The hot carcass weight (HCW) was determined prior to chilling (24 h at 4 °C). After the cooling process, the cold carcass weight (CCW) was measured, including the kidneys and internal fat. Carcass D% was calculated as = ([CCW/EBW] × 100), and the percentage of cooling loss (CL%) = ([HCW-CCW]/HCW) × 100. After a 24 h chilling period, the carcass dimensions were measured using a flexible tape measure, including carcass length, leg length, and chest circumference. Longissimus muscle area (LMA) and FT between the 12th and 13th ribs were assessed on days 0, 14, 28, and 42, employing an Aloka Prosound 2 instrument with a 3.5 MHz linear transducer.
Prosound 2
Manufactured by Aloka
Sourced in Japan
The ProSound 2 is a diagnostic ultrasound system designed for medical professionals. It features a high-resolution display and intuitive controls for image acquisition and analysis.
Automatically generated - may contain errors
Lab products found in correlation
7 protocols using prosound 2
1
Carcass Evaluation and Chilling Effects
2
Gestational Fat Thickness Monitoring
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In both farms, all measurements of fat thickness (FAT), expressed in centimeters (cm), were taken two weeks apart from the beginning of the last trimester of gestation to around 80 days postpartum (dpp), using the ALOKA ProSound 2 ultrasound equipment with a convex array transducer 3.5 MHz. The transducer was placed horizontally in the caudal region between the iliac and the ischial tuberosity [11 (link)].
3
Measuring Muscle Thickness and Volume
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Muscle thickness of knee extensors (KE) and ankle plantar flexors (PF) were measured with a B-mode ultrasonographic apparatus (Prosound2, Aloka, Tokyo, Japan) with a linear scanner. As described in the earlier study [34 (link)], the ultrasonographic images were obtained at 50% of femur length (the distance from the greater trochanter of the femur to the articular cleft between the femur and the tibial condyles) and the proximal 30% of lower leg length (the distance from the tibial condyles and lateral malleolus). The muscle thickness (MT) was defined as the distance from adipose tissue-muscle and bone interface. The muscle volume indices (MV) of the knee extensors and ankle plantar flexors were calculated using the prediction equations derived from MT and limb length (L) reported by Miyatani et al. [35 (link)]:
and muscle quality was expressed as TQ relative to MV (TQ/MV) [18 (link)] in each muscle.
and muscle quality was expressed as TQ relative to MV (TQ/MV) [18 (link)] in each muscle.
4
Measurement of Muscle Thickness Using Ultrasound
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MT was assessed by transverse B-mode ultrasound images taken using a portable ultrasound system (ProSound 2, ALOKA, Japan) with a 9.0 MHz linear probe and saved in a computer. Measures were taken at the mid portion of the upper arm, at half-distance between the acromion process of the clavicle to the lateral epicondyle of the humerus. The probe was positioned perpendicularly to the limb and coated with a generous amount of water-soluble transmission gel to provide acoustic contact between the skin and the transducer. All images were collected and analyzed with caution by the same investigator, avoiding compression of the dermal surface. Three measurements were performed. MT was determined using a computer with ImageJ 1.42q software (National Institutes of Health, Bethesda, Maryland). The mean of the three measurements of MT was considered for analysis.
5
Ultrasound Measurement of Bovine Subcutaneous Fat
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All measurements of fat thickness (FAT), expressed in centimeters (cm), were taken two weeks apart, from the beginning of the last trimester of gestation to 90 ± 6 d PP, using the ALOKA ProSound 2 ultrasound equipment with a convexed array transducer 3.5 MHz. The FAT was measured in the thurl area located midway between the tuber coxae (hooks) and the tuber ischiae (pins), 2 to 3 cm above the greater trochanter of the femur (Schröder and Staufenbiel, 2006 (link)). Body condition score was evaluated at the onset of the study by observation using the scale 1 to 9 (1= extremely thin, 9= very fat) as indicated by Wagner et al. (1988) (link).
6
Ultrasound Pregnancy Monitoring in Goats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pregnancy was diagnosed by ultrasonography (US) (Prosound 2, ALOKA, Tokyo, Japan) on day 23 post NM using a multi-frequency linear trans-rectal probe (UST 660-7.5, ALOKA, Tokyo, Japan) and confirmed on day 35 and 60 using a multi frequency convex abdominal probe (UST-9137C, ALOKA, Tokyo, Japan). Number and sex of embryos were recorded. All pregnancies were tracked until kidding and the number and sex of kids were confirmed.
7
Follicular Wave Synchronization in Cows
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cows satisfying the inclusion criteria received the following synchronization protocol to initiate a new follicular wave. On day 1, the cows received an intravaginal progesterone-releasing device (IPD; CIDR® [1.38 g of progesterone], Zoetis, Quito, Ecuador) + 2 mg of intramuscular estradiol benzoate (EB; Gonadiol®, Zoetis). Seven days later, in the morning, an intramuscular dose of 25 mg of prostaglandin (PG; Lutalyse® [dinoprost tromethamine], Zoetis) was applied and the implant was removed. On the following day (day 8), the cows received 1 mg of intramuscular EB (Gonadiol®) and, 54 h after removal of the implant, the location of the preovulatory follicle was explored by transrectal ultrasound (Aloka ProSound 2® , Tokyo, Japan) using a 7.5-MHz linear transducer. On day 11, which was considered the day of re-initiation of the follicular wave or 'day 0' of the study, the coccygeal vein was punctured, and blood was sampled into evacuated tubes containing ethylenediaminetetraacetic acid (BD Vacutainer®, Franklin Lakes, NJ, USA). Samples were immediately placed in a cooler with a cooling gel at 5 °C and centrifuged in the laboratory at 1500 g for 20 min. The plasma obtained was stored in aliquots at -20 °C until subsequent analysis of AMH concentrations. On day 11, AFC was also performed (the design is shown in online Supplementary Fig. S1).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!