In the present study, mouse monoclonal anti-MUC2 (ab11197, 1/500 WB and 1/100 IF, Abcam), rabbit monoclonal anti-TFF3 (ab108599, 1/1500 WB, Abcam), mouse monoclonal anti-SI ([53]; HSI-4/34 or Caco-3/73, 1/100 WB), rabbit monoclonal anti-DPPIV or CD26 [EPR20819] (ab215711, 1/2000 WB, Abcam), rabbit monoclonal anti-YAP/TAZ (D24E4, 1/1500 WB, Cell Signaling Technology, Danvers, MA, USA), rabbit monoclonal anti-YAP (D8H1X, 1/1000 WB, 1/150 IF, Cell Signaling Technology), mouse monoclonal anti-TAZ (M2-616, 1/300 IF, BD Biosciences, NJ, USA) mouse monoclonal anti-CDX2-CD88 (MU392A-UC, 1/700, BioGenex, Freemont, CA, USA), mouse monoclonal anti-HNF1α [F-7] (sc-393925, 1/300 WB, Santa Cruz Biotechnology, Dallas, TX, USA), goat polyclonal anti-HNF4α [C-19] (sc-6556, 1/600 WB, Santa Cruz Biotechnology) and anti-β-actin (MAB1501, 1/20,000, Millipore, Etobicoke, ON, Canada) were used as primary antibodies. In addition, AlexaFluor 488 or 594 goat anti-mouse (A11017, A11072, 1/400; Thermo Fisher Scientific, Ottawa, ON, Canada) and goat anti-rabbit (A11070, A11072, 1/400), ECL HRP-linked anti-mouse (NA931V, 1/4000, GE Healthcare, Mississauga, ON, Canada) and anti-rabbit (NA934V, 1/4000) and HRP-linked bovine anti-goat (sc-2350, 1/4000, Santa Cruz Biotechnology) were used as secondary antibodies.
A11070
Manufactured by GE Healthcare
Sourced in Canada
The A11070 is a laboratory centrifuge designed for general-purpose applications. It features a fixed-angle rotor that can accommodate various sample tube sizes. The centrifuge is capable of reaching a maximum speed of 6,000 rpm and a maximum relative centrifugal force (RCF) of 4,200 x g. The unit is equipped with a digital display that shows the speed, time, and other operational parameters.
Automatically generated - may contain errors
Lab products found in correlation
Na931v, by GE Healthcare (2 mentions)
Mab1501, by Merck Group (2 mentions)
A11017, by Thermo Fisher Scientific (2 mentions)
A11072, by GE Healthcare (2 mentions)
M2 616, by BD (1 mentions)
Ab215711, by Abcam (1 mentions)
Ab11197, by Abcam (1 mentions)
Ab108599, by Abcam (1 mentions)
Sc 393925, by Santa Cruz Biotechnology (1 mentions)
Na934v, by Santa Cruz Biotechnology (1 mentions)
Sc 2350, by Santa Cruz Biotechnology (1 mentions)
Sc 6556, by Santa Cruz Biotechnology (1 mentions)
D8h1x, by Cell Signaling Technology (1 mentions)
Mu392a uc, by BioGenex (1 mentions)
A11006, by Thermo Fisher Scientific (1 mentions)
A11007, by Thermo Fisher Scientific (1 mentions)
A 11072, by Thermo Fisher Scientific (1 mentions)
Na934v, by GE Healthcare (1 mentions)
T6793, by Merck Group (1 mentions)
Ab49314, by Abcam (1 mentions)
2 protocols using a11070
1
Immunoblotting and Immunofluorescence Assays
2
Immunoblotting of Tubulin Modifications
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The primary antibodies used in this study were: mouse monoclonal anti-tubulin acetylated 611B-1 (Sigma-Aldrich, T6793, 1/1000), mouse monoclonal anti-polyglutamylation modification of tubulin (AdipoGen, GT335, 1/2000), rat polyclonal anti-CD49f G0H3 (BD Pharmingen, 555734, 1/5000), rabbit polyclonal anti-SMO (Abcam, ab38686, 1/500), rabbit monoclonal anti-GLI1 EPR4523 (Abcam, ab134906, 1/1000 for WB) and (Abcam, ab49314, 1/1000 for IF) and mouse monoclonal anti-β-actin (Millipore, MAB1501, 1/20,000). Preliminary results (unpublished) were obtained using rabbit polyclonal anti-GLI3C 2438B, mouse monoclonal anti-Gli3 N 6F5 and rabbit polyclonal anti-Gli3 N 2676A (Genentech) obtained courtesy of FJ de Sauvage [38] (link). Secondary antibodies were: AlexaFluor 488 or 594 goat anti-mouse (Molecular Probes, A11017, A11072, 1/400), goat anti-rat (Molecular Probes, A11006, A11007, 1/400), goat anti-rabbit (Molecular Probes, A11070, A11072, 1/400), ECL HRP-linked anti-mouse (GE Healthcare, NA931 V, 1/3000) and anti-rabbit (NA934 V, 1/3000).
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