Uplfln 40x oil immersion objective

The microscope slides containing the stained samples were imaged using an UPLFLN 40x oil immersion objective (Olympus America, Center Valley, PA) and digital CCD camera ORCA-R2 C10600-10B (Hamamatsu Photonics, Shizuoka Prefecture, Japan) mounted on an IX-83 inverted motorized microscope (Olympus America, Center Valley, PA). Fluorescence images of ten randomly selected fields of view per slide were taken at 40x magnification (6.22 pixels/μm resolution) for each sample.

The samples were mounted at 90° and imaged with an IX-83 inverted motorized microscope (Olympus America, Center Valley, PA, USA). Images were taken using an UPLFLN 40x oil immersion objective (Olympus America, Center Valley, PA, USA) and a digital CCD camera ORCA-R2 C10600-10B (Hamamatsu Photonics, Shizuoka Prefecture, Japan). Ten fields of views randomly selected for each sample and imaged at 40x magnification (6.22 µm/pixel). Connexin 43 images were obtained with the Olympus Fluoview FV3000 microscope (Olympus America, Center Valley, PA, USA) also using a UPLSAPO 40x silicone oil immersion objective (Olympus America, Center Valley, PA, USA).