Gapdh ga1r

Manufactured by Thermo Fisher Scientific

GAPDH (GA1R) is a lab equipment product from Thermo Fisher Scientific. It is a glyceraldehyde-3-phosphate dehydrogenase, an enzyme involved in the glycolytic pathway. The core function of GAPDH (GA1R) is to catalyze the reversible oxidation of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate.

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Lab products found in correlation

Anti tubulin, by Merck Group (1 mentions) Cd103 2e7, by BioLegend (1 mentions) Tcrβ h57 597, by BioLegend (1 mentions) β actin ba3r, by Thermo Fisher Scientific (1 mentions) Aif1 epr16588 antibody, by Abcam (1 mentions) P38 d13e1, by Cell Signaling Technology (1 mentions) Biotin, by Fortis Life Sciences (1 mentions) Actin hrp, by Merck Group (1 mentions) Ubiquitin p4d1, by Cell Signaling Technology (1 mentions) Flag m2, by Merck Group (1 mentions) Tfam d5c8, by Cell Signaling Technology (1 mentions) Cyclin b1 gns1, by SCBT (1 mentions) Map lc3β g 9, by SCBT (1 mentions) Opa1 612606, by BD (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Hrp conjugated goat anti rabbit or goat anti mouse igg, by Jackson ImmunoResearch (1 mentions) Goat anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions)

5 protocols using gapdh ga1r

Cell Cycle Regulation Mechanisms

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Palbociclib (PD-0332991, IBRANCE®), Ribociclib (LEE-011, Kiskali®), Mitotane and PNU-74654 were purchased from CliniSciences (A8316, A8641, sc-205754 and sc-258020, respectively). Palbociclib and Ribociclib 1mM stock solutions were prepared in 100% ethanol or DMSO respectively. PNU-74654 stock solution (31.2 mM) was prepared in DMSO. Anti-CDK6 (D4S8S), CDK4 (D9G3E), Phospho-Rb (9308), GSK3-β (D5C5Z), Phospho-GSK3-β (D85E12), non-phospho-β-Catenin (D13A1) were purchased from Cell Signaling Technology. The anti-β-Catenin (MA1-301) and GAPDH (GA1R) were purchased from Thermo Fischer Scientific. The anti-ɑ-Tubulin (T9026) was purchased from Sigma.

Hadjadj D., Kim S.J., Denecker T., Driss L.B., Cadoret J.C., Maric C., Baldacci G, & Fauchereau F. (2017). A hypothesis-driven approach identifies CDK4 and CDK6 inhibitors as candidate drugs for treatments of adrenocortical carcinomas. Aging (Albany NY), 9(12), 2695-2716.

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Phenotypic Analysis of Hematopoietic Cells

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AIF1 (EPR16588) antibody used was purchased from Abcam (Cambridge MA). CD117 (2B8), Lineage cocktail (CD3/Gr-1/CD11b/CD45R/TER-119), SCA1 (D7), CD34 (SA376A4), CD11c (N418), MHC class II (M5/114.15.2), CD8α (53–6.7), CD11b (M1/70), F4/80 (BM8), CD45R (RA3-6B2), TCRβ (H57-597), Protein Kinase C (PKC; PKC0103), CD4 (RM4-5), CD3 (17A2), IgD (11-26c.2a), CD19 (6D5), CD24 (M1/69), PDCA1 (927), CD172α (P84), IL-7Rα (A7R34), CD135 (A2F10), and CD103 (2E7) purchased from BioLegend. RelB (C1E4), NFκB p100/P52 (D7A9K), phospho-IκBα (S32), IκBα (L35A5), phosho-p38 (M139), and p38 (D13E1) purchased from Cell Signaling Technology (Danvers MA). Phospho-ERK (M206) purchased from ECM biosciences (Versailles KY) and IRF4 (343), IRF8 (V3GYWCH), NFκB p105/50 (catalog #14-6732-81), GAPDH (GA1R), and β-actin (BA3R) from Thermo Fisher. Zbtb46 (U4-1374) purchased from BD Biosciences (San Jose CA). Live/Dead fixable dead cell stain (cat# L23101) purchased from Thermo Fisher Scientific.

Elizondo D.M., Brandy N.Z., da Silva R.L., Haddock N.L., Kacsinta A.D., de Moura T.R, & Lipscomb M.W. (2019). Allograft Inflammatory Factor-1 Governs Hematopoietic Stem Cell Differentiation Into cDC1 and Monocyte-Derived Dendritic Cells Through IRF8 and RelB in vitro. Frontiers in Immunology, 10, 173.

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Immunoblotting of Synaptic Proteins

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Samples were electrophoresed on 4–20% Tris-Glycine gradient gels and transferred to nitrocellulose membrane for immunoblotting using the following antibodies:
From the Developmental Studies Hybridoma Bank: Brp (nc82) 1:50; Synapsin-1 (3C11) 1:500; Syntaxin (8C3) 1:500; Drpr (1:1 mix of 5D14:8A1) 1:400; Repo (8D12) 1:200. Other antibodies used were biotin (Bethyl Laboratories) 1:1000; FLAG (M2) (Sigma) 1:500; TH (Immunostar) 1:1000; Actin-HRP (Sigma) 1:1000; Ubiquitin (P4D1) (Cell Signaling Technology) 1:1000; GAPDH (GA1R) (ThermoFisher Scientific) 1:10,000.

Villalobos-Cantor S., Barrett R.M., Condon A.F., Arreola-Bustos A., Rodriguez K.M., Cohen M.S, & Martin I. (2023). Rapid cell type-specific nascent proteome labeling in Drosophila. eLife, 12, e83545.

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Whole Cell Extract Preparation and Immunoblotting

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To prepare whole cell extracts from exponentially growing cultures, cells were lysed in RIPA lysis buffer [50 mM Tris, pH 8.0; 150 mM NaCl, 5 mM EDTA (pH 8.0), 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor cocktail (Pierce)]. Lysates were cleared by centrifugation at 12,000 rpm for 15 min and the supernatant was used for Western Blotting. Antibodies for immunoblotting were: TFAM [D5C8] (Cell Signaling), Drp1 [6Z-82] (SCBT), phospho-Drp1-Ser616 [D9A1] (Cell Signaling), cyclin B1 [GNS1] (SCBT), cyclin A [BF683] (SCBT), Ran [610340] (BD Biosciences), MAP LC3β [G-9] (SCBT), Mfn1 [D-10] (SCBT), GAPDH [GA1R] (Invitrogen), Opa1 [612606] (BD Biosciences), Nrf1 [147.1] (SCBT) and Nrf2 [A-10] (SCBT). Image quantification was performed in ImageJ (version 1.53a; freely available at http://imageJ.nih.gov/ij)^{90 (link)}. Full-size membranes of Western blots from which panels in Figs. 2–5 were prepared are shown in Supplemental Fig. S5–S8.

Subramanian V., Rodemoyer B., Shastri V., Rasmussen L.J., Desler C, & Schmidt K.H. (2021). Bloom syndrome DNA helicase deficiency is associated with oxidative stress and mitochondrial network changes. Scientific Reports, 11, 2157.

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Antibody Panel for Golgi Protein Analysis

Cited 1 time

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We used the following primary antibodies: mouse monoclonal antibody against mAID (clone 1E4, catalog #M214-3; MBL), FLAG-tag (M2, catalog #F3165; Sigma), GAPDH (GA1R, catalog #MA5-15738; Invitrogen), Golgin-45 (E-3, catalog #sc-515193; Santa Cruz Biotechnology), Golgin-97 (CDF4, catalog #A-21270; Invitrogen), GM130 (35/GM130, catalog #610822; BD Transduction Labs), GRASP55 (1C9A3, catalog #66627–1-lg; Proteintech), GRASP65 (D-12, catalog #sc-374423; Santa Cruz Biotechnology), p115 (4H1; Waters et al., 1992 (link)), and α-tubulin (DM1A, catalog #14–4502-80; eBioscience) and rabbit polyclonal antibodies against Golgin-84 (Beard et al., 2005 (link)), GM130 (Wei et al., 2015 (link)), GRASP55 (catalog #PTG10598-1-AP; Proteintech), GRASP65 (UT465; Wei et al., 2015 (link)), Myc-tag (A-14, catalog #sc-789; Santa Cruz Biotechnology), and p115 (catalog #130509–1-AP; Proteintech). Secondary antibodies were as follows: Alexa Fluor 488–, Alexa Fluor 555–, Alexa Fluor 594–, or Alexa Fluor 647–conjugated highly cross-absorbed goat anti-mouse IgG (H+L) or goat anti-rabbit IgG (H+L; Invitrogen), HRP-conjugated goat anti-rabbit, or goat anti-mouse IgG (Jackson ImmunoResearch).

Zhang Y, & Seemann J. (2020). Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure. The Journal of Cell Biology, 220(1), e202007052.

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