Pe conjugated cd40 monoclonal antibody

Aminated magnetic MSN was obtained from So-Fe Biomedical Co., Ltd. (Shanghai, China). ICG was purchased from Meryer Chemical Technology Co., Ltd. (Shanghai, China). DiO, DiI, LysoTracker™ Red, and the BCA protein assay kit were purchased from the Beyotime Institute of Biotechnology (Shanghai, China). Primers used for the reactions were purchased from Wuhan Gene Create Biological Engineering Co., Ltd. (Wuhan, China). GM-CSF, IL-4 and 5(6)-CFDA N-succinimidyl ester (CFSE) were purchased from Abcam (Cambridge, UK). FITC-conjugated anti-mouse CD11c antibody was purchased from BioLegend (San Diego, USA). PE-conjugated CD86 monoclonal antibody, PE-conjugated CD80 monoclonal antibody, PE-conjugated CD40 monoclonal antibody, APC-conjugated CCR monoclonal antibody, APC-conjugated MHC-I monoclonal antibody, APC-conjugated MHC-II monoclonal antibody, PE-conjugated CD4 monoclonal antibody, APC-conjugated CD8 monoclonal antibody, and carboxy-H₂DCFDA were purchased from Invitrogen (Carlsbad, USA). FITC-conjugated CD3 monoclonal antibody was purchased from eBioscience (CA, USA). Drug-resistant S. aureus BW15 and BWMR26 and drug-sensitive S. aureus S29213 strains were obtained from Dr. Gao (School of Medicine, Yangzhou University). Unless otherwise stated, all other reagents were purchased from the Nanjing Well Offer Biotechnology Co., Ltd. (Nanjing, China).