4 4 azobis 4 cyanovaleric acid acva

2-methacryloyloxyethyl phosphorylcholine (MPC, 97%), L-serine (≥99.5%), sodium phosphotungstate hydrate, sodium carbonate (≥99.5%), TWEEN 80, 4-cyano-4-(phenylcarbonothioylthio) pentanoic acid (CPD, 97%), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldric. HPLC-grade tetrahydrofuran and chloroform, acetone, deuterium oxide, and deferoxamine mesylate (DFO, 95%) were purchased from Acros Organics. 4,4’-azobis (4-cyanovaleric acid) (ACVA, 98%), curcumin (95%), acryloyl chloride (55%), and iron (III) chloride hexahydrate were purchased from Alfa Aesar. Nanosep centrifugal devices with Omega membrane (3K MWCO) were purchased from Pall Corporation. Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), and (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) (MTT) assay kits were purchased from Thermo Fisher Scientific. Dialysis membranes (8K MWCO) were purchased from Spectrum Laboratories, Inc. Copper mesh was purchased from Ted Pella. Copper sulfate was purchased from Showa Co., Ltd. MDA-MB-231 breast cancer cells were purchased from the Bioresource Collection and Research Center in Taiwan. Deionized water with a minimum resistivity of 18.0 MΩ·m was used in experiments.