Log In Sign up
The largest database of trusted experimental protocols

Takinib

Manufactured by MedChemExpress
Visit Supplier

Takinib is a laboratory equipment product manufactured by MedChemExpress. It is a chemical compound that functions as a selective inhibitor of a specific protein target. The core function of Takinib is to modulate the activity of this target protein in in-vitro and in-vivo research settings.

Automatically generated - may contain errors

Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Ha 3f10, by Roche (1 mentions) Gfp a6455, by Thermo Fisher Scientific (1 mentions) Phospho p38 mapk 9211, by Cell Signaling Technology (1 mentions) Phospho jnk 9251, by Cell Signaling Technology (1 mentions) Alexa fluor 594 and alexa fluor 488 conjugated secondary antibodies, by Thermo Fisher Scientific (1 mentions) Cl075, by InvivoGen (1 mentions) Polydt, by InvivoGen (1 mentions) 5z 7 oxozeaenol, by Bio-Techne (1 mentions) Jnk 9252, by Cell Signaling Technology (1 mentions) High molecular weight poly 1 c, by InvivoGen (1 mentions) Erk 4695, by Cell Signaling Technology (1 mentions) Sb203580, by InvivoGen (1 mentions) Gapdh sc 25778, by Santa Cruz Biotechnology (1 mentions) Hrp conjugated secondary antibody, by GE Healthcare (1 mentions) Smi 312r, by Fortrea (1 mentions) Neuronal nuclei (neun), by Merck Group (1 mentions) Monensin, by Bio-Techne (1 mentions) Propidium iodide, by ImmunoChemistry Technologies (1 mentions) Filipin 3, by Merck Group (1 mentions)

3 protocols using takinib

1

Immunohistochemical Analysis of Neuronal Signaling

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The antibodies and reagents used in this study were as follows: GFP (A6455; rabbit; Invitrogen; Chen et al., 2011 (link)); MAP2 (AB5622; rabbit; EMD Millipore; Chen et al., 2011 (link)); MAP2 (M4403; mouse; Sigma-Aldrich; Chen et al., 2011 (link)); SMI-312R (SMI-312R; mouse; Covance; Liu et al., 2013 (link)); HA (3F10; rat; Roche; Chen et al., 2017 (link)); phospho-P38 MAPK (9211; rabbit; Cell Signaling Technology); rabbit polyclonal P38 MAPK antibody (9212; rabbit; Cell Signaling Technology); phospho-ERK (4376; rabbit; Cell Signaling Technology); ERK (4695; rabbit; Cell Signaling Technology); phospho-JNK (9251; rabbit; Cell Signaling Technology); JNK (9252; rabbit; Cell Signaling Technology); phospho-TAK1 (9339; rabbit; Cell Signaling Technology); GAPDH (sc-25778; rabbit; Santa Cruz Biotechnology, Inc.; Chen et al., 2011 (link)); NeuN (MAB377; mouse; EMD Millipore; Wang et al., 2015 (link)); HRP-conjugated secondary antibodies (GE Healthcare); and Alexa Fluor 488– and Alexa Fluor 594–conjugated secondary antibodies (Invitrogen). Antibodies with validation profiles in Antibodypedia or 1DegreeBio are underlined. CL075, poly dT, poly(I:C) high molecular weight, and SB203580 were all purchased from InvivoGen. Takinib and NG 25 were purchased from Medchem Express, and (5Z)-7-oxozeaenol was purchased from Tocris.
Hung Y.F., Chen C.Y., Shih Y.C., Liu H.Y., Huang C.M, & Hsueh Y.P. (2018). Endosomal TLR3, TLR7, and TLR8 control neuronal morphology through different transcriptional programs. The Journal of Cell Biology, 217(8), 2727-2742.
+ Open protocol
+ Expand
2

Inflammasome Activation Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
All reagents and solvents
were purchased from
commercial suppliers (Sigma-Aldrich, Alfa Aesar, Fluka, TCI America).
Ultrapure LPS, anti-IL-1β antibody, isoliquiritigenin, and MCC950
were purchased from Invivogen. All cell media, Hoechst 33342, DQ Green
BSA, and Alexa Flour 488-NHS were bought from Thermo Fischer Scientific.
Lysoview 633 was obtained from Biotium. APG-4 was purchased from TEFLabs,
monensin from Tocris, and filipin-III from Sigma-Aldrich. Propidium
iodide, FAM-YVAD-FMK kit (FAM FLICA Caspase-1), and cathepsin B MM-(RR)2 kit (Magic Red cathepsin B) were bought from Immunochemistry
Technologies. Ca-074-Me was obtained from Calbiochem. E 64d and (5Z)-7-Oxozeaenol
were from Tocris. Takinib was from Medchemexpress. Rabbit polyclonal
anti-TMS1 and donkey antirabbit IgG Alexa Fluor 647 antibodies were
purchased from Abcam. All peptide synthesis reagents were purchased
from Novabiochem. Fmoc-N-amido-dPEG6-acid linker was purchased
from Quanta Biodesign, and azidohexanoic acid was purchased from Click
Chemistry Tools.
Manna S., Howitz W.J., Oldenhuis N.J., Eldredge A.C., Shen J., Nihesh F.N., Lodoen M.B., Guan Z, & Esser-Kahn A.P. (2018). Immunomodulation of the NLRP3 Inflammasome through Structure-Based Activator Design and Functional Regulation via Lysosomal Rupture. ACS Central Science, 4(8), 982-995.
+ Open protocol
+ Expand
3

Reagents for Cellular Stress Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following reagents were used in our study: collagen (Roche, Indianapolis, IN; 11179179001), G418 (Invitrogen, Carlsbad, CA), recombinant human TNFα (PeproTech, Rocky Hill, NJ; 300-01A), anisomycin (Fermentek, Jerusalem, Israel; AN), arsenite (Fluka, Ronkonkoma, NY; 71287). Sorbitol (S1876), sodium palmitate (P9767), puromycin (P7255), 5Z-7-Oxozeaenol (O9890), and cycloheximide (C7698) were all purchased from Sigma-Aldrich (St. Louis, MO). URMC-099 was purchased from AdooQ BioScience LLC (Irvine, CA; A14219). Takinib was purchased from MedChemExpress (Monmouth Junction, NJ; HY-103490). CEP-1347 was purchased from Tocris Bioscience (Bristol, UK; 4924).
Prinz E., Aviram S, & Aronheim A. (2018). WDR62 mediates TNFα-dependent JNK activation via TRAF2-MLK3 axis. Molecular Biology of the Cell, 29(20), 2470-2480.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!