The purity analysis of ONP was performed on Waters pump 1525 and PDA 2998 series HPLC systems with reversed-phase C18 (4.6 × 150 mm, 5 µm) chromatographic columns at room temperature. ONP was dissolved in chromatographic methanol, and the injection volume was 10 µL. The mobile phases are methanol and water, and the flow rate is 1.0 mL/min. The maximum absorbance in the range of 210–400 nm is used as the detection wavelength.
Pda 2998 series
Manufactured by Waters Corporation
Sourced in United States
The PDA 2998 series is a photodiode array (PDA) detector designed for high-performance liquid chromatography (HPLC) and other analytical applications. It provides multi-wavelength detection capabilities, allowing for the simultaneous monitoring of multiple analytes in a sample. The PDA 2998 series offers a wide wavelength range and high sensitivity to support a variety of analytical requirements.
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Lab products found in correlation
2 protocols using pda 2998 series
1
HPLC Purity Analysis of ONP
2
Colchicine Quantification in Medicinal Plants
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The analysis of the colchicine was done by using HPLC system called WATERS (Milford, USA) having Waters PDA 2998 series Eur J Biol 2020; 79(2): 67-74 Maqsood et al. Colchicine Quantification in Salt Treated Colchicum luteum photodiode array detector set at wavelength range 190-800 nm. The column from the Waters Spherisorb® C18 bonded with 5 µm (4.6 x 250 mm) accompanied with EMPOWER-2 software was utilized for collection and processing of chromatographic data. Ultrasonic cleaner (Steryl medi-equip systems) and water purification system ELIX 03 (MILLIPORE, USA) was also used.
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