A Thorlabs Bergamo 2-photon microscope with SpectraPhysics InsightX3 dual beam laser was used to record calcium dynamics. GCaMP7f signals in corticospinal neurons were recorded at 940 nm using a 16X water-immersion objective (Olympus, WD 3.0 NA 0.8, D32 diameter) in a single imaging plane at depth 650 to 750 mm. All images were acquired at a rate of approximately 30 Hz per frame of 515 ×512 pixels using ThorImageLS 3.2 software with an 8 kHz galvo/resonant scanner speed. ThorSync 3.2 software was used in synchrony to store the frame times, triggers and signals coming into and out of the microscope for an off-line data analysis.
Bergamo 2 photon microscope
Manufactured by Thorlabs
The Bergamo 2-photon microscope is a high-performance imaging system designed for advanced biological research. It utilizes two-photon excitation microscopy to enable deep tissue imaging with minimal photodamage. The microscope features a high-power ultrafast laser, optimized optics, and sensitive detectors to provide high-resolution, three-dimensional imaging capabilities.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using bergamo 2 photon microscope
1
Calcium Dynamics in Corticospinal Neurons
2
Two-Photon Microscopy of Brain States
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Animals were imaged on a customized Thorlabs Bergamo 2-photon microscope equipped with the state monitoring described above. Signals from state monitoring and microscope signals were aligned using TTL pulses acquired on an Intan RHD system.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!