Automate express forensic dna extraction system

Manufactured by Thermo Fisher Scientific

Sourced in United States

The AutoMate Express Forensic DNA Extraction System is a laboratory instrument designed for the automated extraction of DNA from forensic samples. The system uses magnetic bead technology to isolate and purify DNA, enabling efficient and standardized processing of multiple samples simultaneously.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

AutoMate Express DNA extraction system (2 citations)

9 protocols using automate express forensic dna extraction system

Optimized DNA Extraction and STR Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA was extracted using The AutoMate ExpressTM Forensic DNA Extraction System with the PrepFilerTM Express BTA kit (Thermo Fisher Scientific, Darmstadt, Germany) to a 50-μL elution volume according to manufacturer’s instructions. Fluorometric DNA quantitation was performed using the Quantus™ Fluorometer with the QuantiFluor® dsDNA System (Promega, Walldorf, Germany) according to manufacturer’s instructions.
Additionally, triplicates of a human clavicle were processed according to the pig bone samples and stored for 10, 20, and 30 days and 35 °C in the substrate shown to result in the highest DNA recovery in pig bone. To check for STR profile quality, DNA from the positive control as well as the stored samples was amplified using the PowerPlex® ESX 17 (Promega, Mannheim, Germany) and the GlobalFiler™ PCR kits (Thermo Fisher Scientific, Darmstadt, Germany) according to manufacturer’s recommendations. The samples were analyzed using the 3130 Genetic Analyzer with the GeneMapper® ID software by Thermo Fisher Scientific.

de Arellano Sánchez J.A., Ortiz J.M., Quintero A.L., Pfeiffer H., Vennemann M, & Bauer H. (2022). Comparing preservation substrates under field conditions for efficient DNA recovery in bone. International Journal of Legal Medicine, 137(2), 297-301.

+ Open protocol

+ Expand

Oral Swab DNA Extraction and Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA was extracted from the oral swab samples of 50 individuals (men:42 individuals, women:8 individuals). All the individuals provided informed consent. Oral swabs were collected using clean DNA-free cotton swabs. The DNA was extracted using the PrepFiler Express TM Forensic DNA Extraction Kit (Thermo Fisher Scientific) and AutoMate Express TM Forensic DNA Extraction System (Thermo Fisher Scientific). The extract was quantified using the human genome quantification kit ver. 2 (TaKaRa) by the real-time PCR method using the 207 bp DNA sequence present at the D17Z1 locus as an index.

Momota F., Tsuji A., Ishiko A, & Ikeda N. (2021). Examination of the usefulness of next-generation sequencing in mixed DNA samples. Legal medicine (Tokyo, Japan), 51.

+ Open protocol

+ Expand

DNA Extraction and Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

In this study, three male DNA controls were used, including M308 (Bejing Microread Genetics, China), DNA007 (Thermo Fisher Scientific, USA), and 9948 (Thermo Fisher Scientific), whereas for female DNA control, an in‐house sample was used.
For noncontrol DNA samples, buccal swabs from in‐house staff were collected with informed consent. The DNA samples were extracted using the PrepFiler Express kit and the AutoMate Express Forensic DNA Extraction System (Thermo Fisher Scientific) and quantified using the Quantifiler Trio DNA Quantification Kit (Thermo Fisher Scientific) using the QuantStudio 5 Real‐Time PCR System (Thermo Fisher Scientific). All DNA samples were collected at the Dubai Health Authority laboratory voluntarily with an informed consent under ethical approval no. DSREC‐SR‐08/2018‐03.

Albastaki A., Naji M., Lootah R., Almheiri R., Almulla H., Alreyami A., Almarri I., Yu Z., Zhang Y., Shu C, & Alghafri R. (2022). Design and development of novel single multiplex system incorporating 26 rapidly mutating Y‐STRs; 26 RM Yplex. Electrophoresis, 43(18-19), 1911-1919.

+ Open protocol

+ Expand

Nonhuman DNA Extraction and Amplification

Check if the same lab product or an alternative is used in the 5 most similar protocols

The specificity of the system was tested using DNA samples from human female and several animal species. Nonhuman samples were provided by Dubai Zoo as per Dubai Municipality authorization. DNA was extracted from the blood samples of Chimpanzees, male Gibbons and male Orang‐utans using the AutoMate Express Forensic DNA Extraction System (Thermo Fisher Scientific) according to the manufacture's protocol in the PrepFiler Express kit. After quantification (NanoDrop 2000/2000c,Thermo Fisher Scientific), 5 ng DNA was used in amplification.
A positive control test was run using DNA control standard 007 for confirmation.

+ Open protocol

+ Expand

Genomic DNA Extraction and Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

A total of 95 samples were collected (54 males and 41 females) from the central Indian population. The blood samples were collected from the participants after obtaining written informed consent. The peripheral liquid blood samples collected in K 2 EDTA vials were stored at 4 °C until further use. Genomic DNA was extracted from the blood samples by following recommended protocol of the manufacturer using AutoMate Express™ Forensic DNA Extraction System (Thermo Scientific, USA) and PrepFiler Express™ Forensic DNA Extraction Kit (Thermo Scientific, USA). Extracted genomic DNA was subjected to quantification using QuantStudio™ 5 Real-Time PCR System (Thermo Scientific, USA) using Quantifiler® Trio DNA Quantification Kit (Thermo Scientific, USA). Further, the DNA samples were normalized to 1.0 ng/µl in TE buffer and stored at -20 °C until further use.

Dash H.R., Avila E., Jena S.R., Kaitholia K., Agarwal R., Alho C.S., Srivastava A, & Singh A.K. (2022). Forensic characterization of 124 SNPs in the central Indian population using precision ID Identity Panel through next-generation sequencing. International journal of legal medicine, 136(2).

+ Open protocol

+ Expand

Rapid Swab-based DNA Extraction Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples were collected using a Copan 150C Cotton swab (Copan, Brescia, Italy) moistened with 100 μL of sterile distilled water applied using a plastic spray bottle technique (developed in Dubai police forensic DNA lab; every single spray contains approximately 50 μL). Full swab heads were used for extraction immediately after collection using the PrepFiler Express BTA™ kit with AutoMate Express Forensic DNA Extraction System according to the manufacturer's instructions (Thermo Fisher Scientific) and the final extracted sample elution was 50 μL.

Alketbi S.K. (2020). Collection of Touch DNA from Rotten Banana Skin. International Journal of Forensic Sciences, 5(4).

+ Open protocol

+ Expand

Automated DNA Extraction and Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Genomic DNA was extracted from all samples using the automated platform, Automate Express Forensic DNA Extraction System (Thermo Fisher Scientific Inc., Waltham, USA) with the Prepfiler Automated Forensic DNA Extraction System (Thermo Fisher Scientific Inc.). DNA extracts were quantified using the Quantifiler Trio Human DNA Quantification Kit (Thermo Fisher Scientific Inc.) on a 7500 Real-Time PCR System (Thermo Fisher Scientific Inc.). All procedures followed the manufacturers instructions, with elution volumes of 50 mL. A sample was considered not degraded/inhibited when the degradation index (DI) <1.5 and no IPCCT flag was triggered in Quantifiler Trio assay.

Avila E., Cavalheiro C.P., Felkl A.B., Graebin P., Kahmann A, & Alho C.S. (2019). Brazilian forensic casework analysis through MPS applications: Statistical weight-of-evidence and biological nature of criminal samples as an influence factor in quality metrics. Forensic science international, 303.

+ Open protocol

+ Expand

Automated Forensic DNA Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total DNA was extracted using the automated platform Automate Express Forensic DNA Extraction System (Thermo Fisher Scientific Inc.) with the Prepfiler Automated Forensic DNA Extraction System (Thermo Fisher Scientific Inc.), or manually via the DNA IQ System (Promega Corp., Madison, WI, USA). Autosomal DNA was quantified with the Quantifiler Human DNA Quantification Kit (Thermo Fisher Scientific Inc.) on a 7500 Real-Time PCR System (Thermo Fisher Scientific Inc.). MtDNA quantities were estimated using a 1:100 genomic to mitochondrial DNA copies ratio according to the product manual. All procedures followed the manufacturer´s instructions.

Avila E., Graebin P., Chemale G., Freitas J., Kahmann A, & Alho C.S. (2019). Full mtDNA genome sequencing of Brazilian admixed populations: A forensic-focused evaluation of a MPS application as an alternative to Sanger sequencing methods. Forensic science international. Genetics, 42.

+ Open protocol

+ Expand

Brazilian Population Genomic DNA Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols

Oral swabs or peripheral blood were obtained from 432 unrelated volunteer donors from all five different Brazilian geopolitical regions. Male contributors were preferred, in order to assure a significant sample size for investigation of SNPs located in the Y chromosome included in this study. Number of samples obtained from each Brazilian region and its States are presented in Supplementary Figure S1. Genomic DNA was extracted using the automated platform Automate Express Forensic DNA Extraction System (Thermo Fischer Scientific Inc.) with the Prepfiler Automated Forensic DNA Extraction System (Thermo Fischer Scientific Inc.), or manually using DNA IQ System (Promega Corp., Madison, WI, USA). DNA was quantified with the Quantifiler Human DNA Quantification Kit (Thermo Fischer Scientific Inc.) on a 7500 Real-Time PCR System (Thermo Fischer Scientific Inc.) or using Qubit 2.0 Fluorometer with Qubit dsDNA HS Assay Kit (Thermo Fischer Scientific Inc.). All procedures followed manu-facturer´s instructions.

Avila E., Felkl A.B., Graebin P., Nunes C.P, & Alho C.S. (2019). Forensic characterization of Brazilian regional populations through massive parallel sequencing of 124 SNPs included in HID ion Ampliseq Identity Panel. Forensic science international. Genetics, 40.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!