentration levels were then estimated by atomic absorption spectrophotometry. Briefly, a solution of lanthanum (25 g.L−1); HCl (1% in H2O); and standard solutions of calcium, sodium, potassium, and magnesium; and the milk matrices were injected into a dual sample introduction pump system (SIPS 20, Agilent Technologies, Les Ulis, France). After setting up the spectrophotometer (VARIAN AA 240FS, Agilent Technologies), the emission intensity was measured for all the standards and samples with the software spectra 5.1 PRO. The calcium concentrations in the samples were determined according to a calibration curve reflecting absorbance vs. calcium, magnesium, and potassium concentrations. Calcium concentration levels were then evaluated in the oral, gastric, and intestinal phases according to dilution factors.
3
Determination of Metals in Buckwheat Wort
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The contents of metals in the samples of buckwheat wort were determined by atomic absorption spectrometry with the flame atomization technique (Varian AA240FS), using an automatic dispensing sample system (SIPS–20) (Agilent, Santa Clara, CA, USA). The flows of gas (acetylene) and air were 3.5 and 14 L/min, respectively. Before analysis, the samples were subjected to a process of wet mineralization with the addition of 4 mL of concentrated HNO3, in sealed pressure vessels using a microwave oven Mars Xpress (1200 W, 170 °C, 15 min; CEM Corp., Matthews, NC, USA). The elements were determined using a single sample aspiration via a rapid sequence mode (called Fast Sequential). Standard solutions of cations were prepared according to MERCK standards (1000 mg·L−1) (Merck, Bilerica, MA, USA).
4
Nutritional Composition Analysis of Animal Feeds
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The chemical composition of feed compounds and complete diets was determined according to the standard methods AOAC (2005): the nitrogen by the Kjeldahl method (984.13) using Kjeltec 2300 Foss Tecator apparatus (Hoganas, Sweden), crude protein by multiplying N-content by 6.25, crude fat by ether extraction (920.39), and crude fiber by the Hennenberg–Stohmann method (978.10) using Fibertec Tecator (Sweden) apparatus. Phosphorus was analyzed after mineralization with nitric acid (HNO3) and perchloric acid (HClO4) by the ammonium vanadomolybdate method using a spectrophotometer (Specol 11) (Carl Zeiss, Jena) at 470 nm (995.11). Calcium and other mineral (important for the dipeptide synthesis and metabolism) content were determined by the atomic absorption spectrophotometry using AA 240 FS apparatus with SIPS 20 (Varian) (968.08).
5
Quantifying Iron Levels in Tissues
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Collected uterine and testicular tissues, and hair samples were handled using plastic instruments with special care taken to avoid any contamination. Thawed tissues were dried in an oven at 40 o C, flushed twice with MilliQ water (Millipore, USA) to ensure the removal of blood remnants and dried again to a constant weight. All samples (hair, uterine and testicular tissues) were subjected to a complete digestion performed with suprapur 14 mol/L HNO 3 (Sigma-Aldrich, Germany) in sealed plastic tubes using an oven (80 o C). The concentration of iron in the investigated samples was determined by the fast sequential atomic absorption spectrometer SpectrAA 220 FS (Varian, Australia) equipped with HCL lamps (Varian, Australia) and a Sampling System with an Electronic Control Module SIPS-20 (Varian, Australia). Following optical conditions were applied: wavelength 248.3 nm, slit 0.2 nm, with background correction from a deuterium lamp. The calibration was performed using multi-element standard analytical solutions (Merck, Germany). A control without any tissue but containing HNO 3 was performed in order to exclude the interference of any procedural step on metal content determination -the iron content was below the limit of detection. The final results were given as ppm of Fe (mg Fe kg -1 sample).
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