Ultrasensitive mouse elisa kits

Blood samples were collected through cardiac puncture immediately following overdose with isoflurane, placed into an EDTA microvette tube, and spun at 1500 G for 10 min at 4°C. The separated plasma was stored in a – 80°C freezer until further use. Blood chemistry analysis of circulating insulin and adiponectin concentration was carried out using Ultra-Sensitive Mouse ELISA kits (Crystal Chem, IL; Fujifilm, Japan) per the manufacturer’s instructions. The Human/Rat β Amyloid (42) ELISA Kit (Fujifilm, Wako) was utilized to determine hippocampal soluble Aβ₄₂ concentrations (experimental samples diluted 1:50). Hippocampal tissue was homogenized in 50 mM Tris Buffer (pH = 7.6) with protease inhibitor and centrifuged for 20 min at 4°C. Homogenates were aliquoted and stored at – 80°C until quantification.