Cells were lysed and subjected to western blot analysis as previously described [17 (link)]. Briefly, cells were lysed in sodium dodecyl sulfate (SDS) lysis buffer (2 % SDS, 10 % glycerol, 62 mM Tris-HCl, pH 6.8, and a complete mini-protease inhibitor cocktail [Roche Life Science]) and boiled for 10 min. After protein quantification, equal amount of protein was loaded, separated on a polyacrylamide gel, and transfering to a nitrocellulose membrane. Protein bands were immunodetected with appropriate antibodies, e.g., rabbit-anti- NEDD4L (Cell Signaling, Danvers, MA, Cat. No. 4013, 1:1000), mouse anti-E-cadherin (BD Transduction Laboratories, San Jose, CA, Cat. No. 610181, 1:1000), mouse antivimentin (Cell Signaling, Cat. No. 3390, 1:1000), rabbitanti-pSMAD2 (Cell Signaling, Cat. No. 9101, 1:1000), mouse anti-GAPDH (Santa Cruz Biotechnology, Dallas, TX, Cat. No. sc-47724, 1:4000), goat anti-actin (Santa Cruz Biotechnology, Cat. No. sc-1616, 1:1000), and their corresponding secondary antibodies (Santa Cruz Biotechnology).
Rabbit anti nedd4l
Manufactured by Cell Signaling Technology
Rabbit-anti-NEDD4L is a primary antibody that specifically recognizes the NEDD4L (neural precursor cell expressed, developmentally down-regulated 4-like) protein. NEDD4L is an E3 ubiquitin-protein ligase that plays a role in the regulation of various cellular processes. This antibody can be used for the detection and analysis of NEDD4L in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti actin, by Santa Cruz Biotechnology (1 mentions)
Mouse anti gapdh, by Santa Cruz Biotechnology (1 mentions)
Mouse anti e cadherin, by BD (1 mentions)
Complete mini protease inhibitor cocktail, by Roche (1 mentions)
Rabbit anti p smad2, by Cell Signaling Technology (1 mentions)
Mouse anti vimentin, by Cell Signaling Technology (1 mentions)
Rabbit anti actin, by Merck Group (1 mentions)
Mouse anti myc, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti mtor, by Cell Signaling Technology (1 mentions)
Mouse anti tubulin, by Merck Group (1 mentions)
Rabbit anti ulk1, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti beclin1, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti p62, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti lc3, by Cell Signaling Technology (1 mentions)
2 protocols using rabbit anti nedd4l
1
Comprehensive Western Blot Analysis
2
Antibody Validation for Autophagy Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The primary antibodies used in this study were: mouse and rabbit anti–HA tag antibody (Sigma-Aldrich), mouse anti-Myc (Santa Cruz Biotechnology, Inc.), rabbit anti–BECLIN 1 (Santa Cruz Biotechnology, Inc.), rabbit anti-LC3 (Cell Signaling Technology), rabbit anti–human AMBRA1 (Novus Biologicals), mouse anti-multiubiquitin (MBL), rabbit anti-ULK1 (Santa Cruz Biotechnology, Inc.), goat anti-ULK1 (Santa Cruz Biotechnology, Inc.), rabbit anti-pS757 ULK1 (Cell Signaling Technology), mouse anti-ULK2 (Abcam), rabbit anti-NEDD4L (Cell Signaling Technology), mouse anti-NEDD4L (Santa Cruz Biotechnology, Inc.), rabbit anti-pS342 NEDD4L (Cell Signaling Technology), rabbit anti-mTOR (Cell Signaling Technology), rabbit p-S2448 mTOR (Cell Signaling Technology), rabbit anti-p62 (Santa Cruz Biotechnology, Inc.), rabbit anti-ATG13, rabbit p-S318 ATG13 (Rockland), rabbit anti-Actin (Sigma-Aldrich), mouse anti-Tubulin (Sigma-Aldrich), rabbit cleaved-PARP (Cell Signaling Technology), rabbit anti-Atg16L (Cell Signaling Technology), and rabbit p-p70S6K and p70S6K (Cell Signaling).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!