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The AB2175 is a laboratory equipment product manufactured by Cell Signaling Technology. It is a specialized device designed for use in scientific research and experimentation. The core function of the AB2175 is to perform a specific task or procedure required in the study of cellular processes and biological systems. No further details about the intended use or capabilities of the product can be provided in an unbiased and factual manner.

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Lab products found in correlation

Ab214, by Merck Group (2 mentions) Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Ab79398, by Cell Signaling Technology (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Irdye 680cw donkey, by LI COR (1 mentions) Ripa buffer, by Merck Group (1 mentions) Phosphatase inhibitor cocktail 2, by Merck Group (1 mentions) Phosphatase inhibitor cocktail 3, by Merck Group (1 mentions) Mini protean tgxtm precast gel, by Bio-Rad (1 mentions) Sc 8408, by Cell Signaling Technology (1 mentions)

2 protocols using ab2175

1

Protein Extraction and Western Blot Analysis

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Cells were harvested and lysed in RIPA buffer (R0278, Sigma.UK) with the addition of protease cocktail inhibitor (P8348, Sigma, UK), phosphatase inhibitor cocktail 2 (P5726, Sigma, UK) and phosphatase inhibitor cocktail 3 (P0044, Sigma) and stored at −20 °C. Proteins were quantified using BCA Protein Assay kit (23225, Thermofisher, UK). Samples were run on SDS-bolt gel (4–12%) bis-tris. Membranes were incubated with primary antibodies as follows: Mre11 (1:500, ab214), ß-actin (1:1000, ab8226), YY1 (1:1000, ab109228), GADPH (1:1000, ab9485), XRCC1 (1:1000, ab1838), pChk1 (1:1000, ab58567), Rad50 (1:1000, ab89), NBS1 (1:1000, Sigma, APREST78218), XRCC1 (1:500, ab1838), FEN-1 (1:1000, NB100-150), PARP (1:1000, 46D11 Cell Signaling), LIG1 (1:1000, ab177946), LIG3 (1:1000, HPA006723), RPA1 (1:1000, ab79398), RPA2 (1:1000, AB2175) and RPA3 (1:1000, ab97436). Membranes then were washed and incubated with Infrared dye-labelled secondary antibodies (LiCor) [IRDye 800CW Donkey Anti-Rabbit IgG (926-32213) and IRDye 680CW Donkey Anti-Mouse IgG (926-68072)] at dilution of 1:10,000 for 1 h. Membranes were scanned with a LiCor Odyssey machine (700 and 800 nm) to determine protein levels. Blots showed derived from the same experiments.
Alblihy A., Ali R., Algethami M., Shoqafi A., Toss M.S., Brownlie J., Tatum N.J., Hickson I., Moran P.O., Grabowska A., Jeyapalan J.N., Mongan N.P., Rakha E.A, & Madhusudan S. (2022). Targeting Mre11 overcomes platinum resistance and induces synthetic lethality in XRCC1 deficient epithelial ovarian cancers. NPJ Precision Oncology, 6, 51.
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2

Comprehensive Protein Detection Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
SDS-PAGE and western blotting were performed using BioRad 4–15% Mini-PROTEAN TGXTM Precast Gels. Additional SDS-PAGE and western blotting was done using Bolt 4–12% Bis-Tris Plus gels from Thermo Fisher Scientific. The following antibodies were used at the indicated dilutions: BLM (goat, Abcam, ab5446, 1/500), GFP (mouse, Roche, 11814460001, 1/5000), HA (mouse, Santa Cruz Biotechnology, sc-57592, 1/1000), NBS1 (rabbit, Abcam, ab32074, 1/2000), NBS1 (mouse, GeneTex, GTX70224, 1/1000), TOPBP1 (A300-111A, Bethyl Laboratories, 1/5000), TOPBP1 (rabbit, Abcam, Ab2402, 1/1500), ATM pS1981 (rabbit, Epitomics, 2152-1, 1/5000), ATM (rabbit, Calbiochem, PC-116, 2/250), Treacle (rabbit, Sigma, HPA038237, 1/500), RAD9 (rabbit, Abcam, ab70810, 1/3000), RAD50 (mouse, GeneTex, 13B3, GTX70288, 1/1000), MRE11 (mouse, Abcam, 12D7, ab214, 1/500), Tubulin (mouse, Sigma, DM1A; T6199, 1/2000), RPA-pS4/S8 (rabbit, Bethyl laboratories, A700-009, 1/1000), RPA2 (mouse, Abcam, ab2175, 1/10000), CHK1 pS317 (rabbit, Cell Signaling, 2344, 1/500), CHK1 pS345 (rabbit, Cell Signaling, 2348, 1/500) CHK1 (mouse, Santa Cruz Biotechnology, sc-8408, 1/1000), CHK2 pT68 (rabbit, Cell Signaling, 2661, 1/1000), CHK2 (rabbit, Cell Signaling, 2662, 1/1000).
Mooser C., Symeonidou I.E., Leimbacher P.A., Ribeiro A., Shorrocks A.M., Jungmichel S., Larsen S.C., Knechtle K., Jasrotia A., Zurbriggen D., Jeanrenaud A., Leikauf C., Fink D., Nielsen M.L., Blackford A.N, & Stucki M. (2020). Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation. Nature Communications, 11, 123.
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