Pitavastatin (Livalo, Adooq), zoledronic acid and risedronate (Selleck), and GGTI-2133, Tipifarnib, farnesol, geranylgeraniol and mevalonate (Sigma-Aldrich) were prepared as 20 mM solutions in DMSO except zoledronic acid which was dissolved in H2O.
Tipifarnib
Manufactured by Merck Group
Sourced in United States
Tipifarnib is a lab equipment product manufactured by Merck Group. It is a farnesyltransferase inhibitor that functions by blocking the activity of the enzyme farnesyltransferase, which is involved in the post-translational modification of certain proteins.
Automatically generated - may contain errors
Lab products found in correlation
Zoledronic acid, by Merck Group (2 mentions)
Farnesol, by Merck Group (1 mentions)
Zoledronic acid, by Selleck Chemicals (1 mentions)
Geranylgeraniol, by Merck Group (1 mentions)
Ggti 2133, by Merck Group (1 mentions)
Mevalonate, by Merck Group (1 mentions)
Tipifarnib, by MedChemExpress (1 mentions)
Lonafarnib, by Merck Group (1 mentions)
Adagrasib, by MedChemExpress (1 mentions)
Sotorasib, by MedChemExpress (1 mentions)
Sw1573, by American Type Culture Collection (1 mentions)
Ketotifen fumarate, by Merck Group (1 mentions)
Dasatinib, by Merck Group (1 mentions)
Lovastatin, by Abcam (1 mentions)
Mevalonic acid, by Merck Group (1 mentions)
Tipifarnib, by Selleck Chemicals (1 mentions)
Male c57bl 6n mice, by Charles River Laboratories (1 mentions)
Concanavalin a (cona), by Merck Group (1 mentions)
5 protocols using tipifarnib
1
Compound Preparation for Experimental Studies
2
Evaluating KRAS-G12C Inhibitors in Cancer
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Three KRAS-G12C mutant lung adenocarcinoma, one colorectal and one pancreatic cancer cell line were involved in this study shown in Supplementary Table 1 . H358 and SW1573 were purchased from ATCC. PF97 and PF139 cells were established from malignant pleural effusion samples in cooperation with the West German Biobank Essen as described earlier [16 (link)]. The patients provided written informed consent and the experiments were approved by the Ethics Committee of the University Hospital Essen (#18-8208-BO).
Lonafarnib and tipifarnib (for in vitro experiments) were purchased from Sigma (St. Louis, MO, USA), while sotorasib, adagrasib and tipifarnib (for in vivo experiments) were obtained from Medchemexpress. For in vitro experiments, drugs were dissolved in dimethyl sulfoxide (DMSO) in 10 mM stock concentration and stored at −80 °C.
Lonafarnib and tipifarnib (for in vitro experiments) were purchased from Sigma (St. Louis, MO, USA), while sotorasib, adagrasib and tipifarnib (for in vivo experiments) were obtained from Medchemexpress. For in vitro experiments, drugs were dissolved in dimethyl sulfoxide (DMSO) in 10 mM stock concentration and stored at −80 °C.
3
Preparation and Application of Chemical Inhibitors
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U18666A was acquired from Merck, Ketotifen-fumarate, and Tipifarnib were bought from Sigma-Aldrich. The substances were dissolved in DMSO to produce stock solutions (U18666A at 4 mg/ml, Ketotifen at 20 mM, and Tipifarnib at 5 mM), which were subsequently aliquoted and frozen at -80°C. The drugs were added to the complete growth medium at the indicated time points and at the concentration indicated for each experiment. The medium containing the inhibitor was renewed every 24 hours.
4
In vitro Compound Solubilization Protocol
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Compounds used for in vitro experiments were commercially available and listed below. Fluvastatin (abcam, ab120651), cerivastatin (abcam, ab142853), trametinib (biomol, GSK-1120212), mevalonic acid (sigma-aldrich, 50838), lovastatin (abcam, ab120614), dasatinib (Sigma-Aldrich, SML2589), tipifarnib (Sigma-Aldrich, SML1668) were solved in DMSO (AppliChem, A3672). The final concentration of DMSO in medium was maximal 1% and DMSO only was used as vehicle control at the same final concentration. Zoledronic acid (Sigma-Aldrich, SML0223) was solved in water (Braun).
5
Concanavalin A-Induced Liver Injury Model
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Male C57BL/6N mice (8-10 weeks of age and weighing 20-25 g) were purchased from Charles River Laboratories Japan, Inc. (Yokohama, Japan). The mice were maintained in controlled chambers (22 °C ± 2 °C and 12-h light/dark cycle) and provided with water and food ad libitum. The Ethics Committee for Animal Experiments in Kyushu University approved all animal experiments (approval number A30-117-0). All methods were performed in accordance with the Guidelines for Animal Experiments of Kyushu University.
In this study, mice were randomly divided into three groups: a sham, PBS, and tipifarnib group. Mice in the sham and PBS groups were initially administrated 10 ml/kg phosphate-buffered saline (PBS) intraperitoneally. Mice in the tipifarnib group were initially subjected to intraperitoneal injection with tipifarnib (10 mg/kg, Selleckchem, Houston, TX). At 1 h after PBS or tipifarnib administration, the mice in tipifarnib or PBS groups were administered 20 mg/kg Con A (Sigma-Aldrich, USA) and 10 ml/kg normal saline; or the mice in shame group were administered 20 mg/kg PBS and 10 ml/kg normal saline. tipifarnib was dissolved in DMSO and then mixed with PBS.
In this study, mice were randomly divided into three groups: a sham, PBS, and tipifarnib group. Mice in the sham and PBS groups were initially administrated 10 ml/kg phosphate-buffered saline (PBS) intraperitoneally. Mice in the tipifarnib group were initially subjected to intraperitoneal injection with tipifarnib (10 mg/kg, Selleckchem, Houston, TX). At 1 h after PBS or tipifarnib administration, the mice in tipifarnib or PBS groups were administered 20 mg/kg Con A (Sigma-Aldrich, USA) and 10 ml/kg normal saline; or the mice in shame group were administered 20 mg/kg PBS and 10 ml/kg normal saline. tipifarnib was dissolved in DMSO and then mixed with PBS.
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