Log In Sign up
The largest database of trusted experimental protocols

Ubiquitin

Manufactured by Jackson ImmunoResearch
Visit Supplier
Sourced in Panama

Ubiquitin is a small protein found in eukaryotic cells. Its primary function is to tag other proteins for degradation or recycling by the proteasome. Ubiquitin attaches to target proteins through a series of enzymatic reactions, marking them for removal from the cell.

Automatically generated - may contain errors

Lab products found in correlation

Ubiquitin, by Merck Group (9 mentions) 400 fluorescent microscope, by Nikon (8 mentions) Dapi nuclear stain, by Thermo Fisher Scientific (8 mentions) Alexa fluor 594 donkey anti mouse, by Jackson ImmunoResearch (5 mentions) Morphometric system, by Nikon (5 mentions) Alexa fluor 488, by Jackson ImmunoResearch (4 mentions) Alexa fluor 594, by Jackson ImmunoResearch (3 mentions) Alexa fluor 488 donkey anti rabbit, by Jackson ImmunoResearch (3 mentions) Donkey anti mouse alexa fluor 488, by Jackson ImmunoResearch (2 mentions) Stat2, by Abcam (1 mentions) Bcl2 associated x (bax), by Jackson ImmunoResearch (1 mentions) Bcl2 associated x (bax), by Enzo Life Sciences (1 mentions) Bcl2 associated x (bax), by Abcam (1 mentions) Ubiquitin, by Thermo Fisher Scientific (1 mentions) Cxcr4, by Abcam (1 mentions) Cxcr4, by Jackson ImmunoResearch (1 mentions)

9 protocols using ubiquitin

1

Quantitative Immunofluorescence Protein Analysis

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Formalin fixed, paraffin embedded tissue slides were immunohistochemically double stained for TEC, PAK6, GNG2, STAT2, SRC (Abcam Inc., Cambridge, MA), GNA15 (MyBioSource Inc., San Diego, CA, GNAI1 (BioSS Inc, Woburn, MA) and Ubiquitin (Millipore, Temecula, CA). TEC, PAK6, GNA15, GNG2, GNAI1, STAT2, and SRC were detected using the second antibody donkey anti rabbit Alexa fluora 488. Ubiquitin was detected with donkey anti-mouse Alexa fluora 594 (Jackson Labs, West Grove, PA). All slides were stained with the nuclear stain DAPI (Molecular Probes, Eugene, OR). The fluorescence intensity of stained proteins of interest was measured quantitatively using a 40× objective and a standard exposure time of 800ms using a Nikon 400 fluorescent microscope with three filters (FITC-green, Texas Red, and Tri-Color), and the Nikon morphometric measurement system (Liu et al., 2015 (link)). PIK3CB staining protocol was followed as in previous a publication (Afifiyan et al., 2017 (link)).
Afifiyan N., Tillman B., French B.A., Sweeny O., Masouminia M., Samadzadeh S, & French S.W. (2017). The Role of Tec Kinase Signaling Pathways in the Development of Mallory Denk Bodies in balloon cells in Alcoholic Hepatitis. Experimental and molecular pathology, 103(2), 191-199.
+ Open protocol
+ Expand
2

Immunofluorescent Staining of UFM1 and FAT10

Check if the same lab product or an alternative is used in the 5 most similar protocols
Formalin fixed, paraffin embedded tissue slides were double stained for UFM 1 (Abcam Inc., Cambridge MA) and Ubiquitin (Millipore, Temecula, CA). A second set of slides were stained for FAT10 (Enzo Life Sciences, Farmingdale, NY) and Ubiquitin (Millipore, Temecula, CA). UFM 1 and FAT10 were detected using the second antibody donkey anti rabbit Alexa Fluor 488 (Jackson Immuno Research Laboratories Inc. West Grove, PA). Ubiquitin was detected using the second antibody donkey anti mouse Alexa Fluor 594 (Jackson Labs. West Grove, PA). All slides were stained with the nuclear stain DAPI (Molecular Probes, Eugene, OR). The slides were examined with a Nikon 400 fluorescent microscope.
Liu H., Li J., Tillman B., French B, & French S. (2014). Ufmylation and FATylation Pathways are Down Regulated in Human Alcoholic and Non Alcoholic Steatohepatitis, and Mice Fed DDC, where Mallory-Denk Bodies (MDBs) Form. Experimental and molecular pathology, 97(1), 81-88.
+ Open protocol
+ Expand
3

Quantitative Immunofluorescence Analysis of Complement Proteins in FFPE Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
FFPE tissue slides were triple stained for various complements (Rabbit anti-C1q, Bioss, Woburn, MA; Rabbit anti-C3 and Rabbit anti-C5, Abcam, Cambridge, MA; Rabbit anti-C5aR, Novus Biologicals, San Diego, CA) and ubiquitin (Millipore, Temecula CA).
The complements were detected using the second antibody donkey anti-rabbit Alexa Fluor 488 (Jackson Lab, West Grove, PA). ubiquitin was detected using the second antibody donkey anti-mouse Alexa Fluor 594 (Jackson Lab, West Grove, PA). All slides were stained with the nuclear stain DAPI (Molecular Probes, Eugene, OR). The slides were examined with a Nikon 400 fluorescent microscope. The fluorescence intensity of staining of the protein of interest was measured quantitatively using 40× objectives and a standard exposure time of 800 ms using a Nikon 400 fluorescent microscope with three filters (FITC-green, Texas Red, and Tri-Color), and the Nikon morphometric system. The results were displayed as a graph attached to the immunofluorescent photography using a screen snip.
Shen H., French B.A., Liu H., Tillman B.C, & French S.W. (2014). Increased activity of the complement system in the liver of patients with alcoholic hepatitis. Experimental and molecular pathology, 97(3), 338-344.
+ Open protocol
+ Expand
4

Quantitative Immunofluorescence Analysis of Protein Localization in Liver Biopsy Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
FFPE liver biopsy tissue slides were double stained for ubiquitin (Millipore, Temecula, CA) and the second protein as listed in Table 1. ubiquitin was detected using the second antibody donkey anti-mouse Alexa Fluor 594 (Jackson Labs, West Grove, PA), while the other protein was detected using the second antibody donkey anti-rabbit Alexa Fluor 488 or donkey anti-mouse Alexa Fluor 488 (Jackson Labs, West Grove, PA). All slides were stained with the nuclear stain DAPI (Molecular Probes, Eugene, OR). The fluorescence intensity of staining of the protein of interest was measured quantitatively using 40x objective magnification and a standard exposure time of 800 ms using a Nikon 400 fluorescent microscope with three filters (FITC-green, Texas-red, and tricolor), and the Nikon morphometric system. The results were displayed as a graph attached to the immunofluorescent photography using a screen snip. The staining was compared among MDB forming AH hepatocytes, neighboring non-MDB forming AH hepatocytes, and control normal hepatocytes.
Peng Y., French B.A., Tillman B., Morgan T, & French S.W. (2014). The inflammasome in alcoholic hepatitis: its relationship with Mallory-Denk body formation. Experimental and molecular pathology, 97(2), 305-313.
+ Open protocol
+ Expand
5

Ubiquitin and Chaperone Protein Localization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissue slides were then prepared from the FFPE blocks. The slides were double stained for ubiquitin (Millipore, Temecula, CA) and either Mca1, Hsp104, Hsp40, Ydj1, Ssa1, VCP/p97, or p62. ubiquitin was detected using the red fluorescent(Texas Red) antibody, donkey anti-mouse Alexa Fluor 594 (Jackson Labs, West Grove, PA), while Mca1, Hsp104, Hsp40, Ydj1, Ssa1, VCP/p97, and p62 were detected using the green fluorescent(FITC) antibody, donkey anti-rabbit Alexa Fluor 488 or donkey anti-mouse Alexa Fluor 488 (Jackson Labs, West Grove, PA). The nuclei were stained with DAPI blue. The double stain was detected using a tricolor filter. All biopsies were stained at one time to allow accurate comparisons between groups.
MENDOZA A.S., Dorce J., Peng Y., French B.A., Tillman B., Li J, & French S.W. (2014). Levels of Metacaspase1 and Chaperones Related to Protein Quality Control in Alcoholic and Nonalcoholic Steatohepatitis. Experimental and molecular pathology, 98(1), 65-72.
+ Open protocol
+ Expand
6

Quantitative Immunofluorescence Analysis of BRCA, Cell Cycle, and Apoptosis Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
FFPE tissue slides were double stained for BRCA1, BRCA2, p15, Bax and Tec (Abcam Inc., Cambridge MA) with Ubiquitin (Millipore, Temecula, CA). A second set of slides were stained for BRCA1, BRCA2, p15, Bax and Tec (Enzo Life Sciences, Farmingdale, NY) with Ubiquitin (Millipore, Temecula, CA). BRCA1, BRCA2, p15, Bax and Tec were detected using donkey anti rabbit Alexa Fluor 488 (Jackson Immuno Research Laboratories Inc. West Grove, PA). Ubiquitin was detected using donkey anti mouse Alexa Fluor 594 (Jackson Labs. West Grove, PA). All slides were stained with the nuclear stain DAPI (Molecular Probes, Eugene, OR). The fluorescence intensity of stained protein of interest was measured quantitatively using a 40× objective and a standard exposure time of 800ms using a Nikon 400 fluorescent microscope with three filters (FITC-green, Texas Red, and Tri-Color), and the Nikon morphometric system. The results were displayed as a graph attached to the immunofluorescent photography using a screen snip.
Liu H., Gong M., French B.A., Liao G., Li J., Tillman B, & French S.W. (2015). Aberrant modulation of the BRCA1 and G1/S cell cycle pathways in alcoholic hepatitis patients with Mallory Denk Bodies revealed by RNA sequencing. Oncotarget, 6(40), 42491-42503.
+ Open protocol
+ Expand
7

Double Immunofluorescence Staining of Tissue Slides

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Formalin fixed, paraffin embedded tissue slides were double stained for SYK (Abcam Inc., Cambridge MA) and Ubiquitin (Millipore, Temecula, CA). A second set of slides were stained for AKT1 (Invitrogen, Camarillo, CA) and Ubiquitin. SYK and AKT1 were detected using the second antibody donkey anti rabbit Alexa fluora 488 and anti-mouse Alexa fluora 594 (Jackson Labs, West Grove, PA) respectively. Ubiquitin was detected using the second antibody donkey anti mouse Alexa Fluor 594 (Jackson Labs. West Grove, PA). All slides were stained with the nuclear stain DAPI (Molecular Probes, Eugene, OR). The fluorescence intensity of stained protein of interest was measured quantitatively using a 40× objective and a standard exposure time of 800ms using a Nikon 400 fluorescent microscope with three filters (FITC-green, Texas Red, and Tri-Color), and the Nikon morphometric system (Liu et al., 2015 (link)).
Afifiyan N., Tillman B., French B., Masouminia M., Samadzadeh S, & French S. (2017). Over expression of Proteins that Alter the Intracellular Signaling Pathways in the Cytoplasm of the Liver Cells Forming Mallory-Denk Bodies. Experimental and molecular pathology, 102(1), 106-114.
+ Open protocol
+ Expand
8

Quantitative Analysis of CXCR2 and IL-8 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
FFPE tissue slides were double stained for CXCR2 and IL-8 (Abcam Inc., Cambridge MA) with Ubiquitin (Millipore, Temecula, CA). A second set of slides was stained for CXCR2 and IL-8 (Enzo Life Sciences, Farmingdale, NY) with Ubiquitin (Millipore, Temecula, CA). CXCR2 and IL-8 were detected using donkey anti rabbit Alexa Fluor 488 (Jackson Immuno Research Laboratories Inc. West Grove, PA). Ubiquitin was detected using donkey anti mouse Alexa Fluor 594 (Jackson Labs. West Grove, PA). All slides were stained with the nuclear stain DAPI (Molecular Probes, Eugene, OR). The fluorescence intensity of stained protein of interest was measured quantitatively using a 40× objective and a standard exposure time of 800ms using a Nikon 400 fluorescent microscope with three filters (FITC-green, Texas Red, and Tri-Color), and the Nikon morphometric system.
Liu H., French B.A., Nelson T.J., Li J., Tillman B, & French S.W. (2015). IL-8 signaling is up regulated in alcoholic hepatitis and DDC fed mice with Mallory Denk Bodies (MDBs) present. Experimental and molecular pathology, 99(2), 320-325.
+ Open protocol
+ Expand
9

Dual Immunofluorescence Staining of CXCR4 and CXCR7

Check if the same lab product or an alternative is used in the 5 most similar protocols
Formalin fixed, paraffin embedded tissue slides were double stained for CXCR4 (Abcam Inc., Cambridge MA) and Ubiquitin (Millipore, Temecula CA). A second set of slides was double stained for CXCR7 (Millipore, Temecula CA) and Ubiquitin (Millipore, Temecula, CA. CXCR4 and CXCR7 were detected using the second antibody donkey anti rabbit Alexa Fluor 488 (Jackson Immuno Research Laboratories Inc. West Grove, PA). Ubiquitin was detected using the second antibody donkey anti mouse Alexa Fluor 594 (Jackson Labs. West Grove, PA). All slides were stained with the nuclear stain DAPI (Molecular Probes, Eugene, OR). The slides were examined with a Nikon 400 fluorescent microscope.
Liu H., Li J., Tillman B., Morgan T., French B.A, & French S.W. (2014). TLR3/4 Signaling is Mediated via the NFκB-CXCR4/7 Pathway in Human Alcoholic Hepatitis and Non Alcoholic Steatohepatitis Which Formed Mallory-Denk Bodies. Experimental and molecular pathology, 97(2), 234-240.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!