Eg1150h machine

Manufactured by Leica camera

The EG1150H Machine is a product from Leica, a leading manufacturer of high-quality lab equipment. It serves as a core function of tissue processing, preparing samples for further analysis and examination.

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Lab products found in correlation

Autostainer xl, by Leica camera (1 mentions) Cv5030, by Leica camera (1 mentions) Nis elements d software, by Nikon (1 mentions) Fdx 35, by Nikon (1 mentions)

2 protocols using eg1150h machine

Adipose Tissue Histological Analysis

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Adipose samples were fixed in formalin and paraffin embedded. Sections were prepared (5 μM) using Leica EG1150H Machine. Haematoxylin and Eosin (H&E) staining was conducted using Leica Autostainer XL and Leica CV5030. Sections were mounted using DPX media (Fisher Scientific, Ireland) and analyzed using Nikon 80i transmission light microscope.

Lima E.A., Silveira L.S., Masi L.N., Crisma A.R., Davanso M.R., Souza G.I., Santamarina A.B., Moreira R.G., Roque Martins A., de Sousa L.G., Hirabara S.M, & Rosa Neto J.C. (2014). Macadamia Oil Supplementation Attenuates Inflammation and Adipocyte Hypertrophy in Obese Mice. Mediators of Inflammation, 2014, 870634.

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Placental Histology: H&E Staining

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Placental samples were immediately fixed in 10% neutral-buffered formalin and paraffin embedded. Sections were prepared (5 μm) using Leica EG1150H Machine. Sections were stained for Hematoxylin and Eosin (H&E) using standard procedures. Sections were analyzed under light microscope (Nikon 800, Tokyo, Japan) and images taken (Nikon FDX-35, Tokyo, Japan) and processed with NIS Elements-D software (Nikon). Placental zone analysis was performed using ImageJ software analysis at magnifications (×1.25) with four sections per placenta).

Reynolds C.M., Vickers M.H., Harrison C.J., Segovia S.A, & Gray C. (2015). Maternal high fat and/or salt consumption induces sex-specific inflammatory and nutrient transport in the rat placenta. Physiological Reports, 3(5), e12399.

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