PEGylated cypate in methanol was dried under rotary evaporation, and the resulting thin film was hydrated with 1 ml of PBS (pH 7.4) and vortexed. After sonication for 10 min, large aggregates were removed by filtration through 0.2-μm polycarbonate membrane filters (Millipore Corp., Billerica, MA), and SP3NPs were obtained and stored at 4 oC until use. The size and morphology of SP3NPs were examined by TEM using a JEM1010 system (Jeol Ltd, Tokyo, Japan). The hydrodynamic diameters and zeta potentials of SP3NPs were measured using a Nanosizer ZS90 instrument (Malvern Instruments Ltd, Malvern, UK). The colloidal stability of SP3NPs in PBS and 50% FBS was tested by monitoring changes in size over 1 wk. The UV-Vis spectra of SP3NPs were recorded using a UV-Vis spectrophotometer (NEOSYS2000; Scinco, Twin Lakes, WI).
Jem 1010 system
Manufactured by JEOL
Sourced in Japan
The JEM-1010 system is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of various samples. It provides a stable and reliable platform for researchers and scientists to study the microstructure and composition of materials at the nanoscale level.
Automatically generated - may contain errors
6 protocols using jem 1010 system
1
Characterization of Stabilized Fluorescent Nanoparticles
2
Characterization of Nanomaterials via TEM, XRD, UV-Vis, and VSM
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Transmission electron microscopy (TEM) was performed on a JEM-1010 system (JEOL, Tokyo, Japan). Powder X-ray diffraction (XRD) analyses were carried out using a Bruker D8 Advance diffractometer with Cu Kα radiation and the scanning angle ranged from 10° to 80° of 2θ. UV absorbance data were acquired from a U-3010 UV spectrophotometer equipped a 1 cm cuvette (Kyoto, Japan). The magnetic properties were measured with a vibrating sample magnetometer (VSM) (LDJ 9600-1, USA). The magnetic properties were measured with a vibrating sample magnetometer (VSM) (LDJ 9600-1, USA).
3
Electron Microscopy Analysis of Materials
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The morphology and dimensions of the materials were also analyzed in a JEOL JEM-1010 system operating at 120 kV.
4
Characterization of Coated Lipid Nanoparticles
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C-LPNs were characterized with respect to morphology, elemental mapping, size, zeta potential, lipid coating, and antibody conjugation efficiency. The morphology of C-LPNs was visualized by transmission electron microscopy (TEM) using a JEM1010 system (JEOL, Tokyo, Japan). Prior to visualization, C-LPNs were briefly stained with a 1% uranyl acetate solution. Elemental mapping of carbon, oxygen, nitrogen and phosphorus present in C-LPNs was performed using energy dispersive X-ray spectroscopy-scanning transmission electron microscopy (EDS-STEM) using a JEM-2100F system (JEOL). Hydrodynamic size, size distribution, and zeta potentials were measured using dynamic light scattering and laser Doppler microelectrophoresis at an angle of 22° using an ELS8000 instrument (Photal, Osaka, Japan). The lipid content of nanoparticles was quantified by measuring phosphorus content using a phosphate assay18 (link). The content of immobilized antibody on nanoparticles was measured using a Cedex Bio Analyzer (Roche). The photothermal ability of LPNs was investigated by irradiating with a 808 nm laser using a diode laser beam (BWT Beijing Ltd., Beijing, China) at an output power of 1.5 W. Real-time temperatures were measured using an infrared camera (FLIR E60; FLIR Systems Inc., Danderyd, Sweden).
5
Particle Morphology and Dimensions
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The morphology and dimensions of the materials were also analyzed in a JEOL JEM-1010 system operating at 120 kV. Particles were measured using ImageJ software and the resulting histograms were constructed by the OriginPro 8.5 (OriginLab, Northampton, MA, USA) software package.
6
Transmission Electron Microscopy and UV Spectroscopy
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Transmission electron microscopy (TEM) was carried out on a JEM-1010 system (JEOL, Japan). UV absorbance data were measured on a U-3010 UV spectrophotometer (Kyoto, Japan).
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