MKL-1, MKL-2, MS-1, MCC301, MCC336, and MCC350 were initially Accutase-treated (STEMCELL). Cells were plated with 0.2% DMSO, tazemetostat, or EED226 (Cayman Chemical; Ann Arbor, MI). Inducible lines were treated with 1.5 μg/mL doxycycline. Every 3 days each line was split according to its vehicle condition’s needs with inhibitor/doxycycline refreshment. Cells were lysed with CellTiter-Glo (Promega; Madison, WI).
Eed226
Manufactured by Cayman Chemical
EED226 is a laboratory instrument used for the detection and measurement of electrochemical events. It is designed to provide accurate and reliable data for various applications in the field of electrochemistry.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using eed226
1
Cellular Assays with Epigenetic Modulators
2
Naïve H9 hESC Maintenance Optimization
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Naïve H9 hESCs were maintained in NaïveCult expansion medium supplemented with 10 µM EED226 (Cayman Chemical, CAYM22031-10) or 2.5 µM UNC1999 (Sigma, SML0778). The latter was also applied to naïve H9 hESCs maintained in PXGL medium. Treatments lasted for 7 days, and the medium was changed daily. All cells were passaged on d3-4 counting from the start of treatment. On day 7, cells were fixed using 4% paraformaldehyde (FF-Chemicals, FFCHFF22023000) for 10 min. Samples were left in DPBS−/− (Gibco, 14190144) at 4 °C until proceeding with the immunofluorescence protocol.
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