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Trans ned19

Manufactured by Bio-Techne
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Sourced in United Kingdom, Germany

Trans-Ned19 is a laboratory equipment product. It is designed to perform a specific function in a laboratory setting.

Automatically generated - may contain errors

Lab products found in correlation

Fura 2 am, by Thermo Fisher Scientific (2 mentions) Rottlerin, by Bio-Techne (1 mentions) Go6976, by Bio-Techne (1 mentions) Skf96365, by Bio-Techne (1 mentions) Xestospongin c, by Santa Cruz Biotechnology (1 mentions) 8 br adpr, by Biolog (1 mentions) Ara 2 f nad, by Biolog (1 mentions) Calciumgreen am, by Thermo Fisher Scientific (1 mentions) Wga alexa fluor 594, by Thermo Fisher Scientific (1 mentions) Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions) Hrp conjugated goat anti rabbit igg antibody, by Bio-Rad (1 mentions) Er tracker red, by Thermo Fisher Scientific (1 mentions) Mitotracker deep red, by Thermo Fisher Scientific (1 mentions) U73343, by Bio-Techne (1 mentions) U73122, by Bio-Techne (1 mentions)

3 protocols using trans ned19

1

Inhibitors of Sarcoplasmic Reticulum Calcium Release

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Xestospongin C was obtained from Santa Cruz Biotechnology. Trans-Ned19, GF10923X, Go6976, rottlerin, and SKF96365 were obtained from Tocris Bioscience. 8-Br-ADPR and ara-2’-F-NAD were obtained from Biolog Life Science Institute. All other reagents were obtained from Sigma-Aldrich.
Rah S.Y., Joe Y., Park J., Ryter S.W., Park C., Chung H.T, & Kim U.H. (2023). CD38/ADP-ribose/TRPM2-mediated nuclear Ca2+ signaling is essential for hepatic gluconeogenesis in fasting and diabetes. Experimental & Molecular Medicine, 55(7), 1492-1505.
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2

Intracellular Calcium Signaling Assay

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cis-NED 19, trans-NED 19, U73122, and U73343 were acquired from Tocris (Bristol, UK); Fura2/AM, CalciumGreen/AM, and LysoTracker Red DND-99 from ThermoFischer Scientific (Waltham, MA, USA); Mitotracker Deep Red (M22426, Invitrogen), ER-tracker Red (E34250, Invitrogen), WGA Alexa Fluor 594 (Thermo Fisher, W11262), and antibodies anti-TPC1 (#ACC-071) and anti-TPC2 (#ACC-072) from Alomone Labs (Jerusalem, Israel); HRP-conjugated goat anti-rabbit IgG antibodies from BioRad (#170-6515); and the agonists of the receptors from SigmaAldrich (St. Louis, MO, USA).
Trufanov S.K., Rybakova E.Y., Avdonin P.P., Tsitrina A.A., Zharkikh I.L., Goncharov N.V., Jenkins R.O, & Avdonin P.V. (2019). The Role of Two-Pore Channels in Norepinephrine-Induced [Ca2+]i Rise in Rat Aortic Smooth Muscle Cells and Aorta Contraction. Cells, 8(10), 1144.
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3

Fura2-AM Cytosolic Calcium Assay

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Fura2-AM was obtained from Life Technologies (Carlsbad, USA). The dye was dissolved in DMSO, divided into aliquots, and stored at −20 °C until required for use. Anti-mouse CD3 mAb (145-2C11), anti-mouse CD28 mAb (37.51), anti-mouse IFN-γ (XMG1.2), and anti-mouse IL-4 (11B11) were produced in house. Trans-Ned 19 was obtained from Tocris (Bremen, Germany) and BZ194 from Dept. of Organic Chemistry (University of Hamburg, Germany). BZ194 was synthesized according to the procedure reported in [17 (link)] All other chemicals were from Aldrich-Sigma-Merck (Darmstadt, Germany).
Nawrocki M., Lory N., Bedke T., Stumme F., Diercks B.P., Guse A.H., Meier C., Gagliani N., Mittrücker H.W, & Huber S. (2021). Trans-Ned 19-Mediated Antagonism of Nicotinic Acid Adenine Nucleotide—Mediated Calcium Signaling Regulates Th17 Cell Plasticity in Mice. Cells, 10(11), 3039.
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