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Anti mouse igg isotype control

Manufactured by Santa Cruz Biotechnology
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The Anti-mouse IgG isotype control is a laboratory reagent used to establish baseline signal levels in immunoassays involving mouse antibodies. It serves as a negative control to differentiate specific antibody binding from non-specific background signals.

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Lab products found in correlation

Lysis buffer, by Cell Signaling Technology (2 mentions) Anti mouse tlr7, by Thermo Fisher Scientific (2 mentions) Anti mouse myd88, by Santa Cruz Biotechnology (2 mentions)

3 protocols using anti mouse igg isotype control

1

TLR7-MyD88 Co-immunoprecipitation in BMDCs

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Co-immunoprecipitation experiments were performed using BMDCs seeded in 6-well plates at a final concentration of 1×106 cells/mL in RPMI-complete media. Cells were incubated with 10 μg/mL Gardiquimod for 15 minutes at 37C with 5% CO2. Cells were harvested and lysed in 1× lysis buffer (Cell Signaling). Lysates were incubated with anti-mouse TLR7 (Fisher Scientific) or anti-mouse IgG isotype control (Santa Cruz Biotech) for 24 h at 4C. Supernatants were incubated with Protein G agarose beads overnight at 4C. Precipitated proteins are eluted, separated by SDS-PAGE and immunoblotted with anti-mouse MyD88 (Santa Cruz Biotech).
Ramirez-Ortiz Z.G., Prasad A., Griffith J.W., Pendergraft WF I.I.I., Cowley G.S., Root D.E., Tai M., Luster A.D., Khoury J.E., Hacohen N, & Means T.K. (2015). TREML4 amplifies TLR7-mediated signaling during antiviral responses and autoimmunity. Nature immunology, 16(5), 495-504.
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2

Satellite Cell Intracellular Signaling Analysis

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Satellite cells were isolated as described above. FITC anti‐Sca1 instead of PE/Cy7 anti‐Sca1 and APC‐Cy7 streptavidin instead of PE streptavidin were used for the staining. Then immunofluorescence staining of intracellular phosphor‐Akt (Ser473; CST) and pS6 (CST) for flow cytometric analysis were performed according to the manufacture's protocol. Immunofluorescence staining for PIP3, cells stained with above antibodies were fixed in 4% formaldehyde for 20 min at RT, washed three times with PBS, and then permeabilized with 0.2% Triton X‐100 in PBS for 10 min at RT. Samples were blocked with 3% IgG‐free BSA and 0.1% Triton X‐100 in PBS for 1 h at RT, followed by incubation with antibody against PIP3 (Echelon Biosciences) or anti mouse IgG isotype control (Santa Cruz) at RT for 90 min. After washing with PBS, the samples were incubated with secondary antibodies (Key Resources Table) for 30 min at RT followed by FACS analysis.
Liu J., Zuo H., Wang Z., Wang W., Qian X., Xie Y., Peng D., Xie Y., Hong L., You W., Lou H., Luo G., Ren J., Shen B., Zheng J., Wang H, & Ju Z. (2023). The m6A reader YTHDC1 regulates muscle stem cell proliferation via PI4K–Akt–mTOR signalling. Cell Proliferation, 56(8), e13410.
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3

TLR7-MyD88 Co-immunoprecipitation in BMDCs

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Co-immunoprecipitation experiments were performed using BMDCs seeded in 6-well plates at a final concentration of 1×106 cells/mL in RPMI-complete media. Cells were incubated with 10 μg/mL Gardiquimod for 15 minutes at 37C with 5% CO2. Cells were harvested and lysed in 1× lysis buffer (Cell Signaling). Lysates were incubated with anti-mouse TLR7 (Fisher Scientific) or anti-mouse IgG isotype control (Santa Cruz Biotech) for 24 h at 4C. Supernatants were incubated with Protein G agarose beads overnight at 4C. Precipitated proteins are eluted, separated by SDS-PAGE and immunoblotted with anti-mouse MyD88 (Santa Cruz Biotech).
Ramirez-Ortiz Z.G., Prasad A., Griffith J.W., Pendergraft WF I.I.I., Cowley G.S., Root D.E., Tai M., Luster A.D., Khoury J.E., Hacohen N, & Means T.K. (2015). TREML4 amplifies TLR7-mediated signaling during antiviral responses and autoimmunity. Nature immunology, 16(5), 495-504.
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