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Antibody against erα

Manufactured by Santa Cruz Biotechnology
Sourced in United States

The Antibody against ERα is a research-grade antibody specifically designed to detect the presence of the Estrogen Receptor alpha (ERα) protein in biological samples. This antibody can be used in various immunodetection techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to study the expression and localization of ERα in cells and tissues.

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2 protocols using antibody against erα

1

Antibody and Inhibitor Procurement for Signaling Pathway Analysis

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Antibody against ERα was obtained from Santa Cruz Biotechnology. ERβ–MC-10 antibody was developed and characterized by Dr. Hawse’s laboratory (Wu et al., 2012 (link)). Fluorescent secondary antibodies were obtained from Li-Cor Biosciences (Lincoln, NE). Pharmacological inhibitors for the signaling pathways NFκB (SN50), P42/44 (PD98059), PI3K (Wortmannin) and P38 (SB203580) were obtained from EMD/Calbiochem (Culver City, CA), STAT6 (AS1517499) from Axon MedChem (Reston, VA), and AP1 (SR11302) from Tocris (Minneapolis, MN). TNFα and IL-13 were obtained from R&D systems (Minneapolis, MN). All other chemicals or antibodies were obtained from Sigma-Aldrich.
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2

Antibody-based Protein and miRNA Analysis

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Antibody against ERα was from Santa Cruz (Santa Cruz, CA, USA), antibody against α-tubulin from Sigma (St Louis, MO, USA). All other reagents were from Sigma. Anti-miR-26a and nonsense of anti-miR were from GenePharma (Shanghai, PR China). Cel-miR-39 mimics were from IBS (Shanghai, PR China).
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