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Cd45 clone j33

Manufactured by Beckman Coulter
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CD45 (clone J33) is a monoclonal antibody used for the identification and enumeration of leukocytes. It is designed for use with Beckman Coulter flow cytometry instruments and software.

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Lab products found in correlation

Cd4 clone okt4, by Thermo Fisher Scientific (1 mentions) Granzyme b clone gb11, by Thermo Fisher Scientific (1 mentions) Hla dr clone ln3, by Thermo Fisher Scientific (1 mentions) Navios, by Beckman Coulter (1 mentions) Cd38 clone ls198.4.3, by Beckman Coulter (1 mentions) Facscanto, by BD (1 mentions) Hla dr clone immu 357, by Beckman Coulter (1 mentions) Cd33 clone p67.6, by BD (1 mentions) Cd45ra clone hi100, by BD (1 mentions) Cd45ra 2h4, by Beckman Coulter (1 mentions) Cd4 13b8.2, by Beckman Coulter (1 mentions) Cd3 ucht 1, by Beckman Coulter (1 mentions) Cd7 8h8.1, by Beckman Coulter (1 mentions) Cd25 b1, by Beckman Coulter (1 mentions) Kaluza analysis software, by Beckman Coulter (1 mentions) Immu 357, by Beckman Coulter (1 mentions)

Close spelling variants of this product

CD45 (J33, (5 citations) CD45 KromeOrange-conjugated (clone J33; (2 citations)

4 protocols using cd45 clone j33

1

Multiparameter Flow Cytometry Immunophenotyping

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The following fluorochrome-labeled mouse monoclonal antibodies were from Becton Dickinson (BD Biosciences, San Jose, CA, USA): anti-CD8 (clone RPA-T8), IFN-γ (clone: 4S.B3), IL-2 (clone MQ1-17H12), MIP-1β (clone D21-1351) and granzyme B (clone GB11); and from eBioscience (San Diego, CA): anti- CD3 (clone UCHT1), CD16 (clone CB16), CD56 (clone CMSSB), HLA-DR (clone LN3), CD38 (clone HIT2), CD69 (clone FN50), TNF-α (clone MAb11) and CD4 (clone OKT-4). In addition, we used CD45 (clone J.33) from Beckman Coulter (Fullerton, CA, USA) and perforin (clone B-D48) from BioProducts (Middletown, MD, USA).
Taborda N.A., González S.M., Alvarez C.M., Correa L.A., Montoya C.J, & Rugeles M.T. (2015). Higher Frequency of NK and CD4+ T-Cells in Mucosa and Potent Cytotoxic Response in HIV Controllers. PLoS ONE, 10(8), e0136292.
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2

CD47 Expression Analysis in AML

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CD47 expression levels on primary AML samples were analyzed by flow cytometry (Navios, Beckman Coulter, Krefeld, Germany) using the mAbs CD47 (clone 472603, R&D), CD45 (clone J33, Beckman Coulter), CD34 (clone 581, Beckman Coulter) and CD38 (clone LS198-4-3, Beckman Coulter) and the corresponding isotype controls. Bulk AML cells were identified by limiting the analysis of primary AML sample material to SSClow/CD45dim cells. LSCs were defined as a CD34+/CD38− subgroup of SSClow/CD45dim cells. As a measure for CD47 intensity, CD47 MFI ratio was calculated using FlowJo software (Version 9.6, Tree Star Inc., Ashland, Oregon).
Ponce L.P., Fenn N.C., Moritz N., Krupka C., Kozik J.H., Lauber K., Subklewe M, & Hopfner K.P. (2017). SIRPα-antibody fusion proteins stimulate phagocytosis and promote elimination of acute myeloid leukemia cells. Oncotarget, 8(7), 11284-11301.
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3

Phenotypic Characterization of PBMCs and BMMCs

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Single-cell suspension of PBMC and BMMC cells were stained with monoclonal antibodies purchased from Miltenyi Biotec (Germany) including CD123 (clone AC145), CD303 (BDCA-2, clone AC144), CD304 (Neurophilin-1/BDCA-4, clone AD5-17F6), HLA-DR (clone Immu-357, Beckman Coulter), CD45 (clone J33, Beckman Coulter), CD45RA (clone HI100, BD PharmingenTM), CD33 (clone P67.6, BD Biosciences) and CD38 (clone HIT2, Exbio, Prague, Czech Republic). Samples were acquired using FACS Canto® (BD Biosciences) and data were analyzed using FlowJo® software (FlowJo, Ashland, OR, USA). Forward and side scatter gating were used to discriminate live cells from dead cells and were derived from SSC vs. FSC gated bulk PBMCs or BMMCs with doublet exclusion (FSC-A vs. FCS-H). To determine the placement of the gates, appropriate fluorescence minus one (FMO) and unstained controls were used.
Knight A., Rihova L., Kralova R., Penka M., Adam Z., Pour L., Piskacek M, & Hajek R. (2021). Plasmacytoid Dendritic Cells in Patients with MGUS and Multiple Myeloma. Journal of Clinical Medicine, 10(16), 3717.
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4

Multiparametric Flow Cytometry of Mucosal Lymphocytes

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PBMCs or lymphocytes isolated from the colon or rectal mucosa were utilized for flow cytometry. Monoclonal antibodies against CD45 (clone J33; Beckman Coulter, Pasadena, CA, USA), CD3 (UCHT1, Beckman Coulter), CD4 (13B8.2; Beckman Coulter), CD8 (B9.11; Beckman Coulter), CD7 (8H8.1; Beckman Coulter), CD25 (B1.49.9; Beckman Coulter), CD30 (HRS4; Beckman Coulter), CD45RA (2H4; Beckman Coulter), CD56 (N901; Beckman Coulter), CD62L (DREG56; Beckman Coulter), CD127 (R34.34; Beckman Coulter), CCR4 (i.e., CD194; L291H4; BioLegend), HLADR (Immu-357; Beckman Coulter), and PD1 (CD279; PD1.3; Beckman Coulter) were employed. The immunostained cells were analyzed using FACScan (Navios flow cytometer, Beckman Coulter) and Kaluza analysis software (version 1.3; Beckman Coulter). Lymphocytes were separated by flow cytometry based on high CD45 antigen expression and forward and side scatter properties. Subsequently, the flow cytometry data were analyzed according to the percentage of cell populations detected in each quadrant on two-dimensional scatterplots. We calculated the percentages of CD4+, CD8+, CD56+, CD7+, PD1+, CCR4+, CD30+, and HLADR+ cells among CD3+ cells. We also assessed the percentages of Treg, CD45RA+, and CD62L+ cells among CD3+CD4+ cells and percentages of CD45RA+ and CD62L+ cells among CD3+CD4− cells. In this study, we defined CD3+CD4+CD25+CD127low/- cells as Treg cells.
Iwamuro M., Takahashi T., Watanabe N., Tanaka T., Inokuchi T., Hiraoka S., Otsuka F, & Okada H. (2022). Enriched CD45RA−CD62L+ central memory T and decreased CD3+CD56+ natural killer T lymphocyte subsets in the rectum of ulcerative colitis patients. International Journal of Immunopathology and Pharmacology, 36, 20587384211051982.
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