Venous blood was collected from the patients after an overnight fasting. Whole blood was collected and placed in tubes containing ethylenediaminetetraacetic acid (EDTA) as anticoagulant. The blood was immediately placed on ice or in a refrigerator, and next the samples were centrifuged at 3500 rpm for 10 min at 4°C within max 2 h of collection. Plasma was stored at −80°C. Standard blood biochemical analyses were performed in the University Hospital Laboratory. 5(S), 6(R)-lipoxin A4, 5(S), 6(R), 15(R)-lipoxin A4, 5(S)-HETE, 5(S)-oxoETE, 12(S)-HETE, 15(S)-HETE, 16(R)/16(S)-HETE, 9(S)-HODE, and 13(S)-HODE were extracted from 0.5 ml of plasma using a solid-phase extraction RP-18 SPE columns (Agilent Technologies, Cheadle, UK) [21 (link)]. Recovery and sensitivity of method were described previously [22 (link)]. The total recovery for all sample extraction and processing steps (mean ± SD) was 46 ± 8%.
Rp 18 spe columns
Manufactured by Agilent Technologies
Sourced in United Kingdom
The RP-18 SPE columns from Agilent Technologies are solid-phase extraction (SPE) columns designed for the purification and separation of a wide range of analytes. These columns utilize a C18 stationary phase for reversed-phase chromatography. The RP-18 SPE columns are suitable for various applications, including sample preparation, analyte concentration, and matrix removal.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using rp 18 spe columns
1
Plasma Lipid Mediator Extraction
2
Quantification of Lipid Mediators in Plasma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
5(S),6(R)-Lipoxin A4, 5(S),6(R), 15(R)- Lipoxin A4, 5(S)-HETE, 5(S)-oxoETE, 12(S)-HETE, 15(S)-HETE, 16(R)/16(S)-HETE, 9(S)-HODE and 13(S)-HODE were extracted from the 0.5 ml of plasma by using a solid-phase extraction RP-18 SPE columns (Agilent Technologies, UK).
The HPLC separations were performed using Agilent Technologies 1260 liquid chromatography. Agilent ChemStation software (Agilent Technologies, Cheadle, UK) was employed for instrument control, data acquisition and analysis. The separation was completed on a Thermo Scientific Hypersil BDS C18 column 100x4.6mm 3μm (cat no. 28103-104630). The temperature of the column oven was set at 25°C. A gradient method was used where the mobile phase was composed of a mixture of solvent A (methanol/water/acetic acid, 50/50/0.1, v/v/v) and B (methanol/water/acetic acid, 100/0/0.1, v/v/v). The content of buffer B in the mobile phase was 30% at 0.0 min of separation, then it increased linearly to 80% at 20 min, was 98% between 20.1 and 23.9 min, and 30% between 24 and 28 min. The flow rate was 1.0 mL/min. The sample injection volume was 60 uL. The absorbance spectra of peaks were analysed to confirm the identification of analytes. The quantification was based on peak areas with internal standard calibration.
The HPLC separations were performed using Agilent Technologies 1260 liquid chromatography. Agilent ChemStation software (Agilent Technologies, Cheadle, UK) was employed for instrument control, data acquisition and analysis. The separation was completed on a Thermo Scientific Hypersil BDS C18 column 100x4.6mm 3μm (cat no. 28103-104630). The temperature of the column oven was set at 25°C. A gradient method was used where the mobile phase was composed of a mixture of solvent A (methanol/water/acetic acid, 50/50/0.1, v/v/v) and B (methanol/water/acetic acid, 100/0/0.1, v/v/v). The content of buffer B in the mobile phase was 30% at 0.0 min of separation, then it increased linearly to 80% at 20 min, was 98% between 20.1 and 23.9 min, and 30% between 24 and 28 min. The flow rate was 1.0 mL/min. The sample injection volume was 60 uL. The absorbance spectra of peaks were analysed to confirm the identification of analytes. The quantification was based on peak areas with internal standard calibration.
3
Plasma Lipid Mediator Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following inflammatory mediators were analyzed: resolvin D1, maresine1, prostaglandin B2, 5(S),6(R), 15(R)-lipoxin A4, 5(S),6(R)-lipoxin A4, leucotriene B4, 16(RS)HEPE, 5(S)-HETE, 12(S)-HETE, 15(S)-HETE, 5(S)-oxoETE, 10(S)17(R)DiDHA, 16(R)/16(S)-HETE, 9(S)-HODE, 13(S)-HODE, 17(RS)HDHA. All derivatives were extracted from the 0.5ml of plasma by using a solid-phase extraction RP-18 SPE columns (Agilent Technologies, Santa Clara, CA, USA).
4
Bioactive Lipid Extraction from Plasma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lipoxin A4 (LX A4), 5-hydroxyeicosatetraenoic acid (5-HETE), 12-hydroxyeicosatetraenoic acid (12-HETE), 15-hydroxyeicosatetraenoic acid (15-HETE), 16-hydroxyeicosatetraenoic acid (16-HETE), 9-hydroxyloctadecadienoic acid (9-HODE), 13-hydroxyloctadecadienoic acid (13-HODE), 16-hydroxyeicosapentaenoic acid (16-HEPE), 17-hydroxydocosahexaenoic acid (17-HDHA), Protectin DX, Maresine1, Leucotriene B4, Prostaglandin B2, and resolvin D1 were extracted from the 0.5 mL of plasma by using a solid-phase extraction RP-18 SPE columns (Agilent Technologies, UK).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!