The immortalized normal human urothelial cell line SV-HUC-1 and human bladder urothelial carcinoma (BLCA) cell lines T24 and 5637, and human proximal tubular epithelial cell line HK-2 and human clear cell renal cell carcinoma (ccRCC) cell lines 786-O and caki-1, and human normal prostatic epithelial cells RWPE-1 and human prostate cancer (PCa) cell lines PC-3 and DU145 were all purchased from Procell Life Science and Technology Co., Ltd. (Wuhan, China). SV-HUC-1 and PC-3 cells were cultured in Ham’s F-12K medium (Procell) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. T24, HK-2, caki-1, and DU145 cells were cultured in DMEM medium (Procell) supplemented with 10% FBS and 1% penicillin/streptomycin. RWPE-1 cells were cultured in complete medium of human prostatic epithelial cells (Procell). Furthermore, 5637 and 786-O cells were cultured in RPMI 1640 medium (Procell) supplemented with 10% FBS and 1% penicillin/streptomycin. All the cell lines were cultured in an incubator with 5% CO2 at 37 °C. Elesclomol (Synta Pharmaceuticals, Lexington, MA, United States) was diluted in a cell culture medium at 100 nmol/L concentration.
Rwpe 1
Manufactured by Procell
Sourced in Israel, China
RWPE-1 is a human prostate epithelial cell line derived from normal prostate tissue. The cells are adherent and exhibit a cobblestone-like morphology. RWPE-1 cells can be used for in vitro studies related to prostate biology and function.
Automatically generated - may contain errors
Lab products found in correlation
Du145, by Procell (5 mentions)
Dmem medium, by Procell (2 mentions)
Sv huc 1, by Procell (2 mentions)
Rpmi 1640 medium, by Procell (2 mentions)
Caki 1, by Procell (2 mentions)
Ham s f 12k medium, by Procell (2 mentions)
Rpmi 1640 medium, by Sartorius (1 mentions)
Rwpe 1, by Sartorius (1 mentions)
Mem medium, by Sartorius (1 mentions)
Du145, by Sartorius (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Lncap, by Procell (1 mentions)
7 protocols using rwpe 1
1
Cell Culture Conditions for Cancer and Normal Cell Lines
2
Culturing Human Cell Lines for Research
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The human proximal tubular epithelial cell line HK-2 and human ccRCC cell lines 786-O and Caki-1, immortalized normal human urothelial cell line SV-HUC-1 and human bladder urothelial carcinoma cell lines T24 and 5637, and human normal prostatic epithelial cell line RWPE-1 and human PCa cell lines PC-3 and DU145 were all purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China). HK-2, Caki-1, T24, and DU145 cells were cultured in DMEM medium (Procell) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Furthermore, 786-O and 5637 cells were cultured in RPMI 1640 medium (Procell) supplemented with 10% FBS and 1% penicillin/streptomycin. SV-HUC-1 and PC-3 cells were cultured in Ham’s F-12K medium (Procell) supplemented with 10% FBS and 1% penicillin/streptomycin. RWPE-1 cells were cultured in complete medium of human prostatic epithelial cells (Procell). All the cell lines were cultured in an incubator with 5% CO2 at 37 °C.
3
Isolation and Characterization of Human Prostate Epithelial Cells
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Human prostate epithelial cells RWPE-1 was purchased from Procell (Wuhan, China) and cultured in K-SFM mediates with supplemented with 0.05 mg/mL BPE and 5 ng/mL EGF and 1% P/S (PB180120). Primary cultures of human prostate epithelial cells (HPEC) established from prostatectomy specimens represent prostate progenitor cells or transit ampli cation cells. In short, the tissue was minced and digested with collagenase overnight. Inoculate the digested tissue into a petri dish coated with type I collagen and containing PFMR-4A medium supplemented with 10 ng/ml cholera toxin, 10 ng/ml epidermal growth factor, 40 µg/ml bovine pituitary extract, 4 µg/ml insulin, 1 µg/ml hydrocortisone, 100 µg/ml gentamicin, 0.1 mm phosphoethanolamine, 3 nm selenious acid, 2.3 µ m α-tocopherol, and 0.03 nm total reaction Formula retinoic acid. The cells grown in the primary culture were aliquoted and stored frozen in liquid nitrogen. The epithelial properties of these cells were con rmed by immunocytochemical staining of cytokeratin 18 .
4
Cultivation of Prostate Cell Lines
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Human PCa cell line DU145 (Procell CL-0075) and normal prostatic epithelial cell line RWPE-1(Procell CL-0200) were kindly provided by Procell Life ScienceandTechnology Co., Ltd. The DU145 cell line was cultured in MEM medium (Biological Industries, Beit HaEmek, Israel), supplemented with 10% fetal bovine serum in a standard humidified incubator at 37°C in a 5% CO2 atmosphere. The RWPE-1 cell line was cultured in RPMI-1640 medium (Biological Industries, Beit HaEmek, Israel) supplemented with 10% fetal bovine serum at 37°C in a humidified atmosphere containing 5% CO2.
5
Prostate Cancer Cell Line Cultivation
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Prostate cancer cell lines DU145, PC-3, and LNCaP and normal adult human prostatic epithelial cells RWPE-1 all supplied by Procell (Wuhan, China). All four cell lines were grown in 1640 complete medium. All of the cell types grew in an incubator at 37 °C with 5% CO2. Elesclomol (MCE, United States) was diluted to a 100 nmol/L concentration in a cell culture medium. R1881 (RHAWN Chemical Technology Co., Ltd Shanghai China) was diluted to a 0.1 nmol/L concentration in a cell culture medium.
6
Isolation and Characterization of Human Prostate Epithelial Cells
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Human prostate epithelial cells RWPE-1 was purchased from Procell (Wuhan, China) and cultured in K-SFM mediates with supplemented with 0.05 mg/mL BPE and 5 ng/mL EGF and 1% P/S (PB180120). Primary cultures of human prostate epithelial cells (HPEC) established from prostatectomy specimens represent prostate progenitor cells or transit ampli cation cells. In short, the tissue was minced and digested with collagenase overnight. Inoculate the digested tissue into a petri dish coated with type I collagen and containing PFMR-4A medium supplemented with 10 ng/ml cholera toxin, 10 ng/ml epidermal growth factor, 40 µg/ml bovine pituitary extract, 4 µg/ml insulin, 1 µg/ml hydrocortisone, 100 µg/ml gentamicin, 0.1 mm phosphoethanolamine, 3 nm selenious acid, 2.3 µ m α-tocopherol, and 0.03 nm total reaction Formula retinoic acid. The cells grown in the primary culture were aliquoted and stored frozen in liquid nitrogen. The epithelial properties of these cells were con rmed by immunocytochemical staining of cytokeratin 18 .
7
Culturing Prostate Cancer Cell Lines
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Human prostate cancer cells PC3, DU145, and LNCAP and human normal prostate epithelium cell line RWPE-1 were purchased from Procell (Procell Life Science and Technology Co., Ltd.). Cells were cultured in RPMI-1640 medium (Invitrogen) mixed with 10% FBS. The incubator was set as a water-saturated atmosphere with 5% CO2 at 37°C.
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