Celltiter glo 96 aqueous one solution cell proliferation assay

To determine ATP effects of etomoxir treatment, tumor cells were seeded in 96-well plates at 5,000–7,000 cells per well and cultured in the presence of 0 or 200 µM etomoxir (Sigma-Aldrich) for 48 h, with triplicate samples for each condition. Relative ATP concentration was determined using the CellTiter-Glo Luminescent Cell Viability Assay (Promega). To determine NAD(P)H effects of etomoxir treatment, HMEC^MYC-ER cells were seeded in 96-well plates at 2,000 cells per well and cultured in the presence of 0 or 500 nM TAM for 48 h, then 0 or 200 µM etomoxir for 24 h, with six samples for each condition. Relative NAD(P)H concentration was determined using CellTiter-Glo 96 AQueous One Solution Cell Proliferation Assay (Promega).