Eravacycline

Manufactured by MedChemExpress

Sourced in China

Eravacycline is a tetracycline-class antibacterial agent. It is a synthetic fluorocycline that exhibits broad-spectrum activity against a variety of Gram-positive and Gram-negative bacteria, including multidrug-resistant pathogens.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using eravacycline

Compound Sourcing and Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

All compounds were obtained from the Cayman Chemical Company with some exceptions. Eravacycline was obtained from MedChemExpress. Flufenoxuron, kanamycin, tetracycline, ampicillin, haloperidol, and risperidone were purchased from the Sigma-Aldrich.
All compounds were dissolved in the recommended solvent (DMSO, DMF, alcohol, or water). Compounds were diluted in serum-free DMEM-N2 to the desired concentrations before addition to media.

Kao H.T., Orry A., Palfreyman M.G, & Porton B. (2022). Synergistic interactions of repurposed drugs that inhibit Nsp1, a major virulence factor for COVID-19. Scientific Reports, 12, 10174.

+ Open protocol

+ Expand

Profiling Carbapenem-resistant A. baumannii in China

Check if the same lab product or an alternative is used in the 5 most similar protocols

From January 2020 to December 2022, 287 non-duplicate A. baumannii isolates, including 147 CRAB and 140 carbapenem-susceptible A. baumannii (CSAB) isolates, were collected from various clinically sampled infections of inpatients at Yangjiang People’s Hospital, a tertiary hospital with 2000 beds in Guangdong Province of China. Bacterial species were identified using a VITEK 2 compact system (BioMérieux, Marcy l’Etoile, France). All strains in this study were cultured on Mueller-Hinton (MH) broth (Oxoid, Basingstoke, UK), or in MH agar plates and Luria-Bertani (LB) broth (Oxoid, Basingstoke, UK) at 37°C. All procedures performed were approved by the Ethical Committee of Yangjiang People’s Hospital and were in accordance with the 1964 Helsinki Declaration and its later amendments. The novel fluorocycline antibiotic, eravacycline (catalog no. HY-16980A), was purchased from MedChem Express (MCE, Shanghai, China). The other antimicrobials were purchased from Meilunbio (Dalian, China). Escherichia coli ATCC 25922 was used for quality control.

Li Y.T., Chen X.D., Guo Y.Y., Lin S.W., Wang M.Z., Xu J.B., Wang X.H., He G.H., Tan X.X., Zhuo C, & Lin Z.W. (2024). Emergence of eravacycline heteroresistance in carbapenem-resistant Acinetobacter baumannii isolates in China. Frontiers in Cellular and Infection Microbiology, 14, 1356353.

+ Open protocol

+ Expand

Antimicrobial Susceptibility Testing of Eravacycline and Tigecycline

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antimicrobial susceptibility testing was performed by the broth microdilution method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines.19 All isolates tested were stored in a −80 °C refrigerator. Eravacycline and tigecycline (MedChemExpress, Shanghai, China) were freshly prepared in sterile water on the day of use. The final inoculum of 1.5×10⁸ CFU/mL was acquired by adjusting the turbidity of the incubated mixture to 0.5 McFarland standards using normal saline.20 (link) Aliquots (100 μL) of the colony suspension were inoculated into 96-well broth microdilution panels (Corning, NY, USA) containing 200 μL aliquots of Eravacycline and tigecycline (<0.0625, 0.0625, 0.125, 0.25, 0.5, 1, 2, 4, 8, and 16 mg/L). And 96-well broth microdilution panels were read visually after incubation for 16–20 hours at 37 °C. The results of this study were interpreted according to the criteria of the European Committee on Antimicrobial Susceptibility (EUCAST) (

http://www.eucast.org

) and the Food and Drug Administration (FDA) (

https://www.fda.gov/

).21 ,22
E. coli ATCC 25922 was used as a quality control strain.

Zou X., Jin S., Chen L., Li J., Zhang X., Zhou H., Li X, & Huang H. (2023). Antibacterial Activity of Eravacycline Against Carbapenem-Resistant Gram-Negative Isolates in China: An in vitro Study. Infection and Drug Resistance, 16, 2271-2279.

+ Open protocol

+ Expand

Reconstitution of E. coli 70S Ribosomes

Check if the same lab product or an alternative is used in the 5 most similar protocols

In vitro reconstituted E. coli 70S ribosomes were generated from the E. coli K12 strain BW25113, as described previously^{56 (link)}. Antibiotic–ribosome samples were generated by incubating antibiotic cocktails 1–5 with E. coli 70S ribosomes in buffer A (50 mM HEPES-KOH, pH 7.5, 25 mM Mg(OAc)₂, 80 mM NH₄Cl, 100 mM KOAc, 1 mM DTT, 0.05% DDM) at 37 °C for 15 min, before being frozen at −80 °C until use. Final antibiotic concentrations for complexes formed with each cocktail was: cocktail 1 contained 200 μM omadacycline (MedChemExpress), 200 μM spectinomycin (Santa Cruz Biotechnology), 200 μM streptomycin (Santa Cruz Biotechnology), 200 μM evernimicin, 200 μM hygromycin B (Cayman Chemical); cocktail 2 contained 100 μM capreomycin (Sigma Aldrich), 100 μM kasugamycin (Sigma Aldrich) and 100 μM retapamulin (Sigma Aldrich); cocktail 3 contained 100 μM tetracycline (Sigma Aldrich), 100 μM viomycin (Sigma Aldrich), 100 μM streptomycin (Santa Cruz Biotechnology), 100 μM lincomyin (Sigma Aldrich) and 100 μM avilamycin (Cayman Chemical); cocktail 4 contained 10 μM apramycin (Sigma Aldrich), 10 μM eravacycline (MedChemExpress) and 100 μM clindamycin (Santa Cruz Biotechnology); cocktail 5 contained 100 μM pentacycline (Tetraphase), 10 μM gentamicin (Carl Roth) and 100 μM tiamulin (Sigma Aldrich).

Paternoga H., Crowe-McAuliffe C., Bock L.V., Koller T.O., Morici M., Beckert B., Myasnikov A.G., Grubmüller H., Nováček J, & Wilson D.N. (2023). Structural conservation of antibiotic interaction with ribosomes. Nature Structural & Molecular Biology, 30(9), 1380-1392.

+ Open protocol

+ Expand

Rifamycin Compound Library Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The 10 compounds in rifamycin
class used in this study were kindly provided by the Korea Chemical
Bank (www.chembank.org)
of the Korea Research Institute of Chemical Technology (Table S27). Rifamycin S (S4425), dirithromycin
(S4213), gamithromycin (S5328), spiramycin (S5744), kitasamycin (S3645),
acetylspiramycin (S3618), and josamycin (S4421) were purchased from
Selleckchem. Telithromycin (HYA0062), erythromycin ethylsuccinate
(HYB0957), tylosin (HYB0519A), midecamycin (HYB1908), tilmicosin (HYB0905),
tulathromycin A (HY15662), cadazolid (HY100436), radezolid (HY14800),
delplazolid (HY100180), lefamulin (HY16908A), pleuromutilin (HYN2301),
azamulin (HYW019847), valnemulin (HYB0027), chlortetracycline (HYB1327A),
demeclocycline (HY121268), eravacycline (HY16980), rolitetracycline
(HY18257), minocycline (HY17412A), meclocycline (HYB1366), omadacycline
(HY14865), and sarecycline (HY13858A) were purchased from MedChemExpress.
Troleandomycin (ab141568) was purchased from Abchem. Eperezolid (B3390)
was purchased from APExBIO. BODIPYTR Cadeverine (D6251) was purchased
from Invitrogen. Other compounds and E. coli O128:B12
LPS were purchased from Sigma-Aldrich.

Choi Y., Choe H.W., Kook M., Choo S., Park T.W., Bae S., Kim H., Yang J., Jeong W.S., Yu J., Lee K.R., Kim Y.S, & Yu J. (2023). Proline-Hinged α-Helical Peptides Sensitize Gram-Positive Antibiotics, Expanding Their Physicochemical Properties to Be Used as Gram-Negative Antibiotics. Journal of Medicinal Chemistry, 67(3), 1825-1842.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!