Sk n be 2 c

The human SH-SY5Y, SK-N-AS, IMR-32, SK-N-BE2c and HEK293T/17 cell lines were obtained from the American Type Culture Collection (respectively ATCC #CRL-2266, #CRL-2137, #CCL-127, #CRL-2268 and #CRL-11268). SH-SY5Y, SK-N-AS and HEK293T/17 cell lines were grown in Dulbecco’s modified Eagle’s medium (DMEM, Sigma); IMR32 cell line was grown in minimum essential medium eagle (MEM, Sigma) and SK-N-BE2c cell line was grown in DMEM/F12 medium (Sigma). The medium were supplemented with 10% heat-inactivated FBS (Sigma), 1mM L-glutamine, penicillin (100 U/ml) and streptomycin (100 μg/ml) (Invitrogen). The cells were cultured at 37°C, 5% CO2 in a humidified atmosphere. The cumulative culture length of the cells was fewer than 6 months after resuscitation. Early passage cells were used for all experiments and they were not re-authenticated.