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Human rcela2a

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Human rCELA2A is a recombinant protein representing the catalytic domain of the human CELA2A (chymotrypsin-like elastase family member 2A) enzyme. CELA2A is a serine protease that plays a role in the digestion of dietary proteins. The recombinant protein is produced in an E. coli expression system.

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Lab products found in correlation

Actin, by Cell Signaling Technology (2 mentions) P s6kt389, by Cell Signaling Technology (2 mentions) Total s6k, by Cell Signaling Technology (2 mentions) Cd41 fitc, by BioLegend (2 mentions) Annexin 5 fitc, by BioLegend (2 mentions) Rat insulin elisa kit, by ALPCO (2 mentions) Pakt t308, by Cell Signaling Technology (2 mentions) Cela2a elisa kit, by MyBioSource (2 mentions) Cela2a antibody, by Merck Group (2 mentions) N succinyl ala ala pro leu p nitroanilide substrate, by Merck Group (2 mentions) Fitc conjugated pac1 antibody, by BD (2 mentions) Tetramethylrhodamine ethyl ester tmre reagent, by Thermo Fisher Scientific (2 mentions) Total irs, by Cell Signaling Technology (2 mentions) Pirs y608, by Cell Signaling Technology (2 mentions) Pirs s636 s639, by Cell Signaling Technology (2 mentions) A1at antibody ab9400, by Abcam (2 mentions) Itga2b antibodies, by Abcam (2 mentions) Human c peptide elisa kit, by ALPCO (2 mentions) Human glucagon elisa kit, by Mercodia (2 mentions) Rat c peptide elisa, by ALPCO (2 mentions)

2 protocols using human rcela2a

1

Quantitative Platelet and Pancreatic Assays

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Purified GP2B3A complex (MBS135714), mouse rCela2a protein (MBS1246487), human rCELA2A (MBS1090462) and the CELA2A ELISA kit (MBS932150) were purchased from MyBiosource. CELA2A antibody (SAB1104798) and the elastase substrate N-Succinyl-Ala-Ala-Pro-Leu p-nitroanilide substrate (S8511) were purchased from Sigma-Aldrich. The FITC-conjugated PAC1 antibody (340507, BD Biosciences) and tetramethylrhodamine ethyl ester (TMRE) reagent (T669, Thermo Fisher Scientific), Annexin V-FITC (640905, Biolegend), CD41-FITC (303703, Biolegend) were used for platelet aggregation analysis. Total AKT (CST, 9272), pAKT (S473) (CST, 9271s), pAKT (T308) (CST, 9275s), total S6k (CST, 9202), pS6k (T389) (CST, 9206), total IRS (CST, 2382), pIRS (Y608), (CST, 2385S), pIRS (S636/S639) (CST, 2388s), Gapdh (CST, 3683s), and Actin (CST, 4970) antibodies were purchased from Cell Signaling Technologies. Recombinant A1AT (alpha1 antitrypsin, ab91136), A1AT antibody (ab9400) and ITGA2B antibodies (ab63983) were purchased from Abcam. Human c-peptide ELISA kit (80-CPTHU-E01.1, ALPCO), Human Glucagon ELISA kit (10-1271-01, Mercodia), Rat c-peptide ELISA (80-CPTRT-E01, ALPCO), Mouse Ultrasensitive Insulin ELISA (80-INSMSU-E01, ALPCO), Human Insulin ELISA kit (21-IAAHU-E01, ALPCO), Rat Insulin ELISA kit (80-INSRT-E01, ALPCO) was applied for C-peptide, glucagon, and insulin measurements.
Esteghamat F., Broughton J.S., Smith E., Cardone R., Tyagi T., Guerra M., Szabó A., Ugwu N., Mani M.V., Azari B., Kayingo G., Chung S., Fathzadeh M., Weiss E., Bender J., Mane S., Lifton R.P., Adeniran A., Nathanson M.H., Gorelick F.S., Hwa J., Sahin-Tóth M., Belfort-DeAguiar R., Kibbey R.G, & Mani A. (2019). CELA2A mutations predispose to early-onset atherosclerosis and metabolic syndrome and affect plasma insulin and platelet activation. Nature genetics, 51(8), 1233-1243.
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2

Quantitative Platelet and Pancreatic Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purified GP2B3A complex (MBS135714), mouse rCela2a protein (MBS1246487), human rCELA2A (MBS1090462) and the CELA2A ELISA kit (MBS932150) were purchased from MyBiosource. CELA2A antibody (SAB1104798) and the elastase substrate N-Succinyl-Ala-Ala-Pro-Leu p-nitroanilide substrate (S8511) were purchased from Sigma-Aldrich. The FITC-conjugated PAC1 antibody (340507, BD Biosciences) and tetramethylrhodamine ethyl ester (TMRE) reagent (T669, Thermo Fisher Scientific), Annexin V-FITC (640905, Biolegend), CD41-FITC (303703, Biolegend) were used for platelet aggregation analysis. Total AKT (CST, 9272), pAKT (S473) (CST, 9271s), pAKT (T308) (CST, 9275s), total S6k (CST, 9202), pS6k (T389) (CST, 9206), total IRS (CST, 2382), pIRS (Y608), (CST, 2385S), pIRS (S636/S639) (CST, 2388s), Gapdh (CST, 3683s), and Actin (CST, 4970) antibodies were purchased from Cell Signaling Technologies. Recombinant A1AT (alpha1 antitrypsin, ab91136), A1AT antibody (ab9400) and ITGA2B antibodies (ab63983) were purchased from Abcam. Human c-peptide ELISA kit (80-CPTHU-E01.1, ALPCO), Human Glucagon ELISA kit (10-1271-01, Mercodia), Rat c-peptide ELISA (80-CPTRT-E01, ALPCO), Mouse Ultrasensitive Insulin ELISA (80-INSMSU-E01, ALPCO), Human Insulin ELISA kit (21-IAAHU-E01, ALPCO), Rat Insulin ELISA kit (80-INSRT-E01, ALPCO) was applied for C-peptide, glucagon, and insulin measurements.
Esteghamat F., Broughton J.S., Smith E., Cardone R., Tyagi T., Guerra M., Szabó A., Ugwu N., Mani M.V., Azari B., Kayingo G., Chung S., Fathzadeh M., Weiss E., Bender J., Mane S., Lifton R.P., Adeniran A., Nathanson M.H., Gorelick F.S., Hwa J., Sahin-Tóth M., Belfort-DeAguiar R., Kibbey R.G, & Mani A. (2019). CELA2A mutations predispose to early-onset atherosclerosis and metabolic syndrome and affect plasma insulin and platelet activation. Nature genetics, 51(8), 1233-1243.
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