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3 protocols using cefazolin

1

Antibiotic Susceptibility Testing Protocol

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Antibiotic agents (oxacillin, cefazolin, cefuroxime, clindamycin, erythromycin, levofloxacin, moxifloxacin, tetracycline, tigecycline, amikacin, fosfomycin, trimethoprim-sulfamethoxazole, linezolid, vancomycin, daptomycin, rifampin) were purchased from Dalian Meilun Biotech (Dalian, China). Glucose-6-phosphate was obtained from Sigma-Aldrich Co. (St Louis, MO, USA). tigecycline and daptomycin susceptibility test were done via broth dilution method according to CLSI recommendations. The MICs of the other 15 antibiotics were determined using the twofold serial agar dilution method.9
E. faecalis ATCC 29212 were used as quality-control strains.
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2

Antimicrobial Agents Evaluation Protocol

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The antimicrobials utilized in this work were cefazolin, gentamicin, erythromycin, tigecycline, rifampicin, daptomycin (purchased from Dalian Meilun Biotech, Dalin, China), ciprofloxacin, clindamycin, vancomycin (from the National Institutes for Food and Drug Control, Beijing, China), linezolid (Selleck Chemicals, Houston, TX, United States), and trimethoprim–sulfamethoxazole (Sigma–Aldrich, St Louis, MO, United States).
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3

Isolation and Characterization of Flavaspidic Acid BB

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Flavaspidic acid BB (BB, purity > 95%) was isolated from D. fragrans using silica gel column chromatography, Sephadex LH-20 column chromatography, and pre-HPLC with some modifications in the preliminary experiments [26 (link)–28 (link)]. Erythromycin (E, N0825A, 85%), mupirocin (MUP, 17,090,377, 95%), fusidic acid (FD, Z19J6Q1, 98%), cefazolin (190,501, 98%) and dimethyl sulfoxide (DMSO) were purchased from Dalian Meilun Biotechnology Inc. (Dalian, China), Sino-US Tianjin SK Pharmaceutical Inc. (Tianjin, China), Shanghai Yuanye Biotechnology Inc. (Shanghai, China), Guangdong South China Pharmaceutical Group Inc. (Guangzhou, China), and Sigma-Aldrich (Saint Louis, USA), respectively. Flavaspidic acid BB was dissolved in DMSO at a concentration of 51.2 mg/mL. E, MUP, and FD were dissolved in DMSO as a drug reserve solution (512 mg/ml) and stored at -20 °C.
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