Msd 5973

Manufactured by Agilent Technologies

Sourced in United States

The MSD 5973 is a mass selective detector (MSD) designed for use with Agilent gas chromatography (GC) systems. It provides sensitive and selective detection of a wide range of compounds. The MSD 5973 is capable of performing full-scan, selected ion monitoring (SIM), and tandem mass spectrometry (MS/MS) analyses.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Mass Selective Detector MSD 5973 (12 citations) Agilent 5973 MSD (11 citations) 5973 MSD mass spectrometer (10 citations) Agilent 6890 N/5973 N GC-MSD (5 citations) Model 5973 N MSD mass spectrometer (2 citations) MSD 5973 system (2 citations) 6890N gas chromatography-5973 MSD mass spectrometer (2 citations) Mass Selective Detector MSD 5973 mass spectrometer (2 citations) Agilent 5973 inert MSD (2 citations) 6890 GC 5973 N MSD system (2 citations)

21 protocols using msd 5973

Hydrodistillation and GC-MS Analysis of Cupressus sempervirens EO

Check if the same lab product or an alternative is used in the 5 most similar protocols

The EO of dried samples of C. sempervirens aerial parts was hydrodistilled for 3 h by using a Clevenger apparatus. The obtained (CSEO) was collected and dried over anhydrous sodium sulfate and maintained at 4 °C until analysis.
The analysis of the (CSEO) was carried out on a GC/MS HP model 6980 inert MSD, equipped with an Agilent Technologies capillary HP-5MS column (60 m × 0.25 mm, 0.25 mm film thickness) and coupled to a mass selective detector (MSD5973, ionization voltage 70 eV, all Agilent, Santa Clara, CA, USA). The carrier gas was helium and was used at 1.2 mL/min flow rate. The oven temperature program was as follows: 1 min at 100 °C ramped from 100 to 280 °C at 5 °C/min and 25 min at 280 °C. The chromatograph was equipped with a split/split less injector used in the split less mode. Identification of components was appointed by matching their mass spectra with Wiley Registry of Mass Spectral Data 7th edition (Agilent Technologies) and National Institute of Standards and Technology 05 MS (NIST) library data.

Akermi S., Smaoui S., Elhadef K., Fourati M., Louhichi N., Chaari M., Chakchouk Mtibaa A., Baanannou A., Masmoudi S, & Mellouli L. (2022). Cupressus sempervirens Essential Oil: Exploring the Antibacterial Multitarget Mechanisms, Chemcomputational Toxicity Prediction, and Safety Assessment in Zebrafish Embryos. Molecules, 27(9), 2630.

+ Open protocol

+ Expand

GC/MS Analysis of McEO Composition

Check if the same lab product or an alternative is used in the 5 most similar protocols

The analysis of the McEO was performed according to GC/MS HP model 6980 inert MSD (Agilent Technologies, J&W Scientific Products, Palo Alto, CA, USA), equipped with an Agilent Technologies capillary HP-5MS column (60 m length; 0.25 mm i.d; 0.25 mm film thickness), and coupled to a mass selective detector (MSD5973, ionization voltage 70 eV; all Agilent, Santa Clara, CA). The carrier gas was helium and was used at 1.2 mL min⁻¹ flow rate. The oven temperature program was as follows: 1 min at 100 °C ramped from 100 to 280 °C at 5 °C min⁻¹ and 25 min at 280 °C. The chromatograph was equipped with a split/splitless injector used in the splitless mode. Identification of components was assigned by matching their mass spectra with Wiley Registry of Mass Spectral Data 7^th edition (Agilent Technologies, Inc.) and National Institute of Standards and Technology 05 MS (NIST) library data.^{14 (link)}

Ben Hsouna A., Dhibi S., Dhifi W., Mnif W., Ben Nasr H, & Hfaiedh N. (2019). Chemical composition and hepatoprotective effect of essential oil from Myrtus communis L. flowers against CCL4-induced acute hepatotoxicity in rats. RSC Advances, 9(7), 3777-3787.

+ Open protocol

+ Expand

GC-MS Analysis of Volatile Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

GC/MS analysis was carried out in a GC-MS (GC: 6890A, Agilent Technologies, USA; MSD: 5973, Agilent Technologies) using a Factor Four VF 1ms column (25 m, 0.2 mm i.d., 0.33 μm film thickness, Agilent Technologies), as described previously [10 (link)]. Identification was based on the comparison of the retention times and mass spectra of the volatile compounds to Willey/NIST 0.5 and in-house created libraries, as well as on the determination of kovats’ retention indexes (KI) and comparison with those available in the literature.

Mitropoulou G., Nikolaou A., Santarmaki V., Sgouros G, & Kourkoutas Y. (2020). Citrus medica and Cinnamomum zeylanicum Essential Oils as Potential Biopreservatives against Spoilage in Low Alcohol Wine Products. Foods, 9(5), 577.

+ Open protocol

+ Expand

Volatile Compound Analysis of Plant Material

Check if the same lab product or an alternative is used in the 5 most similar protocols

100 g of plant material was sent to the Laboratory of Instrumental Analysis of the Universidad Nacional de Colombia (Medellin Headquarters, Medellin, Colombia). The extraction of the volatile compounds of the vapor phase of the sample was performed through the technique of solid-phase microextraction (SPME), with monitoring in the vapor phase (head space), using a fused silica fiber coated with polydimethylsiloxane–divinyl benzene of 65 μm thickness (PDMSDVB-65 μm, Sigma) [30 (link)].
The chromatographic analysis was performed in a 6890 Series Plus Gas Chromatograph (Agilent Technologies, USA), coupled to a mass selective detector (Agilent Technologies MSD 5973, USA). The column used for the analysis was DB-5MS (5% phenyl poly (methylsilane), 30 m × 0.32 mm x 0.5 μm, Agilent); injection was performed with the SPME device. Identification of secondary metabolites was established based on their mass spectra (EI, 70 eV). The databases used were NIST98.l, NIST02.L, and NIST5a.L from the laboratory in question. Three biological replicates were used, and all analyses were done in duplicate.

Mosquera-Chaverra L., Salas-Moreno M, & Marrugo-Negrete J. (2022). Ethnomedicinal Studies, Chemical Composition, and Antibacterial Activity of the Mammea americana L. Bark in the Municipality of Cértegui, Chocó, Colombia. Advances in Pharmacological and Pharmaceutical Sciences, 2022, 9950625.

+ Open protocol

+ Expand

Cuticular Wax Composition Analysis of BSMV-Infected Leaves

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cuticular wax composition analysis was performed as described [38 (link)]. The leaves (n = 5) with BSMV virus symptoms about 2 weeks post-BSMV-infection were dipped into chloroform (Merck, Rahway, NJ, USA). The extracts dried under N₂ were derivatized at 70 °C for 30 min through reaction with bis-N,O- trimethylsilyl trifluoroacetamide and analyzed with a capillary GC (5890 Series II, Agilent Technologies) and a flame ionization detector (6890 N, Agilent Technologies) with a mass spectrometer (MSD 5973, Agilent Technologies) as previously described [38 (link)]. Quantification was based on FID peak areas relative to the internal standard n-Tetracosane (Merck) and the dry, delipidated tissue weights.

Liu L., Li H., Wang X, & Chang C. (2023). Transcription Factor TaMYB30 Activates Wheat Wax Biosynthesis. International Journal of Molecular Sciences, 24(12), 10235.

+ Open protocol

+ Expand

Terpene-based Cleaning Solution Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The terpene-based cleaning solution was analyzed after experiment using GC-MS. Before injection, 1 ml of the cleaning solution was extracted using 100 ml of dichloromethane (DCM) using a separatory funnel; the bottom layer was then reextracted with 20 ml of DCM twice. This separation procedure was done for two 1-ml portions of the cleaning solution (n = 2). Standards were prepared for α-pinene, β-pinene, limonene, and myrcene by serial dilutions (solvent, DCM; final concentration, ~85 ng ml⁻¹). The samples and standards were then analyzed via GC-MS (GC HP6890, MSD 5973, Agilent) on an HP-5MS capillary column (30 m by 250 μm by 0.25 μm) with helium as the carrier gas and a flow rate of 1.2 ml min⁻¹. The injection volume was 1 μl (splitless mode), and inlet temperature was set to 285°C. The initial oven temperature was set to 70°C for the first 3.5 min, and then it was ramped up at a rate of 30°C min⁻¹; afterward, equilibration time was 6 min. The transfer line temperature was set at 285°C; the temperatures of the MS quad and ion source were 150° and 230°C, respectively. A full scan mode was used to obtain the spectra along the m/z range of 20 to 300.

Rosales C.M., Jiang J., Lahib A., Bottorff B.P., Reidy E.K., Kumar V., Tasoglou A., Huber H., Dusanter S., Tomas A., Boor B.E, & Stevens P.S. (2022). Chemistry and human exposure implications of secondary organic aerosol production from indoor terpene ozonolysis. Science Advances, 8(8), eabj9156.

+ Open protocol

+ Expand

GC-MS Analysis of Chemical Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

An Agilent 6890N Network GC system (Agilent Technologies) equipped with HP-5MS fused silica capillary column (30 m × 0.25 mm i.d. × 0.25 μm film thickness) supplied by Agilent and coupled to a mass selective detector (MSD5973, ionization voltage 70 eV; all Agilent, Santa Clara, France) was used. Helium was used as a carrier gas at 1 ml/min flow rate. The GC oven temperature was held at 60°C for 2 min and then programmed to rise from 60 to 300°C at a rate of 5°C/min. The split/splitless injector (splitless mode) temperature was set to 280°C. The components were identified by careful examination of fragmentation patterns [15 ] and spectral data obtained from the Wiley and NIST libraries. Determination was carried out in duplicate.

Zouari Bouassida K., Bardaa S., Khimiri M., Rebaii T., Tounsi S., Jlaiel L, & Trigui M. (2017). Exploring the Urtica dioica Leaves Hemostatic and Wound-Healing Potential. BioMed Research International, 2017, 1047523.

+ Open protocol

+ Expand

Lippia citriodora Oil Extraction Protocol

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Collection and identification of plant material was described in a previous study by our team regarding the biological properties of Lippia citriodora oil [27 (link)]. Briefly, plant samples of small shrubs were purchased by Vioryl S.A. from an herbal market (Afidnes, Athens, Greece), identified as Lippia citriodora by a botanist, and planted and maintained in pots until their first inflorescence in May/June when the leaves and stems were collected. The collected parts of the herb were subjected to hydrodistillation in a laboratory-scale, water-steam distillation apparatus as described in our team’s previously published study [27 (link)]. The composition of the extracted oil was analyzed by Gas chromatography–mass spectrometry (GC-MS) (GC: 6890 A, Agilent Technologies, USA; MSD: 5973, Agilent Technologies, Santa Clara, CA, USA) using an HP-1 ms column (25 m, 0.2 mm i.d., 0.33 μm film thickness). The detailed analysis process and chemical composition of the oil are described in Fitsiou et al. [27 (link)].

Spyridopoulou K., Aravidou T., Lampri E., Effraimidou E., Pappa A, & Chlichlia K. (2021). Antitumor Potential of Lippia citriodora Essential Oil in Breast Tumor-Bearing Mice. Antioxidants, 10(6), 875.

+ Open protocol

+ Expand

Metabolite Extraction and Analysis of Leaf Samples

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Leaf samples (1 g), ground with liquid nitrogen, were sequentially extracted with hexane, diethyl ether, and methanol. For the determination of trehalose, raffinose, and myo-inositol, the residue obtained after extraction in diethyl ether was further extracted with methanol and dried under vacuum at 90 °C. The pellets were resuspended in 0.5 cm³ pyridine, and the metabolites were derivatized by adding 0.05 cm³ N,O-bis(trimethylsilyl)trifluoroacetamide containing 1% trimethylchlorosilane. Extracts were analyzed with an HP 6890 Gas Chromatograph equipped with a mass selective detector MSD 5973 (Agilent Technologies, Santa Clara, CA, USA), with an electronic autosampler 7693A ALS system, electronic pressure control, and split/splitless injector (Agilent Technologies, Santa Clara, CA, USA). The injector worked in a split 1:50 mode at 250 °C. The volume of the sample introduced into the injector was 1 μL. The transfer line temperature was 280 °C. Separation was performed on HP-5ms (30 m × 0.25 mm; 0.25 μm film thickness) fused silica column, with a helium flow rate of 1 cm³ min^–1 [1 (link)].

Kopczewski T., Kuźniak E., Ciereszko I, & Kornaś A. (2022). Alterations in Primary Carbon Metabolism in Cucumber Infected with Pseudomonas syringae pv lachrymans: Local and Systemic Responses. International Journal of Molecular Sciences, 23(20), 12418.

+ Open protocol

+ Expand

Quantification of Pyrroles via GC-MS Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pyrroles were quantitated using an Agilent 6890N gas chromatograph coupled to a mass spectrometer (MSD 5973, Agilent Technologies Inc., Santa Clara, CA). Two μL samples of organic extract were injected in splitless mode (injector temperature, 240 °C; splitless time, 0.75 min) on a BP20 capillary column (50 m × 0.22 mm, 0.25 mm film thickness, SGE, Courtaboeuf, France). The carrier gas was helium N60 (Air Liquide) with a flow rate of 1 mL/min. The oven was programmed at 45 °C for the first minute, heated to 185 °C at 3°C/min, then raised to 240 °C at 10 °C/min, and held at this temperature for 20 min. The transfer line between GC and MS was set at 250 °C and the ion source at 230 °C. The mass spectrometer was operated in electron ionization mode at 70 eV in selected-ionmonitoring (SIM) mode. Monitored ions are listed in Table 2. Quantitation was performed with calibration curves built using white wine or oak wood extract.

Gammacurta M., Laboyrie J., Prida A., Lavigne V., Moine V., Darriet P, & Marchal A. (2021). Contribution of Grapes and Oak Wood Barrels to Pyrrole Contents in Chardonnay Wines: The Influence of Several Cooperage Parameters. Journal of agricultural and food chemistry, 69(29).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!