Mouse pituitary corticotroph cells (AtT20, Cat No. CCL-89, ATCC, Manassas, VA, USA) and rat somatotroph cells (GH3, Cat No. CCL-82.1, ATCC) were cultured with low-glucose Dulbecco's modified Eagle medium (DMEM) with 10% fetal bovine serum, 100 U/mL penicillin, and 100 µg/mL streptomycin. These cells (25,000 cells/cm2) were incubated in a humidified incubator at 37℃ with 5% CO2.
Att20
Manufactured by American Type Culture Collection
Sourced in United States
The AtT20 is a piece of laboratory equipment designed for the cultivation and maintenance of cell lines. It provides a controlled environment for the growth and propagation of cells, ensuring optimal conditions for their survival and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Foetal bovine serum (fbs), by Cellmax (1 mentions)
Bexarotene, by Selleck Chemicals (1 mentions)
Dexamethasone (dex), by Selleck Chemicals (1 mentions)
Att 20 d16v f2, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by American Type Culture Collection (1 mentions)
Horse serum, by Thermo Fisher Scientific (1 mentions)
Gt1 1, by Thermo Fisher Scientific (1 mentions)
Att 20, by Thermo Fisher Scientific (1 mentions)
F 12k medium, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Gt1 1, by American Type Culture Collection (1 mentions)
Sb 505124, by MedChemExpress (1 mentions)
Hy 13521, by MedChemExpress (1 mentions)
Rc 4bc, by American Type Culture Collection (1 mentions)
Gdc 0449, by Selleck Chemicals (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
7 protocols using att20
1
Culturing Mouse and Rat Endocrine Cells
2
Cell Culture of Pituitary and Glioblastoma Lines
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The pituitary adenoma cell lines GH3 and AtT-20 (both from ATCC, Manassas, Virginia, US) and the glioblastoma cell line U373 MG (from the original cultures established in Uppsala 1968 [17 (link)]) were cultured as adherent monolayer at 37àC in a 5% CO2 humidified atmosphere. Cells were grown in DMEM/F-12 supplemented with 17% FBS, 100 U/ml penicillin and 100 g/ml streptomycin and DMEM supplemented with 10% FBS, 100 U/ml penicillin and 100 g/ml streptomycin, respectively. Medium was changed every third day and cells were harvested when they reached 80–90% confluence with 0.25% Trypsin-EDTA for further experiments.
3
Culturing Pituitary Corticotroph Cells
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The mouse anterior pituitary corticotroph adenoma cell line AtT‐20/D16v‐F2 was purchased from ATCC, while COS‐7 cell line was purchased from Cell Bank of Type Culture Collection of Chinese Academy of Sciences. AtT‐20 cells and COS‐7 cells were cultured in DMEM (ATCC, 30‐2002) media supplemented with 10% foetal bovine serum (Cellmax, Peking, China) and penicillin‐streptomycin. The cells were incubated at 37°C and 5% CO2 atmosphere. Bexarotene, ATRA, and dexamethasone (DEX) were obtained from Selleck and stored at −80°C. Corticotropin‐releasing factor human (CRH) was obtained from MCE and stored at −80°C.
4
Pituitary Tumor Cell Line Culture and TMZ Treatment
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The mouse pituitary adenoma cell line GT1-1 (ATCC, USA) was cultured in Dulbecco’s modified Eagle medium (DMEM) containing 10% fetal bovine serum (FBS, Gibco, USA) and the mouse pituitary tumor cell line ATt20 (ATCC, USA) were cultured in 12K Medium (F-12K) supplemented with 15% horse serum and 2.5% FBS. Both GT1-1 and ATt20 cells were cultured at 37°C in a 5% CO2 humidified incubator.
Two types of cells were treated with different doses of TMZ (ranged from100 to 500 nM). The IC50 results for ATt20 cells are shown inSupplementary Material 1 .
Two types of cells were treated with different doses of TMZ (ranged from100 to 500 nM). The IC50 results for ATt20 cells are shown in
5
Culturing Mouse Pituitary Cell Lines
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Mouse pituitary adenomas cell lines AtT-20, GT1.1 and pituitary cell MPC were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). MPC cell and GT1.1 cell were cultured in high glucose Dulbecco's modified Eagle's medium (DMEM, Gibco, Life Technologies, Gaithersburg, MD, USA) supplemented with 10% horse serum (Gibco, Life Technologies, Gaithersburg, MD, USA) and 2.5% fetal bovine serum (FBS, Gibco, Life Technologies, Gaithersburg, MD, USA), AtT-20 cell was cultured in F-12K medium (Gibco, Life Technologies, Gaithersburg, MD, USA) supplemented with 10% horse serum (Gibco, Life Technologies, Gaithersburg, MD, USA) and 2.5% FBS (Gibco, Life Technologies, Gaithersburg, MD, USA). All of them were incubated at 37°C in a humidified atmosphere with 5% CO2.
6
Cell Line Cultivation and Inhibitor Treatments
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The GH3, AtT20, MMQ, and RC-4BC cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). GH3, AtT20, and MMQ cell line Twere cultured in Ham's F-12 K medium (L450KJ, BasalMedia) supplemented with 2.5% FBS (S615JY, BasalMedia), 15% horse serum (26,050,088, ThermoFisher), and 1% penicillin/streptomycin (C100C5, NCM biotech. RC-4BC cells were cultured in DMEM (L130KJ, BasalMedia) with 10% FBS, 5 ng/ml recombinant rat EGF (ab290070, Abcam), and 1% penicillin/streptomycin. The following inhibitor and recombinant protein were used: recombinant INHBA (C687, Novoprotein), SB-505124 (HY-13521, MedChemExpress), A 83–01 (HY-10432, MedChemExpress), and recombinant human Follistatin (10685-H08H, Sino Biological).
7
Modulating Corticotropinoma Cell Signaling
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Mouse corticotropinoma cells from the cell line AtT-20 (American Type Culture Collection; passages 28-32) were maintained in 75 cm 2 cell culture flasks in DMEM, 10% fetal bovine serum, 2 mM glutamine and 10 5 U/l penicillin-streptomycin (Invitrogen) at 37 8C and 5% CO 2 . Cells were passaged at 80% confluency and seeded into 12-well plates at 150 000 cells/well as previously described (Reiter et al. 2011) (link). Cells were treated with Hh agonist purmorphamine (Cayman Chemical, Ann Arbor, MI, USA, Cat. No. 10009634), Smoothened antagonist GDC-0449 (Vismodegib; Selleckchem, Houston, TX, USA, Cat. No. S1082), GLI antagonist GANT61 (Sigma-Aldrich, Cat. No. G9048) or an equivalent amount of DMSO as solvent control.
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