D glucose 6 phosphate sodium salt

Manufactured by Merck Group

Sourced in United States

D-glucose 6-phosphate sodium salt is a chemical compound used in various laboratory applications. It serves as a substrate or cofactor in enzymatic reactions and is commonly used in biochemical research and analysis. The product is a white, crystalline powder that is soluble in water.

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Close spelling variants of this product

Glucose 6-phosphate sodium salt D-Glucose-6-phosphate Phosphate salts D-glucose D-(+)-glucose (glucose); Sodium phosphate

8 protocols using d glucose 6 phosphate sodium salt

Enzymatic Assay for Screening 3-Nitro-2-Phenyl-2H-Chromene Analogues

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Adenosine 5′-triphosphate disodium salt hydrate (≥99%), chlorophenol red-β-d-galactopyranoside (CPRG), β-nicotinamide adenine dinucleotide phosphate hydrate (NADP⁺), magnesium chloride hexahydrate (≥99%), d-(+)-glucose (≥99%), dimethyl sulfoxide (ACS reagent (≥99.9%)), d-glucose-6-phosphate sodium salt, N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) (HEPES, ≥99.5%), imidazole (96.0%), Terrific broth modified, and triethanolamine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from CellGro Technologies, LLC (Lincoln, NE, USA). Leuconostoc mesenteroides glucose-6-phosphate dehydrogenase (LmG6PDH) was purchased from Worthington Biochemical Corporation (Lakewood, NJ, USA). NADPH tetrasodium salt (≥95%) was purchased from Enzo Life Sciences, Inc. (Farmingdale, NY, USA). For the 3-nitro-2-phenyl-2H-chromene analogues, compounds 1–7 were purchased from TimTec, LLC (Tampa, FL, USA), while compounds 8–13 were purchased from ChemDiv, Inc. (San Diego, CA, USA). Ethylenediaminetetraacetic acid (EDTA) tetrasodium salt hydrate (98%), Luria-Bertani (LB) broth, lysozyme (type VI), protease inhibitor tablets (EDTA-free), glycerol, and all other chemicals were purchased from Fisher Scientific (Hampton, NH, USA).

Carey S.M., O’Neill D.M., Conner G.B., Sherman J., Rodriguez A, & D’Antonio E.L. (2024). Discovery of Strong 3-Nitro-2-Phenyl-2H-Chromene Analogues as Antitrypanosomal Agents and Inhibitors of Trypanosoma cruzi Glucokinase. International Journal of Molecular Sciences, 25(8), 4319.

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CYP1A1, CYP1A2, and CYP1B1 Supersomes Assay

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Gentest human CYP1A1, CYP1A2, and CYP1B1 supersomes were purchased from BD Biosciences (Franklin Lakes, NJ). D-Glucose 6-phosphate sodium salt, β-nicotinamide adenine dinucleotide phosphate sodium salt (NADP⁺), and glucose 6-phosphate dehydrogenase were purchased from Sigma-Aldrich Corporation. Other reagents in bioassays were purchased from Fisher Scientific International, Inc. Figures were plotted with Prism 6 (GraphPad Software, Inc., La Jolla, CA) as well as Microsoft Excel 2013.

Liu J., Pham P.T., Skripnikova E.V., Zheng S., Lovings L.J., Wang Y., Goyal N., Bellow S.M., Mensah L.M., Chatters A.J., Bratton M.R., Wiese T.E., Zhao M., Wang G, & Foroozesh M. (2015). A Ligand-Based Drug Design. Discovery of 4-Trifluoromethyl-7,8-pyranocoumarin as a Selective Inhibitor of Human Cytochrome P450 1A2. Journal of medicinal chemistry, 58(16), 6481-6493.

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Isotopic DHFR Preparation for NMR

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Reduced β-nicotinamide adenine
dinucleotide phosphate tetrasodium salt hydrate (NADPH), d-glucose-6-phosphate sodium salt, and glucose-6-phosphate dehydrogenase
from Leuconostoc mesenteroides were
purchased from Sigma-Aldrich. (6S)-5,6,7,8-Tetrahydrofolic
acid (THF) was obtained from Schircks Laboratories. Expression and
purification of L28F DHFR were performed as described previously.^{6 (link),26 (link)} Isotopically labeled L28F DHFR for proton relaxation dispersion
measurements was expressed in M9 medium containing 0.5 g/L of ¹⁵NH₄Cl, 0.5 g/L ¹⁵NH₄(SO₄)₂, and 3 g/L [¹³C,¹H]-glucose
in 99% D₂O according to published protocols.^{27 (link),28 (link)}

Oyen D., Fenwick R.B., Aoto P.C., Stanfield R.L., Wilson I.A., Dyson H.J, & Wright P.E. (2017). Defining the Structural Basis for Allosteric Product Release from E. coli Dihydrofolate Reductase Using NMR Relaxation Dispersion. Journal of the American Chemical Society, 139(32), 11233-11240.

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Quantitative Analysis of Metabolites

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Enniatin B1 (ENNB1), deoxynivalenol (DON), midazolam (MDZ) and 4-hydroxymidazolam (4-OH-MDZ), mevastatine and bromocriptine (BCT), NADP⁺, NADPH, D-glucose 6-phosphate sodium salt, D-glucose 6-phosphate dehydrogenase from baker’s yeast (Saccharomyces cerevisiae), HEPES buffer, and ammonium formate (NH₄COOH) were purchased from Sigma-Aldrich (Millipore Sigma; St. Louis, MO, USA). Troleandomycin (TAO) was supplied by Santa Cruz Biotechnology, Inc. (Dallas, Texas, TX, USA). Atorvastatin (ARVS) lactone and the metabolites 4-OH-ATVS lactone and 2-OH-ATVS lactone were from Toronto Research Chemicals (North York, ON, Canada), and cortexolone was from Steraloids (Newport, RI, USA). Progesterone (PGS) and the metabolite 6β-OH-PGS were from Clearsynth (Mississauga, Ontario, Canada). Fluka (Millipore Sigma; St. Louis, MO, USA) supplied magnesium chloride hexahydrate (MgCl₂ × 6 H₂O). With exception of DON that was dissolved in 90% acetonitrile (MeCN), all of the stock solutions were prepared in HPLC grade methanol (MeOH).
Fisher Scientific provided chromatographic solvents, including MeCN, MeOH, and water (Optima, LC/MS grade) (Waltham, MA, USA). Formic acid (HCOOH) was purchased from Merck (Darmstadt, Germany).

Ivanova L., Denisov I.G., Grinkova Y.V., Sligar S.G, & Fæste C.K. (2019). Biotransformation of the Mycotoxin Enniatin B1 by CYP P450 3A4 and Potential for Drug-Drug Interactions. Metabolites, 9(8), 158.

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Comprehensive Biochemical Assay Protocol

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1-Octanol, hydrogen peroxide, magnesium chloride,
acetic acid, phosphoric acid, triethylamine, propranolol, atenolol,
dimethyl sulfoxide (DMSO), d-glucose 6-phosphate sodium salt,
nonessential amino acids (NEAA), glucose-6-phosphate dehydrogenase
(G-6-PDH), β-nicotinamide adenine dinucleotide phosphate sodium
salt (NADP⁺), verapamil, MK-571, testosterone, sodium phosphate
monobasic monohydrate, sodium phosphate dibasic, Pd(PPh₃)₂Cl₂, CuI, PPh₃, i-Pr₂NH, toluene, and ethynylbenzene were purchased from Sigma (St. Louis,
MO). Sodium pyruvate, Hank’s Balanced Salt Solution (HBSS),
and Phosphate-Buffered Saline (PBS) were from Cellgro (Manassas, VA).
Hepes, glutamine, fetal bovine serum (FBS), and penicillin/streptomycin
were from Invitrogen (St. Louis, MO). NaCl, NH₄Cl, CH₂Cl₂, sodium sulfate, formalin, hydrochloric acid,
paraffin phosphate buffer, HPLC grade-acetonitrile, ethyl acetate,
ethanol, and methanol were from Fisher Scienfitic Co. (Pittsburgh,
PA). Polyethylene glycol 400 (PEG) was from Hampton (Aliso Viejo,
CA).

Kong H., Song J.K., Yenugonda V.M., Zhang L., Shuo T., Cheema A.K., Kong Y., Du G.H, & Brown M.L. (2014). Preclinical Studies of the Potent and Selective Nicotinic α4β2 Receptor Ligand VMY-2-95. Molecular Pharmaceutics, 12(2), 393-402.

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Cytochrome P450 Enzyme Assay

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Gentest human
CYP1A1, CYP1A2,
CYP1B1, and CYP2A6 supersomes and rat CYP2B1 supersomes were purchased
from BD Biosciences (Franklin Lakes, NJ). d-Glucose-6-phosphate
sodium salt, β-nicotinamide adenine dinucleotide phosphate sodium
salt (NADP⁺), and glucose-6-phosphate dehydrogenase were
purchased from Sigma-Aldrich Corporation. Other reagents in bioassays
were purchased from Fisher Scientific International, Inc. Figures
were plotted with Prism 6 (GraphPad Software, Inc., La Jolla, CA).

Goyal N., Liu J., Lovings L., Dupart P., Taylor S., Bellow S., Mensah L., McClain E., Dotson B., Sridhar J., Zhang X., Zhao M, & Foroozesh M. (2014). Ethynylflavones, Highly Potent, and Selective Inhibitors of Cytochrome P450 1A1. Chemical Research in Toxicology, 27(8), 1431-1439.

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Quantitative Analysis of Testosterone Metabolites

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Acetic acid, ethanol, NaCl, potassium dihydrogen phosphate, dibasic potassium phosphate, monobasic sodium phosphate, dibasic heptahydrate sodium phosphate, potassium sodium tartrate tetrahydrate, glycerol, Folin & Ciocalteu’s phenol reagent, albumin from bovine serum, anhydrous sodium carbonate, NADP, D-glucose 6-phosphate sodium salt, glucose-6-phosphate dehydrogenase, MgCl, 1-chloro-2,4-dinitrobenzene, reduced L-glutathione and testosterone were purchased from Sigma-Aldrich Merck (Saint Quentin Fallavier, France). Acetonitrile and methanol (high-performance liquid chromatography (HPLC) grade) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Ammonium acetate, sodium hydroxide and copper sulfate were purchased from VWR (Fontenay-sous-Bois, France). Flo-Scint™ II and Ultima Gold™ liquid scintillation cocktails were purchased from PerkinElmer (Courtabœuf, France). Ultrapure water produced by a Milli-Q system (Millipore, Saint-Quentin-en-Yvelines, France) was used for the preparation of HPLC mobile phases. [¹⁴C]-testosterone (specific activity: 2.18 GBq/mmol; 98.8% pure) was purchased from PerkinElmer. Standards of testosterone metabolites were purchased from Steraloids (Newport, RI, USA) or from Sigma-Aldrich.

Barretto S.A., Lasserre F., Huillet M., Régnier M., Polizzi A., Lippi Y., Fougerat A., Person E., Bruel S., Bétoulières C., Naylies C., Lukowicz C., Smati S., Guzylack L., Olier M., Théodorou V., Mselli-Lakhal L., Zalko D., Wahli W., Loiseau N., Gamet-Payrastre L., Guillou H, & Ellero-Simatos S. (2021). The pregnane X receptor drives sexually dimorphic hepatic changes in lipid and xenobiotic metabolism in response to gut microbiota in mice. Microbiome, 9, 93.

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Reagents and Chemicals Used in Research

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CDDO-Me was purchased from Toronto Research Chemicals (North York, ON). CDDO-Im was purchased from Tocris (Bristol, United Kingdom). Bovine serum albumin, Tween-20, flavin adenine dinucleotide, 3,3′-methylene-bis (dicoumarol), D-glucose 6-phosphate sodium salt, glucose-6-phosphate dehydrogenase, thiazolyl blue tetrazolium bromide (MTT), and ConA were purchased from Sigma (St. Louis, MO). 2-Methyl-1,4-naphthoquinone (menadione) and digitonin were purchased from Acros (Antwerp, Belgium), 15Δ-PGJ₂ from Cayman Chemical (Ann Arbor, MI), and CdCl₂ from Thomas Scientific (Swedesboro, NJ). Tris, NADP, and EDTA were purchased from BioRad (Hercules, CA), Roche (Indianapolis, IN), and Thermo Fisher Scientific (Waltham, MA), respectively.

Gatbonton-Schwager T., Yagishita Y., Joshi T., Wakabayashi N., Srinivasan H., Suzuki T., Yamamoto M, & Kensler T.W. (2023). A Point Mutation at C151 of Keap1 of Mice Abrogates NRF2 Signaling, Cytoprotection in Vitro, and Hepatoprotection in Vivo by Bardoxolone Methyl (CDDO-Me). Molecular Pharmacology, 104(2), 51-61.

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